Comparative analysis of the diagnostic performance of crude sheep hydatid cyst fluid, purified antigen B and its subunit (12 Kda), assessed by ELISA, in the diagnosis of human cystic echinococcosis

2010 ◽  
Vol 108 (2) ◽  
pp. 371-376 ◽  
Author(s):  
Gihan M. Tawfeek ◽  
Hala S. Elwakil ◽  
Laila El-hoseiny ◽  
Hala S. Thabet ◽  
Rania M. Sarhan ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Hydatid Cyst ◽  
Cystic Echinococcosis ◽  
Diagnostic Performance ◽  
Cyst Fluid ◽  
Antigen B ◽  
Purified Antigen ◽  
Human Cystic Echinococcosis ◽  
Hydatid Cyst Fluid

Diagnostic value of semi-purified antigens of hydatid cyst fluid in human cystic echinococcosis

2016 ◽  
Vol 61 (1) ◽  
Author(s):  
Wissal Iraqi
Keyword(s):  
Western Blot ◽  
Hydatid Cyst ◽  
Larval Stage ◽  
Cystic Echinococcosis ◽  
Diagnostic Value ◽  
Cyst Fluid ◽  
Serological Tests ◽  
Blot Technique ◽  
Human Cystic Echinococcosis ◽  
Hydatid Cyst Fluid

AbstractCystic Echinococcosis (CE) is an infection caused by the larval stage of Echinococcus granulosus. The diagnosis of this disease has been problematic. Serological tests detecting antibodies against E. granulosus are the most popular and mainly use the crude Hydatid Cyst Fluid (HCF) or its components, Ag 5 and B. However, the diagnostic value of these tests is limited by the problems of specificity and/or sensitivity. The use of purified HCF antigens could be more helpful in the serodiagnosis of CE compared to the whole HCF. In the present study, we have evaluated the diagnostic value of semi-purified antigens using ELISA tests. Our results have shown that the 53 KDa antigen gave the best specificity (97.5%) and sensitivity (80%). We have also used Western Blot technique to analyze the serological profile against HCF. The results have confirmed that the most immunogenic component of HCF is the Ag 5.

scholarly journals Hydatid cyst fluid promotes peri-cystic fibrosis in cystic echinococcosis by suppressing miR-19 expression

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Chuanshan Zhang ◽  
Limin Wang ◽  
Tuergan Ali ◽  
Liang Li ◽  
Xiaojuan Bi ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Hydatid Cyst ◽  
Cystic Echinococcosis ◽  
Cyst Fluid ◽  
Hydatid Cyst Fluid

scholarly journals Usefulness of Hydatid Cyst Fluid of Echinococcus granulosus Developed in Mice with Secondary Infection for Serodiagnosis of Cystic Echinococcosis in Humans

2002 ◽  
Vol 9 (3) ◽  
pp. 573-576 ◽  
Author(s):  
Wulamu Mamuti ◽  
Hiroshi Yamasaki ◽  
Yasuhito Sako ◽  
Kazuhiro Nakaya ◽  
Minoru Nakao ◽  
...  
Keyword(s):  
Hydatid Cyst ◽  
Echinococcus Granulosus ◽  
Host Species ◽  
Cystic Echinococcosis ◽  
False Positive ◽  
Secondary Infection ◽  
Cyst Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Homogenates ◽  
Hydatid Cyst Fluid

ABSTRACT The aim of this work was to assess the usefulness of hydatid cyst fluid (HCF) of Echinococcus granulosus, obtained from mice experimentally infected with hydatid cyst tissue homogenates, for the serodiagnosis of cystic echinococcosis (CE) in humans. The sensitivity and specificity of HCF obtained from mice for the detection of immunoglobulin G (IgG) antibodies in the sera of CE patients were compared with those of HCF from sheep and/or from a human CE patient by using immunoblotting (IB) and an enzyme-linked immunosorbent assay (ELISA). HCFs obtained from three different host species all were highly useful for immunoblotting, and sera from 19 (95%) of 20 CE patients equally recognized the antigen B subunit (approximately 8 kDa). HCF from mice showed a cross-reaction with 9 of 20 alveolar echinococcosis (AE) sera (45%), whereas HCFs from two other host species cross-reacted with 14 of the AE sera (70%). Although 2 (10%) of 20 sera from neurocysticercosis (NCC) patients were false positive with HCF from both sheep and humans, none of these sera showed a positive reaction with HCF from mouse origin. ELISAs with HCFs from both mouse and sheep origins detected all 20 CE and AE sera; however, these ELISAs showed 45% (9 of 20) and 60% (12 of 20) false-positive reactions with 20 NCC sera, respectively. The presence of nonspecific human IgG in HCF obtained from a CE patient prevented us from applying it to the ELISA. HCF of E. granulosus, obtained from laboratory mice with a secondary infection with hydatid cyst tissue homogenates, appears to be highly useful for the serodiagnosis of CE in humans and may be useful in domestic animals.

scholarly journals Cross-Reaction between the Crude Hydatid Cyst Fluid Antigens of Human and Animals Origin in Response to Human IgG Class and Subclasses

