KINETIC STUDY COMPARISON OF IMMOBILIZED GLUCOSE ISOMERASE (GENSWEET AND SWEETZYME IT) IN STIRRED TANK REACTOR AND PACKED BED REACTOR

D- Glucose/xylose isomerase catalysis the reversible isomerization of aldoses to ketoses such as D-glucose and D-xylose to D-fructose and D-xylose respectively. High fructose corn syrup (HFCS), a low calorie sugar substitute for cane sugar, utilizes Glucose isomerase enzyme for conversion of glucose to fructose. The conversion of glucose to fructose favours more at high temperature, providing an incentive to utilize thermostable and thermoactive glucose isomerase in High fructose corn syrup (HFCS) production. Present studies emphasize on enzymatic conversion and optimization using Sweetzyme IT extra & Gensweet, commercially available glucose isomerases. The experiments were carried out for enzymatic conversion of glucose to fructose using Gensweet and Sweetzyme in Packed bed reactor (PBR) and Stirred tank reactor (STR). Maximum conversion was seen in Stirred tank reactor (STR) using both of these enzymes, approx 10 % more Fructose conversion comparing it to packed bed reactor (PBR). Also, Stirred tank reactor (STR) reaction conditions such as pH, buffers, cofactor (MgSO4) requirement were optimized to achieve optimum enzyme activity. Analysis of enzymatic conversion samples was done using HPLC-RID (using Zorbax Column). The importance of the divalent cation MgCl2 for optimal enzyme activity was investigated. The enzyme performed best at pH 7.5 and 60°C, using 10mM MgSO4 as a cofactor. Utilizing Gensweet in Stirred tank reactor (STR), the maximum fructose transformation was 44 %. The most activity was detected with Sodium phosphate buffers, and EPPS buffers at pH 7 and 8, accordingly, whereas the least activity was reported with TRIS HCl buffer.

Download Full-text

Evaluation of Packed-Bed Reactor and Continuous Stirred Tank Reactor for the Production of Colchicine Derivatives

ISRN Chemical Engineering ◽

10.1155/2013/865618 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Kashyap Kumar Dubey ◽

Dhirendra Kumar ◽

Punit Kumar ◽

Shafiul Haque ◽

Arshad Jawed

Keyword(s):

Optimization Technique ◽

Packed Bed ◽

Packed Bed Reactor ◽

Stirred Tank ◽

Process Time ◽

Continuous Stirred Tank Reactor ◽

Stirred Tank Reactor ◽

Tank Reactor ◽

Continuous Stirred Tank ◽

Pharmacologically Active

Bioconversion of colchicine into its pharmacologically active derivative 3-demethylated colchicine (3-DMC) mediated by P450BM3 enzyme is an economic and promising strategy for the production of this inexpensive and potent anticancer drug. Continuous stirred tank reactor (CSTR) and packed-bed reactor (PBR) of 3 L and 2 L total volumes were compared for the production of 3-demethylated colchicine (3-DMC) a colchicine derivative using Bacillus megaterium MTCC*420 under aerobic conditions. Statistical optimization technique was utilized with the most significant variables, that is, dissolved oxygen (DO), colchicine concentration, and process time for optimization. The validation of the model was performed by experiments on the predicted values in an individual run, and the optimum parameters were DO (~50%), colchicine concentration (7.5 g/L), and process time (39 h) resulted in a maximum bioconversion of 3-DMC 3.36 g/L. The PBR reactor achieved much higher productivity (6.58 g/L/h) as reported by earlier researchers. This is the first report on the use of PBR for bioconversion of colchicine.

Download Full-text

Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high fructose corn syrup in packed bed reactor

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-021-02560-4 ◽

2021 ◽

Author(s):

Li-Qun Jin ◽

Xian-Xiao Chen ◽

Yi-Ting Jin ◽

Jun-Kang Shentu ◽

Zhi-Qiang Liu ◽

...

Keyword(s):

Escherichia Coli ◽

Packed Bed ◽

Glucose Isomerase ◽

Packed Bed Reactor ◽

Recombinant Escherichia Coli ◽

High Fructose Corn Syrup ◽

Corn Syrup ◽

Escherichia Coli Cells ◽

High Fructose ◽

Modified Diatomite

Download Full-text

A comparative study of immobilized amyloglucosidase in a packed bed reactor and a continuous feed stirred tank reactor

Biotechnology and Bioengineering ◽

10.1002/bit.260130302 ◽

1971 ◽

Vol 13 (3) ◽

pp. 337-352 ◽

Cited By ~ 93

Author(s):

S. P. O'Neill ◽

P. Dunnill ◽

M. D. Lilly

Keyword(s):

Comparative Study ◽

Packed Bed ◽

Packed Bed Reactor ◽

Stirred Tank ◽

Stirred Tank Reactor ◽

Tank Reactor ◽

Continuous Feed

Download Full-text

Ozonation of CI Reactive Black 5 using rotating packed bed and stirred tank reactor

Journal of Chemical Technology & Biotechnology ◽

10.1002/jctb.1159 ◽

2004 ◽

Vol 80 (1) ◽

pp. 68-75 ◽

Cited By ~ 34

Author(s):

Yi-Hung Chen ◽

Ching-Yuan Chang ◽

Wei-Ling Su ◽

Chun-Yu Chiu ◽

Yue-Hwa Yu ◽

...

Keyword(s):

Packed Bed ◽

Stirred Tank ◽

Reactive Black 5 ◽

Stirred Tank Reactor ◽

Tank Reactor ◽

Rotating Packed Bed

Download Full-text

Conceptual Study of Transesterification of Vegetable Oils in the Continuous-Stirred-Tank Reactor at Unsteady-State and Isothermal Conditions

Malaysian Journal of Fundamental and Applied Sciences ◽

10.11113/mjfas.v17n2.2006 ◽

2021 ◽

Vol 17 (2) ◽

pp. 181-194

Author(s):

Fadzil Noor Gonawan ◽

Azlina Harun Kamaruddin

Keyword(s):

Flow Rate ◽

Enzymatic Reaction ◽

Volumetric Flow Rate ◽

Packed Bed ◽

Stirred Tank ◽

Continuous Stirred Tank Reactor ◽

Stirred Tank Reactor ◽

Tank Reactor ◽

Isothermal Conditions ◽

Continuous Stirred Tank

The continuous-stirred-tank reactor (CSTR) is favorable for bi-phasic enzymatic reaction due to ease of operation, cost-effective and low downtime. Lack of study on the enzymatic reaction in the CSTR has disfavor this type of reactor compared to batch and packed bed. Presently, a simulation was carried out to simulate the behavior of the lipase-catalyzed production of biodiesel by using CSTR at isothermal conditions. The mathematical model incorporated the effect of the kinetic, thermal, and operating parameters. The parameters such as Michaelis constant (Km), inhibition constant (Ki), Gibbs inactivation energy (DelG) and mol flow rate are among determining factors of the course of the reaction. It is suggested that the enzyme with lower , higher , and higher should be chosen for the reaction. In continuous operation in the CSTR, the volumetric flow rate of the substrates and the initial concentration of the feed could be used to control reaction performance as these parameters will determine the total mol or ratio of the substrates in the reactor. Most, importantly, the longer residence time is preferred to achieve higher conversion, however, the volumetric flow rate must not be too low to prevent underperformance of reaction.

Download Full-text