Systemic and peritoneal inflammatory response after laparoscopic-assisted gastrectomy and the effect of inflammatory cytokines on adhesion of gastric cancer cells to peritoneal mesothelial cells

2010 ◽  
Vol 24 (11) ◽  
pp. 2860-2870 ◽  
Author(s):  
Ge Yu ◽  
Bo Tang ◽  
Pei-Wu Yu ◽  
Zhi-hong Peng ◽  
Feng Qian ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Inflammatory Response ◽  
Cancer Cells ◽  
Inflammatory Cytokines ◽  
Mesothelial Cells ◽  
Gastric Cancer Cells ◽  
Peritoneal Mesothelial Cells ◽  
Laparoscopic Assisted

The regulation of stem-like property in gastric cancer cells mediated by tumor associated macrophage.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e13553-e13553
Author(s):  
Takatsugu Ishimoto ◽  
Masayuki Watanabe ◽  
Masaaki Iwatsuki ◽  
Satoshi Ida ◽  
Yohei Nagai ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Inflammatory Cytokines ◽  
Functional Role ◽  
M2 Macrophage ◽  
Human Gastric Cancer ◽  
Gastric Cancer Cells ◽  
Tumor Associated Macrophage ◽  
Bmi1 Expression ◽  
Activated Macrophage

e13553 Background: CD44 has recently been identified as one of the cell surface markers associated with cancer stem cells (CSCs) in several types of tumor. We have reported the functional role of CD44 variant isoform in the maintenance of low reactive oxygen species (ROS) levels in gastrointestinal cancer cells. Previous studies demonstrated that tumor associated macrophage promotes tumor progression, but the functional role for macrophage in the regulation of CSC marker expression is not known. Methods: We analysed the relationship of CSCs and macrophage infiltration in gastric cancer cell lines and human gastric cancer samples. Results: Here, we showed activated macrophage by LPS and IFNγ produces inflammatory cytokines and chemokines, and these macrophage express CD68 and CD163, which are known as specific markers for pan-macrophage and M2 macrophage. we found that co-culture with activated macrophage triggers the CD44 and Bmi1 expression in gastric cancer cells and promote stem-like property that possess sphere-forming ability. Furthermore, we investigated the relation between CD44 expression and infiltrated macrophage in human gastric cancer. The staining patterns of CD44, Bmi1 and macrophage specific markers (CD68 and CD163) were significantly correlated in tumorous tissues of human gastric adenocarcinoma. Conclusions: These findings establish inflammatory cytokines, which producted from activated macrophage, induce the CD44 and Bmi1 expression in gastric cancer cells. These findings revealed that a role for tumor associated macrophage in the expansion of gastric cancer stem-like cells and subsequent malignant progression.

Tumor-associated mesothelial cells are negative prognostic factors in gastric cancer and promote peritoneal dissemination of adherent gastric cancer cells by chemotaxis

Tumor Biology ◽  
2014 ◽  
Vol 35 (6) ◽  
pp. 6105-6111 ◽  
Author(s):  
Zhi-Feng Miao ◽  
Ting-Ting Zhao ◽  
Zhen-Ning Wang ◽  
Feng Miao ◽  
Ying-Ying Xu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prognostic Factors ◽  
Cancer Cells ◽  
Peritoneal Dissemination ◽  
Mesothelial Cells ◽  
Gastric Cancer Cells

The mechanism of cleavage of EGFR ligands induced by inflammatory cytokines in gastric cancer cells

2004 ◽  
Vol 127 (2) ◽  
pp. 559-569 ◽  
Author(s):  
Satoshi Tanida ◽  
Takashi Joh ◽  
Keisuke Itoh ◽  
Hiromi Kataoka ◽  
Makoto Sasaki ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Inflammatory Cytokines ◽  
Gastric Cancer Cells ◽  
Egfr Ligands

scholarly journals Induction of Pro-Inflammatory Response via Activated Macrophage-Mediated NF-κB and STAT3 Pathways in Gastric Cancer Cells

2018 ◽  
Vol 47 (4) ◽  
pp. 1399-1410 ◽  
Author(s):  
Yujuan Zhou ◽  
Longzheng Xia ◽  
Qiang Liu ◽  
Heran Wang ◽  
Jingguan Lin ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Proliferation ◽  
Inflammatory Response ◽  
Cancer Cells ◽  
Signalling Pathways ◽  
Activated Macrophages ◽  
Gastric Cancer Cells ◽  
Qrt Pcr ◽  
Stat3 Signalling ◽  
Epithelium Cells

Background/Aims: Chronic inflammation plays an important role in the initiation and progression of gastric cancer (GC). However, the role and relationship of activated macrophages with gastric mucous epithelium cells in initiating and maintaining the inflammatory process during gastric carcinogenesis remains unclear. Methods: The tumour associated macrophages (TAMs) density of gastric cancer was characterized by immunohistochemistry, and the relationship between macrophages and gastric epithelium cells was analysed using an in vitro culture system that imitates the inflammatory microenvironment. The production of pro-inflammatory cytokines was detected by enzyme-linked immunosorbent assay (ELISA) and quantitative real-time PCR (qRT-PCR). MTT assays, Western blotting, qRT-PCR, and luciferase reporter assays were used to detect the effects of cell proliferation, as well as the NF-κB and STAT3 signalling pathways. Results: TAMs infiltrated with a high intensity in GC and were significantly correlated with histology grade (P = 0.012), metastasis (P = 0.001), TNM stage (P = 0.002), and poor prognosis in patients (PFS, P = 0.005; OS, P = 0.028). In addition, IL-6 and IL-8 were elevated in the serum of GC patients and significantly promoted the growth of GC. The exposure of BGC823 gastric cancer cells to a conditioned medium from LPS-treated D-THP-1 cells significantly induced the production of TNF-α, IL-6, IL-1β and IL-8 (P< 0.01). LPS and LPS-treated D-THP-1-conditioned media promoted gastric cancer cell proliferation and triggered the significant activation of NF-κB and STAT3 with a concomitant degradation of IκBα and an increase in JAK2 phosphorylation (P < 0.05). Moreover, gastric cancer cells markedly expressed cell membrane LPS receptors, such as TLR1, TLR4, TLR6, CD14 and MD2. Conclusions: TAMs are closely associated with the growth of GC and prognosis in GC patients. GC cells may directly sustain and amplify the local pro-inflammatory response upon encountering activated macrophages and LPS via NF-κB and STAT3 signalling pathways, thereby promoting tumour progression.

scholarly journals Astragalus extract inhibits destruction of gastric cancer cells to mesothelial cells by anti-apoptosis

2009 ◽  
Vol 15 (5) ◽  
pp. 570 ◽  
Author(s):  
Di Na ◽  
Fu-Nan Liu ◽  
Zhi-Feng Miao ◽  
Zong-Min Du ◽  
Hui-Mian Xu
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Mesothelial Cells ◽  
Gastric Cancer Cells
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