scholarly journals The impact of technical failures on recombinant production of soluble proteins in Escherichia coli: a case study on process and protein robustness

2021 ◽  
Author(s):  
Alexander Pekarsky ◽  
Melanie Reininger ◽  
Oliver Spadiut
Keyword(s):  
Protein Quality ◽  
Ph Control ◽  
Cell Physiology ◽  
E Coli ◽  
Recombinant Production ◽  
Regeneration Phase ◽  
The Impact ◽  
Technical Failures

AbstractTechnical failures lead to deviations in process parameters that can exceed studied process boundaries. The impact on cell and target protein is often unknown. However, investigations on common technical failures might yield interesting insights into process and protein robustness. Recently, we published a study on the impact of technical failures on an inclusion body process that showed high robustness due to the inherent stability of IBs. In this follow-up study, we investigated the influence of technical failures during production of two soluble, cytosolic proteins in E. coli BL21(DE3). Cell physiology, productivity and protein quality were analyzed, after technical failures in aeration, substrate supply, temperature and pH control had been triggered. In most cases, cell physiology and productivity recovered during a subsequent regeneration phase. However, our results highlight that some technical failures lead to persistent deviations and affect the quality of purified protein.

Technology and Hospital Information System: A Case of Implementation of Electronic Clinical Records in Hospital Maternity Irene Neto in Lubango, Angola

2019 ◽  
Vol 10 (11) ◽  
pp. 1131-1135
Author(s):  
Tomas Hambili Paulo Sanjuluca ◽  
◽  
Ricardo Correia ◽  
Anabela Antunes de Almeida ◽  
Ana Gloria Diaz Martinez ◽  
...  
Keyword(s):  
Maternity Hospital ◽  
Management Support ◽  
Retrospective Case ◽  
System A ◽  
Clinical Records ◽  
Retrospective Case Study ◽  
The Impact ◽  
And Child Health

Introduction: In order to have a good assessment of the quality of maternal and child health care, it is essential that there is up-to-date and reliable information. Objective: To evaluate the impact of the implementation of a computerized database of clinical processes in the admission, archive and medical statistics section, of Maternity hospital Irene Neto/Lubango-Angola. Methodology: A descriptive study with a quantitative and qualitative approach to carry out a retrospective case study deliveries and newborns, records from 2014 to 2017. Final considerations: The implementation of this project may contribute to the improvement of clinical management support management of the hospital as well as facilitating access to information for research and scientific production.

Impact of Raw Materials and Processing Techniques on The Microbiological Quality of Egyptian Domiati Cheese

2020 ◽  
Keyword(s):  
Raw Materials ◽  
Microbiological Quality ◽  
Raw Milk ◽  
Plate Count ◽  
E Coli ◽  
Fresh Cheese ◽  
Processing Techniques ◽  
The Impact ◽  
Total Yeast

Domiati cheese is the most popular brand of cheese ripened in brine in the Middle East in terms of consumed quantities. This study was performed to investigate the impact of the microbiological quality of the used raw materials, the applied traditional processing techniques and ripening period on the quality and safety of the produced cheese. Three hundred random composite samples were collected from three factories at Fayoum Governorate, Egypt. Collected samples represent twenty-five each of: raw milk, table salt, calf rennet, microbial rennet, water, environmental air, whey, fresh cheese, ripened cheese & swabs from: worker hands; cheese molds and utensils; tanks. All samples were examined microbiologically for Standard Plate Count (SPC), coliforms count, Staphylococcus aureus (S. aureus) count, total yeast & mould count, presence of E. coli, Salmonellae and Listeria monocytogenes (L. monocytogenes). The mean value of SPC, coliforms, S. aureus and total yeast & mould counts ranged from (79×102 CFU/m3 for air to 13×108 CFU/g for fresh cheese), (7×102 MPN/ cm2 for tank swabs to 80×106 MPN/ml for raw milk), (9×102 CFU/g for salt to 69×106 CFU/g for fresh cheese) and (2×102 CFU/cm2 for hand swabs to 60×104 CFU/g for fresh cheese), respectively. Whereas, E. coli, Salmonella and L. monocytogenes failed to be detected in all examined samples. There were significant differences in all determined microbiological parameters (p ≤0.05) between fresh and ripened cheese which may be attributed to different adverse conditions such as water activity, pH, salt content and temperature carried out to improve the quality of the product.

