Pathological study and detection of Bovine parainfluenza 3 virus in pneumonic sheep lungs using direct immunofluorescence antibody technique

A subsample consisting of fifty fecal samples from wild Iberian Wolf (Canis lupus signatus), from the northwest of Spain were collected in the field. The samples were analyzed for cysts of Giardia sp. and oocysts of Cryptosporidium sp. using a direct immunofluorescence antibody test (IFA). Giardia sp. and Cryptosporidium sp. were found in 20.0 % of the samples examined. Simple infections were more frequent (90.0 %) with seven (14.0 %) positive for Giardia sp. and two (4.0 %) positive for Cryptosporidium sp. To the authors’ knowledge, this is the first report of occurrence of Cryptosporidium sp. in Iberian Wolf.

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Demonstration of antibodies to Bacillus piliformis in SPF colonies and experimental transplacental infection by Bacillus piliformis in mice

Laboratory Animals ◽

10.1258/002367778780953332 ◽

1978 ◽

Vol 12 (1) ◽

pp. 23-26 ◽

Cited By ~ 14

Author(s):

A. Schaich Fries

Keyword(s):

Antibody Technique ◽

Foetal Membranes ◽

Transplacental Infection ◽

Immunofluorescence Antibody

Antibodies to Bacillus piliformis were demonstrated by the immunofluorescence antibody technique in sera from mice and rabbits from SPF breeding colonies. Mice in various stages of pregnancy were experimentally infected with Bacillus piliformis and killed 2 to 3 days later. The organism was demonstrated in the uterus, foetal membranes and in the liver of the foetuses. Infection was not limited to any particular stage of pregnancy.

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Anti-nuclear antibodies: A practical approach to testing and interpretation

Journal of Skin and Sexually Transmitted Diseases ◽

10.25259/jsstd_40_2020 ◽

2020 ◽

Vol 0 ◽

pp. 1-5

Author(s):

Parvathy Santhosh ◽

Kidangazhiathmana Ajithkumar

Keyword(s):

Screening Tool ◽

Solid Phase ◽

Connective Tissue Diseases ◽

High Sensitivity ◽

Clinical Suspicion ◽

Test Results ◽

Antibody Technique ◽

Common Technique ◽

Asymptomatic Individuals ◽

Immunofluorescence Antibody

Anti-nuclear antibodies (ANAs) are a group of antibodies that are characteristically associated with connective tissue diseases (CTDs). Indirect immunofluorescence antibody technique, having a high sensitivity, is the most common technique used for detection, results of which are expressed in terms of the pattern of fluorescence, substrate used, and the titer of a positive test. Other methods include solid-phase assays. ANA test must be performed only when there is a clinical suspicion of an autoimmune CTD. ANA should not be used as a screening tool for asymptomatic individuals. It is essential in clinical practice to be aware of when to order ANA testing, and how to correctly interpret the test results.

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Occurrence of Giardia Cysts in Mussels (Mytilus galloprovincialis) Destined for Human Consumption

Journal of Food Protection ◽

10.4315/0362-028x-68.8.1702 ◽

2005 ◽

Vol 68 (8) ◽

pp. 1702-1705 ◽

Cited By ~ 13

Author(s):

HIPÓLITO GÓMEZ-COUSO ◽

FERNANDO MÉNDEZ-HERMIDA ◽

JOSÉ ANTONIO CASTRO-HERMIDA ◽

ELVIRA ARES-MAZÁS

Keyword(s):

Mytilus Galloprovincialis ◽

Antibody Test ◽

Most Probable Number ◽

Direct Immunofluorescence ◽

Human Consumption ◽

Microbiological Contamination ◽

Probable Number ◽

Immunofluorescence Antibody Test ◽

Immunofluorescence Antibody ◽

Giardia Cysts

Between January and June 2004, a total of 200 nondepurated mussel samples of the Galician coast (northwest Spain) were examined for Giardia cysts with a direct immunofluorescence antibody test. Giardia cysts were found in mussels from all of the estuaries studied, with an overall rate of contamination of 41.5%. There was relation between the presence of Giardia cysts, the microbiological contamination (expressed as most probable number of Escherichia coli) detected in the samples, and the harvesting area. This is the first work that describes the presence of Giardia cysts in mussels (Mytilus galloprovincialis) destined for human consumption.

