Metabolic flux and robustness analysis of glycerol metabolism in Klebsiella pneumoniae

2007 ◽  
Vol 31 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Qingrui Zhang ◽  
Hu Teng ◽  
Yaqin Sun ◽  
Zhilong Xiu ◽  
Anping Zeng
Keyword(s):  
Klebsiella Pneumoniae ◽  
Metabolic Flux ◽  
Robustness Analysis ◽  
Glycerol Metabolism

scholarly journals Rewiring of glycerol metabolism in Escherichia coli for effective production of recombinant proteins

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Chung-Jen Chiang ◽  
Yi-Jing Ho ◽  
Mu-Chen Hu ◽  
Yun-Peng Chao
Keyword(s):  
Escherichia Coli ◽  
Crude Glycerol ◽  
Metabolic Flux ◽  
Raw Materials ◽  
Tricarboxylic Acid Cycle ◽  
Cost Effective ◽  
Fold Increase ◽  
Pentose Phosphate ◽  
Glycerol Metabolism ◽  
Related Field

Abstract Background The economic viability of a protein-production process relies highly on the production titer and the price of raw materials. Crude glycerol coming from the production of biodiesel is a renewable and cost-effective resource. However, glycerol is inefficiently utilized by Escherichia coli. Results This issue was addressed by rewiring glycerol metabolism for redistribution of the metabolic flux. Key steps in central metabolism involving the glycerol dissimilation pathway, the pentose phosphate pathway, and the tricarboxylic acid cycle were pinpointed and manipulated to provide precursor metabolites and energy. As a result, the engineered E. coli strain displayed a 9- and 30-fold increase in utilization of crude glycerol and production of the target protein, respectively. Conclusions The result indicates that the present method of metabolic engineering is useful and straightforward for efficient adjustment of the flux distribution in glycerol metabolism. The practical application of this methodology in biorefinery and the related field would be acknowledged.

Cell physiology and metabolic flux response of Klebsiella pneumoniae to aerobic conditions

2009 ◽  
Vol 44 (8) ◽  
pp. 862-868 ◽  
Author(s):  
Zhen Chen ◽  
Hong-Juan Liu ◽  
Jian-An Zhang ◽  
De-Hua Liu
Keyword(s):  
Klebsiella Pneumoniae ◽  
Metabolic Flux ◽  
Cell Physiology ◽  
Aerobic Conditions ◽  
Flux Response

Glycerol metabolism of Pichia pastoris (Komagataella spp.) characterised by 13C-based metabolic flux analysis

2019 ◽  
Vol 50 ◽  
pp. 52-59 ◽  
Author(s):  
Màrius Tomàs-Gamisans ◽  
Anders Sebastian Rosenkrans Ødum ◽  
Mhairi Workman ◽  
Pau Ferrer ◽  
Joan Albiol
Keyword(s):  
Pichia Pastoris ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Glycerol Metabolism ◽  
Flux Analysis

Metabolic flux change in Klebsiella pneumoniae L17 by anaerobic respiration in microbial fuel cell

2016 ◽  
Vol 21 (2) ◽  
pp. 250-260 ◽  
Author(s):  
Changman Kim ◽  
Satish Kumar Ainala ◽  
You-Kwan Oh ◽  
Byong-Hun Jeon ◽  
Sunghoon Park ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Klebsiella Pneumoniae ◽  
Microbial Fuel Cell ◽  
Metabolic Flux ◽  
Anaerobic Respiration ◽  
Flux Change
Sign in / Sign up

Export Citation Format

Share Document