Isobutanol production in engineered Saccharomyces cerevisiae by overexpression of 2-ketoisovalerate decarboxylase and valine biosynthetic enzymes

2012 ◽  
Vol 35 (9) ◽  
pp. 1467-1475 ◽  
Author(s):  
Won-Heong Lee ◽  
Seung-Oh Seo ◽  
Yi-Hyun Bae ◽  
Hong Nan ◽  
Yong-Su Jin ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Biosynthetic Enzymes ◽  
Engineered Saccharomyces Cerevisiae

Production of (–)-α-bisabolol in metabolically engineered Saccharomyces cerevisiae

2021 ◽  
Author(s):  
Tae Yeob Kim ◽  
Haeseong Park ◽  
Sun-Ki Kim ◽  
Soo-Jung Kim ◽  
Yong-Cheol Park
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Engineered Saccharomyces Cerevisiae

Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae

2018 ◽  
Vol 103 (3) ◽  
pp. 1243-1254 ◽  
Author(s):  
Jyumpei Kobayashi ◽  
Daisuke Sasaki ◽  
Takahiro Bamba ◽  
Tomohisa Hasunuma ◽  
Akihiko Kondo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sustainable Production ◽  
Engineered Saccharomyces Cerevisiae

scholarly journals Establishment of Strigolactone-Producing Bacterium-Yeast Consortium

2021 ◽  
Author(s):  
Sheng Wu ◽  
Xiaoqiang Ma ◽  
Anqi Zhou ◽  
Alex Valenzuela ◽  
Yanran Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Plant Growth ◽  
Production Process ◽  
Growth And Development ◽  
Pathway Engineering ◽  
Biosynthetic Enzymes ◽  
Functional Identification ◽  
Plant Growth And Development ◽  
Microbial System

Strigolactones (SLs) are a class of phytohormones playing diverse roles in plant growth and development, yet the limited access to SLs is largely impeding SL-based foundational investigations and applications. Here, we developed Escherichia coli-Saccharomyces cerevisiae consortia to establish a microbial biosynthetic platform for the synthesis of various SLs, including carlactone, carlactonic acid, 5-deoxystrigol (5DS), 4-deoxyorobanchol (4DO), and orobanchol (OB). The SL-producing platform enabled us to conduct functional identification of CYP722Cs from various plants as either OB or 5DS synthase. It also allowed us to quantitatively compare known variants of plant SL biosynthetic enzymes in the microbial system. The titer of 5DS was further enhanced through pathway engineering to 0.0473 mg/L. This work provides a unique platform for investigating SL biosynthesis and evolution and lays the foundation for developing SL microbial production process.

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