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Immunocytochemical localization of β1-4 galactosyltransferase in endometrial carcinoma cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162284 ◽

1990 ◽

Vol 48 (3) ◽

pp. 944-945

Author(s):

Ichiro Yamamoto ◽

Toshiaki Tachibana ◽

Hiroko Maruyama ◽

Noriyuki Komatsu ◽

Hiroyuki Kuramoto ◽

...

Keyword(s):

Endometrial Carcinoma ◽

Cell Lines ◽

Endometrial Adenocarcinoma ◽

Protein A ◽

Rabbit Antiserum ◽

Carcinoma Cells ◽

Affinity Column ◽

Antibody Technique ◽

Galactosyl Transferase ◽

C Terminus

We have paid attention to the alteration of glycosyltransferase in carcinoma cells, because it might be related to the malignancy of the cells. In this connection, localization of β1-4 galactosyl transferase (β1-4 Gal T) in human endometrial carcinoma cells was examined immunocytochemically using two kinds of cell lines, each of which showed different degree of differentiation.An antibody was purified from the rabbit antiserum against the synthetic peptide, IFNRLVFRGMSC (W89) of human β1-4 Gal T coupled with KLH (keyhole limpet hemocyanine) by protein A column and peptide-affinity column chromatography. The anti-W89 serum reacts to the C-terminus of human β 1-4 Gal T and to both membrane-bound and soluble forms of the enzyme. Cell line of well differentiated endometrial adenocarcinoma (I) and that of poorly differentiated endometrial adenocarcinoma (50B) were cultivated respectively in MEM medium containing 15% FCS and 2 mM glutamine for 4 d at 37°C under 5% CO2. The cells were fixed in a mixture of 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M Soerensen’s phosphate buffer (pH 7.4) at 4°C for 30 min, washed with PBS, then freezed and thawed. The indirect method of the peroxidase- labeled antibody technique was used for immunocytochemistry of both LM and TEM on the cell lines. The cells were dehydrated in ethanol and embedded in TAAB 812. Ultrathin sections were observed under a TEM, JEM-100S.

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Immunoferritin Labelling of Murine Leukemia Virus Antigens in thin Sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002221 ◽

1980 ◽

Vol 38 ◽

pp. 508-509

Author(s):

Ray A. Weigand ◽

Gregory C. Varjabedian

Keyword(s):

Murine Leukemia Virus ◽

Leukemia Virus ◽

Intracellular Localization ◽

Uranyl Acetate ◽

Antibody Technique ◽

Thin Sections ◽

Tumor Virus ◽

Labelling Procedure ◽

Unlabelled Antibody ◽

Murine Leukemia

We previously described the intracellular localization of murine mammary tumor virus (MuMTV) p28 protein in thin sections (1). In that study, MuMTV containing cells fixed in 3% paraformaldehyde plus 0.05% glutaraldehyde were labelled after thin sectioning using ferritin-antiferritin in an unlabelled antibody technique. We now describe the labelling of murine leukemia virus (MuLV) particles using the unlabelled antibody technique coupled to ferritin-Fab antiferritin. Cultures of R-MuLV in NIH/3T3 cells were grown to 90% confluence (2), fixed with 2% paraformaldehyde plus 0.5% glutaraldehyde in 0.1 M cacodylate at pH 7.2, postfixed with buffered 17 OsO4, dehydrated with a series of etha-nols, and embedded in Epon. Thin sections were collected on nickel grids, incubated in 107 H2O2, rinsed in HEPES buffered saline, and subjected to the immunoferritin labelling procedure. The procedure included preincubation in 27 egg albumin, a four hour incubation in goat antisera against purified gp69/71 of MuLV (3) (primary antibody), incubation in F(ab’)2 fragments of rabbit antisera to goat IgG (secondary antibody), incubation in apoferritin, incubation in ferritin-Fab ferritin, and a brief fixation with 2% glutaraldehyde. The sections were stained with uranyl acetate and examined in a Siemens IA electron microscope at an accelerating voltage of 60 KV.

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