scholarly journals Impact of treated sewage on meiobenthic nematodes: a case study from the Tunisian Refining Industries Company

2022 ◽  
Vol 82 ◽  
Author(s):  
A. Hedfi ◽  
M. Ben Ali ◽  
A. Noureldeen ◽  
H. Darwish ◽  
T. Saif ◽  
...  
Keyword(s):  
Organic Matter ◽  
Environmental Parameters ◽  
The Other ◽  
Treated Sewage ◽  
Before And After ◽  
Bizerte Bay ◽  
De Man ◽  
The Impact

Abstract The main objective of the current study was to assess the impact of the water taken from the ‘Tunisian Refining Industries Company’ on meiobenthic nematodes, before and after a series of treatments in decantation basins followed by its discharge in Bizerte bay, Tunisia. The comparison of environmental parameters of the two types of water was clearly indicative of an improvement in the quality of treated waters after a significant reduction in their loads in hydrocarbons. Overall, the water retained a good quality after being treated by ‘Tunisian Refining Industries Company’ before discharge in the sea. At the end of the experiment, differential responses were observed according to the richness of sediment in organic matter and hydrocarbons. Thus, it was apparent that the nematode assemblage exposed to the treated waters was closer to controls and associated to higher values of abundance, than that under untreated ones. It was also assumed that the species Microlaimus honestus De Man, 1922, Paramonohystera proteus Wieser, 1956 and Cyartonema germanicum Juario, 1972 are sensitive bioindicators of bad environmental statues and of hydrocarbon presence in the environment. On the other hand, Metoncholaimus pristiurus (Zur Strassen, 1894) Filipjev, 1918 would rather be classified as a positive bioindicative species of this type of pollutants.

scholarly journals The impact of coal ash and slag dump on the quality of surface and ground waters - a case study

2019 ◽  
Vol 84 (5) ◽  
pp. 527-530
Author(s):  
Ivana Cipranic ◽  
Radmila Markovic ◽  
Stefan Djordjievski ◽  
Zoran Stevanovic ◽  
Marija Stevanovic
Keyword(s):  
Water Samples ◽  
Coal Ash ◽  
Negative Influence ◽  
Physical Treatment ◽  
Physical Chemical ◽  
Quality Of Water ◽  
Slag Dump ◽  
The Impact

This paper presents the assessment of the impact of coal ash and slag from the ?Maljevac? dump on the quality of water of Paleski Creek, Montenegro. The obtained results confirm the negative influence of surface and groundwater from the dump on the water from the Paleski Creek. The results obtained by testing of the water samples, collected from Paleski Creek upstream of the dump, indicate that the water is qualified to be used for drinking, after simple physical treatment and disinfection. The results obtained for the water samples collected from the Paleski creek downstream from the dump indicate that the water could be safe for drinking only after the treatment that requires an intensive physical, chemical and biological processing, including some extended treatment.

scholarly journals Analysis of The Impact of Industrial Activities Towards River Vacancy Viewed from BODS, CODS, and TSS Using The QUAL2KW Software Method Approach (Case Study: Klampok River, Semarang District)

2020 ◽  
Vol 202 ◽  
pp. 04008
Author(s):  
Nurandani Hardyanti ◽  
Winardi D Nugraha ◽  
Vito Edgar S B
Keyword(s):  
Water Quality ◽  
Surface Water ◽  
River Water ◽  
Catchment Area ◽  
Industrial Sector ◽  
Industrial Activities ◽  
The Impact ◽  
Method Approach

The industrial sector is one of the important sector in supporting the development of a region. Utilization of land around the river that is used for industrial activities will affect the quality of river water. The river can be polluted by waste personinating from industries that operating around the river. The catchment area that used for industry, agriculture, urban development, and the use of land for making roads (gravel or footpaths) can affect the flow of surface water and sediments that it brings to the river. Waste generated from industrial activities can pollute rivers which are a source of water for daily needs and affect the development of biota in them. This can affect river water quality

scholarly journals The Impact of Selected Determinants on the Perceived Competitiveness and Quality of Processor Manufacturers: A Case Study

2021 ◽  
Vol XXIV (Issue 1) ◽  
pp. 954-983
Author(s):  
Agnieszka Barcik ◽  
Piotr Dziwinski ◽  
Jozef Ledzianowski ◽  
Justyna Malysiak
Keyword(s):  
The Impact
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