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Afra Khosravi ◽  
Sobhan Ghafourian ◽  
Morteza Shamsi ◽  
Nourkhoda Sadeghifard ◽  
Abbas Maleki ◽  
...  
Keyword(s):  
Hydatid Cyst ◽  
Cross Reactivity ◽  
Cross Reaction ◽  
Cyst Fluid ◽  
Control Groups ◽  
Human Igg ◽  
Cystic Fluid ◽  
Antigen B ◽  
And Control ◽  
Hydatid Cyst Fluid

The current work aimed to evaluate the cross-reactivity of human immune sera against crude hydatid fluid antigens of sheep, human, mouse, cattle, as well as B fraction of cystic fluid antigen. 30 balb/c mice were infected with sheep hydatid cyct fluid antigen containing protoscolex after the viability of these protoscolices was assessed. ANOVA was used to test the difference of themean of optical density (OD) values among case and control groups. The highest human IgG class antibody was against antigen B (0.93) and the lowest against cattle HCF antigen (0.32). The differences between responses to these antigens were statistically significant (P<0.001). The sensitivity and specificity of ELISA test used for evaluating the responses of human total IgG to different hydatid cyst fluid (HCF) antigens among the case and control groups were 100 and 95.8%, respectively. Cross-reaction of human IgG class and subclasses responses was found almost for all the antigens with the best reaction against human and cattle (HCF) antigens and antigen B using a ratio of mean OD value to each antigen divided by the cut-off point value for the same antigen. Human sera showed a considerable cross-reactivity against all antigens by using ELISA.

scholarly journals Dot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis

2008 ◽  
Vol 50 (4) ◽  
pp. 233-236 ◽  
Author(s):  
S. R. Swarna ◽  
Subhash Chandra Parija
Keyword(s):  
Hydatid Cyst ◽  
Cyst Wall ◽  
Cystic Echinococcosis ◽  
Cyst Fluid ◽  
Healthy Controls ◽  
Blood Samples ◽  
Alternate Source ◽  
Dot Elisa ◽  
Hydatid Cyst Fluid

The aim of the present study is to evaluate cyst wall and protoscolex as an alternate source of antigen in serodiagnosis of cystic echinococcosis (CE). A total of 90 blood samples, 30 each of confirmed CE cases, disease controls and healthy controls were collected. Dot-ELISA using cyst wall, protoscolex and cyst fluid were used to demonstrate anti-hydatid antibodies. The sensitivity of Dot-ELISA using cyst wall, protoscolex and cyst fluid was 96.66%, 86.66% and 93.33% respectively and the specificity of the assay was 70% for Dot-ELISA using cyst fluid, protoscolex and cyst wall antigens. Results of the present study show that cyst wall and protoscolex can also be an useful source of antigen in detection of hydatid antibodies in the serodiagnosis of CE.

scholarly journals Immunological detection of human and camel cystic echinococcosis using different antigens of hydatid cyst fluid, protoscoleces, and germinal layers

2021 ◽  
Vol 14 (1) ◽  
pp. 270-275
Author(s):  
Mohey A. Hassanain ◽  
Nagwa I. Toaleb ◽  
Raafat M. Shaapan ◽  
Nawal A. Hassanain ◽  
Ahmed Maher ◽  
...  
Keyword(s):  
Hydatid Cyst ◽  
Cystic Echinococcosis ◽  
Clinical Signs ◽  
Liver Cyst ◽  
Cyst Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunological Study ◽  
Immunological Diagnosis ◽  
Human Sera ◽  
Hydatid Cyst Fluid

Background and Aim: Cystic echinococcosis (CE)/hydatidosis is one of the most prevalent neglected zoonotic diseases. It is initially asymptomatic and does not produce any clinical signs until the cyst becomes enlarged, causing localized pressure on internal organs and tissues. Therefore, the detection of Echinococcus granulosus antibodies is highly essential. This study evaluated the antigens of hydatid cyst fluid, protoscoleces, and germinal layers for efficient immunological diagnosis of CE in humans and camels. Materials and Methods: Hydatid cyst fluid (FLc), protoscoleces (Psc), and the germinal layer (GLc) antigens were prepared from camel-lung hydatid cysts. In the same way, hydatid cyst fluid (FLh) and protoscoleces (Psh) antigens from human-liver cyst aspirate were produced. The comparative immunodiagnostic efficacy of the prepared antigens was verified using indirect enzyme-linked immunosorbent assay (ELISA), SDS-PAGE, and immunoblotting. Results: ELISA proves that FLc and GLc antigens were higher than FLh and Psh antigens. This shows that binding reactivity in naturally infected human sera, camel sera, and Psc is the most potent, exhibiting 100% sensitivity with 78.26% and 76.47% specificity in camel and human sera, respectively. The CE prevalence using diagnostic Psc was 54.79% and 61.32% in tested human and camel sera, respectively. The electrophoretic profiles of all shared antigens showed similarities at 52, 41, and 22 kDa. Immunoblotting demonstrated common immune-reactive bands in all antigen types at 52 and 41 kDa against positive human and camel sera. Conclusion: This immunological study introduces camel hydatid cyst Psc as a potent diagnostic antigen and new immune-reactive fractions of 52 and 41 kDa for diagnosing hydatidosis in humans and camels.

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