Ectopic expression of sex-peptide in a variety of tissues in Drosophila females using the P[GAL4] enhancer-trap system

1997 ◽  
Vol 254 (4) ◽  
pp. 449-455 ◽  
Author(s):  
S. Nakayama ◽  
K. Kaiser ◽  
T. Aigaki
Keyword(s):  
Ectopic Expression ◽  
Enhancer Trap ◽  
Sex Peptide ◽  
Trap System

Establishment of an enhancer trap system with Ds and GUS for functional genomics in rice

2004 ◽  
Vol 271 (6) ◽  
pp. 639-650 ◽  
Author(s):  
Y. Ito ◽  
M. Eiguchi ◽  
N. Kurata
Keyword(s):  
Functional Genomics ◽  
Enhancer Trap ◽  
Trap System

scholarly journals Combination of the ALCR/alcA ethanol switch and GAL4/VP16-UAS enhancer trap system enables spatial and temporal control of transgene expression in Arabidopsis

2007 ◽  
Vol 5 (4) ◽  
pp. 477-482 ◽  
Author(s):  
Hongge Jia ◽  
Bram Van Loock ◽  
Mingjun Liao ◽  
Jean-Pierre Verbelen ◽  
Kris Vissenberg
Keyword(s):  
Transgene Expression ◽  
Enhancer Trap ◽  
Temporal Control ◽  
Trap System

Ectopic expression of sex peptide alters reproductive behavior of female D. melanogaster

Neuron ◽  
1991 ◽  
Vol 7 (4) ◽  
pp. 557-563 ◽  
Author(s):  
Toshiro Aigaki ◽  
Iréne Fleischmann ◽  
Pei-Shen Chen ◽  
Eric Kubli
Keyword(s):  
Reproductive Behavior ◽  
Ectopic Expression ◽  
Sex Peptide

Construction of a piggyBac-based enhancer trap system for the analysis of gene function in silkworm Bombyx mori

2008 ◽  
Vol 38 (12) ◽  
pp. 1165-1173 ◽  
Author(s):  
Keiro Uchino ◽  
Hideki Sezutsu ◽  
Morikazu Imamura ◽  
Isao Kobayashi ◽  
Ken-Ichiro Tatematsu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Gene Function ◽  
Enhancer Trap ◽  
Trap System ◽  
Silkworm Bombyx Mori

scholarly journals Development of GAL4/VP16 Facilitated-enhancer Trap System for Rice Crop Improvement

2009 ◽  
Vol 5 (1) ◽  
pp. 16
Author(s):  
Sri Kurniati ◽  
Andrzej Kilian
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Southern Blot ◽  
Crop Improvement ◽  
Enhancer Trap ◽  
Rice Crop ◽  
Trap System

<p>Pengembangan Sistem Perangkap Enhancer yang Difasilitasi<br />oleh Aktivator Transkripsi GAL4/VP16 untuk Perbaikan<br />Tanaman Padi. Sri Koerniati dan Andrzej Kilian.<br />Peningkatan produksi padi untuk memenuhi kebutuhan<br />nasional dilakukan melalui perbaikan tanaman, termasuk<br />pencarian dan isolasi gen-gen baru melalui mutasi. Aplikasi<br />berbagai sekuen mutagen, elemen loncat atau T-DNA, didukung<br />dengan teknik PCR merupakan perdekatan yang lebih<br />baik dibandingkan dengan metode klasik. Perangkap enhancer<br />adalah sistem yang dikembangkan untuk mengatasi<br />masalah rendahnya tingkat perolehan mutan, akibat banyak<br />gen berfungsi sama atau satu gen berfungsi pada beberapa<br />tingkat perkembangan tanaman. Sistem ini mampu menampilkan<br />ekspresi pada jaringan tertentu (spatial) dan atau<br />pada waktu tertentu (temporal) pada tanaman hemizigot<br />(hemizygous plants). Penelitian ini bertujuan untuk (1) mengembangkan<br />vector cassette perangkap enhancer dengan<br />komponen utama aktivator transkripsi GAL4/VP16 dan dua<br />gen reporter (gus dan gusPlus), dan (2) memperoleh informasi<br />tentang ekspresinya pada padi. Sepuluh vector diperoleh<br />dan ditransformasikan ke kalus padi Nipponbare dan<br />Millin dengan Agrobacterium tumefaciens. Kajian vektor<br />melalui ekspresi gen reporter diamati pada kalus 3 hari<br />setelah co-cultivation dan jaringan vegetatif dari 745 lini<br />penangkap enhancer. Sembilan puluh lima persen nomor<br />memiliki ekspresi dan persentase lebih tinggi daripada yang<br />telah dilaporkan sebelumnya. Lini dengan vektor GAL4/VP16<br />delesi tidak memiliki ekspresi pada kalus dan jaringan vegetatif,<br />walaupun hasil Southern Blot menunjukkan tanaman<br />ini memiliki dua T-DNA. Tiga puluh dua persen lini gusPlus<br />memiliki ekspresi yang kuat, sedangkan 30% berekspresi<br />lemah dibandingkan dengan masing-masing 12% dan 47%<br />untuk lini gus. Lini gusPlus juga tersebar pada lebih banyak<br />pola ekspresi. Jumlah insersi pada lini perangkap enhancer<br />berkisar antara 1-7 T-DNA dan 49% di antaranya memiliki<br />satu T-DNA. gusPlus merupakan gen reporter yang lebih<br />sensitif daripada gus dan GAL4/VP16 terbukti berfungsi.</p>

