Visceral and post-Kala-Azar dermal leishmaniasis isolates show significant difference in their in vitro drug susceptibility pattern

In this study, our aim was to determine the predominant genotypes among the Mycobacterium tuberculosis (MTB) strains circulating in Zhejiang Province. In addition, we also sought to determine the potential associations between MTB genotypes and susceptibility to first-line drugs. Out of these isolates, 673 (71.6%) were classified into the Beijing genotype, while the other 267 (28.4%) were from non-Beijing families. The highest proportion of Beijing genotype was found in Huzhou (80.0%) and the lowest in Lishui (48.3%). Statistical analysis revealed that there was a significant difference in the prevalence of Beijing genotype among different regions (χ2 = 17.57, P = 0.04). In addition, the overall proportions of drug resistance to INH, RIF, SM, and EMB were 13.2% (124/940), 21.8% (75/940), 3.4% (32/940), and 5.9% (55/940) in Zhejiang, respectively. Further comparison revealed that there was no significant difference in drug susceptibility profiles between Beijing and non-Beijing strains (P > 0.05). In conclusion, we describe the genetic diversity and drug susceptibility pattern of MTB in Zhejiang for the first time. Our data demonstrate that Beijing genotype is the predominant lineage in Zhejiang, while the distribution of Beijing-genotype strains shows geographic diversity. In addition, no correlation is observed between Beijing genotype and anti-TB drug resistance.

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Group B streptococci vaginal colonization and drug susceptibility pattern among pregnant women attending in selected public antenatal care centers in Addis Ababa, Ethiopia

BMC Pregnancy and Childbirth ◽

10.1186/s12884-018-1791-4 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 3

Author(s):

Solomon Assefa ◽

Kassu Desta ◽

Tsehaynesh Lema

Keyword(s):

Antenatal Care ◽

Pregnant Women ◽

Addis Ababa ◽

Drug Susceptibility ◽

Susceptibility Pattern ◽

Group B Streptococci ◽

Vaginal Colonization ◽

Drug Susceptibility Pattern ◽

Group B

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Growth, Drug Susceptibility, and Gene Expression Profiling of Plasmodium falciparum Cultured in Medium Supplemented with Human Serum

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057107310638 ◽

2007 ◽

Vol 12 (8) ◽

pp. 1109-1114 ◽

Cited By ~ 14

Author(s):

Kshipra Singh ◽

Ameeta Agarwal ◽

Shabana I. Khan ◽

Larry A. Walker ◽

Babu L. Tekwani

Keyword(s):

Gene Expression ◽

Plasmodium Falciparum ◽

Human Serum ◽

Global Gene Expression ◽

Drug Susceptibility ◽

Growth Cycle ◽

In Vitro Cultivation ◽

Human Malaria Parasite ◽

In Vitro Drug Susceptibility

In vitro cultivation of Plasmodium falciparum has been extremely useful in understanding the biology of the human malaria parasite as well as research on the discovery of new antimalarial drugs and vaccines. A chemically defined serum-free medium supplemented with lipid-rich bovine serum albumin (AlbuMAX I) offers the following advantages over human serum-supplemented media for the in vitro culture of P. falciparum: 1) improved growth profile, with more than a 2-fold higher yield of the parasites at any stage of the growth cycle; 2) suitability for in vitro antimalarial screening, as the parasites grown in AlbuMAX and human serum-supplemented media show similar sensitivity to standard and novel antimalarials as well as natural product extracts in the in vitro drug susceptibility assays; and 3) DNA microarray analysis comparing the global gene expression profile of sorbitol-synchronized P. falciparum trophozoites grown in the 2 different media, indicating minimal differences. ( Journal of Biomolecular Screening 2007:1109-1114)

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