The Prognostic and Predictive Value of microRNAs in Patients with H. pylori-positive Gastric Cancer

2019 ◽  
Vol 24 (39) ◽  
pp. 4639-4645 ◽  
Author(s):  
Seyed Mostafa Parizadeh ◽  
Reza Jafarzadeh-Esfehani ◽  
Amir Avan ◽  
Maryam Ghandehari ◽  
Fatemeh Goldani ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Suppressors ◽  
Ectopic Expression ◽  
Noncoding Rnas ◽  
World Population ◽  
Normal Flora ◽  
Small Noncoding Rnas ◽  
Control Gene Expression ◽  
The World ◽  
H Pylori

Gastric cancer (GC) has a high mortality rate with a poor 5-year survival. Helicobacter pylori (H. pylori) is present as part of the normal flora of stomach. It is found in the gastric mucosa of more than half of the world population. This bacterium is involved in developing H. pylori-induced GC due to the regulation of different micro ribonucleic acid (miRNA or miR). miRNAs are small noncoding RNAs and are recognized as prognostic biomarkers for GC that may control gene expression. miRNAs may function as tumor suppressors, or oncogenes. In this review, we evaluated studies that investigated the ectopic expression of miRNAs in the prognosis of H. pylori positive and negative GC.

Efficient AAV-mediated Gene Targeting Using 2A-based Promoter-trap System

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (24) ◽  
Author(s):  
Sivasundaram Karnan ◽  
Akinobu Ota ◽  
Yuko Konishi ◽  
Md Wahiduzzaman ◽  
Shinobu Tsuzuki ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Trap System ◽  
Promoter Trap

scholarly journals TEAD4 modulated LncRNA MNX1-AS1 contributes to gastric cancer progression partly through suppressing BTG2 and activating BCL2

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
You Shuai ◽  
Zhonghua Ma ◽  
Weitao Liu ◽  
Tao Yu ◽  
Changsheng Yan ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Progression ◽  
Molecular Mechanisms ◽  
Mechanistic Model ◽  
Ectopic Expression ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Tissue Samples ◽  
Reporter Assays

Abstract Background Gastric cancer (GC) is the third leading cause of cancer-related mortality globally. Long noncoding RNAs (lncRNAs) are dysregulated in obvious malignancies including GC and exploring the regulatory mechanisms underlying their expression is an attractive research area. However, these molecular mechanisms require further clarification, especially upstream mechanisms. Methods LncRNA MNX1-AS1 expression in GC tissue samples was investigated via microarray analysis and further determined in a cohort of GC tissues via quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. Cell proliferation and flow cytometry assays were performed to confirm the roles of MNX1-AS1 in GC proliferation, cell cycle regulation, and apoptosis. The influence of MNX1-AS1 on GC cell migration and invasion was explored with Transwell assays. A xenograft tumour model was established to verify the effects of MNX1-AS1 on in vivo tumourigenesis. The TEAD4-involved upstream regulatory mechanism of MNX1-AS1 was explored through ChIP and luciferase reporter assays. The mechanistic model of MNX1-AS1 in regulating gene expression was further detected by subcellular fractionation, FISH, RIP, ChIP and luciferase reporter assays. Results It was found that MNX1-AS1 displayed obvious upregulation in GC tissue samples and cell lines, and ectopic expression of MNX1-AS1 predicted poor clinical outcomes for patients with GC. Overexpressed MNX1-AS1 expression promoted proliferation, migration and invasion of GC cells markedly, whereas decreased MNX1-AS1 expression elicited the opposite effects. Consistent with the in vitro results, MNX1-AS1 depletion effectively inhibited the growth of xenograft tumour in vivo. Mechanistically, TEAD4 directly bound the promoter region of MNX1-AS1 and stimulated the transcription of MNX1-AS1. Furthermore, MNX1-AS1 can sponge miR-6785-5p to upregulate the expression of BCL2 in GC cells. Meanwhile, MNX1-AS1 suppressed the transcription of BTG2 by recruiting polycomb repressive complex 2 to BTG2 promoter regions. Conclusions Our findings demonstrate that MNX1-AS1 may be able to serve as a prognostic indicator in GC patients and that TEAD4-activatd MNX1-AS1 can promote GC progression through EZH2/BTG2 and miR-6785-5p/BCL2 axes, implicating it as a novel and potent target for the treatment of GC.

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