The prevention effect of poly (l-glutamic acid)/chitosan on spinal epidural fibrosis and peridural adhesion in the post-laminectomy rabbit model

2014 ◽  
Vol 23 (11) ◽  
pp. 2423-2431 ◽  
Author(s):  
Chunbo Li ◽  
Hong Wang ◽  
Haifei Liu ◽  
Jingbo Yin ◽  
Lei Cui ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Rabbit Model ◽  
Epidural Fibrosis ◽  
Spinal Epidural

Mitomycin C in preventing spinal epidural fibrosis in a laminectomy model in rats

2004 ◽  
Vol 100 (1) ◽  
pp. 52-55 ◽  
Author(s):  
Jin-Yul Lee ◽  
Werner Stenzel ◽  
Heinrich Ebel ◽  
Christoph Wedekind ◽  
Ralf-Ingo Ernestus ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Spinal Column ◽  
Failed Back Surgery Syndrome ◽  
Epidural Fibrosis ◽  
En Bloc ◽  
Content Type ◽  
Failed Back Surgery ◽  
Back Surgery ◽  
Spinal Epidural ◽  
Fine Print

Object. Extensive epidural fibrosis after lumbar surgery may be the underlying cause in most cases of failed—back surgery syndrome. Various materials have been used to prevent epidural fibrosis, but only moderate success has been shown. Mitomycin C, an alkylosing antibiotic substance isolated from Streptomyces caespitosus, potentially supresses fibroblast proliferation after surgery. In this study, the authors investigated the effect of mitomycin C by local application on spinal epidural fibrosis in a rat laminectomy model. Methods. Five Wistar rats underwent laminectomy at cervical, thoracic, and lumbar levels. Based on data obtained from ophthalmological studies, mitomycin C was applied to the laminectomy sites in various concentrations (0.01, 0.05, and 0.1 mg/ml). One laminectomy site in each rat was left untreated and thus served as a control. Evoked potentials were measured pre- and postoperatively, and all rats underwent clinical evaluation. Mobility status and evidence of neurological deficit were recorded. Twelve weeks later, the rats were killed, and the spinal column, including surrounding muscle tissue, was removed en bloc, decalcified, and fixed in formaldehyde. Epidural fibrosis was evaluated histologically. In all mitomycin C—treated laminectomy sites, epidural scarring was significantly reduced compared with control sites. Remarkably, dural adhesions were absent in laminectomy defects treated with mitomycin C concentrations of 0.05 and 0.1 mg/ml. Moderate to marked epidural fibrosis with adhesion to the dura mater was noted at sites receiving 0.01 mg/ml of mitomycin C. All control sites showed dense epidural fibrosis with marked dura adherence. Conclusions. In this experimental model, mitomycin C applied locally at a concentration of 0.1 mg/ml effectively reduced epidural fibrosis, completely avoided dural adherence, and induced no side effects.

Spinal epidural fibrosis following hemostatic agent employment

2021 ◽  
pp. 1-5
Author(s):  
Ismail Bozkurt ◽  
Atilla Kazanci ◽  
Oktay Gurcan ◽  
Ahmet G. Gurcay ◽  
Ata T. Arikok ◽  
...  
Keyword(s):  
Hemostatic Agent ◽  
Epidural Fibrosis ◽  
Spinal Epidural

The effect of mitomycin C in reducing epidural fibrosis after lumbar laminectomy in rats

2006 ◽  
Vol 5 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Jin-Yul Lee ◽  
Werner Stenzel ◽  
Peter Impekoven ◽  
Martin Theisohn ◽  
Hartmut Stützer ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Fibrous Tissue ◽  
Lumbar Spine Surgery ◽  
Severe Bleeding ◽  
Epidural Fibrosis ◽  
Mr Images ◽  
Radiological Investigation ◽  
Similar Treatment ◽  
Failed Back Syndrome ◽  
Spinal Epidural

Object xtensive epidural fibrosis after lumbar spine surgery might be an important underlying cause of failed-back syndrome. Based on previously obtained data, the effect of mitomycin C (MMC) in a concentration of 0.1 mg/ml on spinal epidural fibrosis in a rat laminectomy model was investigated in a large series. Methods ighty adult Wistar rats underwent lumbar laminectomy. In 40 rats, MMC in a concentration of 0.1 mg/ml was locally applied to the laminectomy sites. No similar treatment was performed in the other 40 rats. At intervals from one to 12 weeks after laminectomy, both macroscopic and histological evaluations were performed. For radiological investigation, 10 rats underwent magnetic resonance (MR) imaging at 6 weeks postoperatively. Furthermore, the concentration of MMC in cerebrospinal fluid (CSF) and serum was determined 12 hours postoperatively in seven rats. Due to ease of absorption, high levels of MMC were rapidly detectable in serum, whereas the values obtained from the CSF were markedly lower. In the majority of MMC-treated laminectomy sites, epidural scarring was significantly reduced and dural adhesions were absent, in comparison with control sites (p < 0.001), as confirmed by MR images. Accordingly, the macroscopic dissection of epidural fibrous tissue to reexpose the dura mater was performed more easily and without severe bleeding in these rats. The healing of skin and the lumbar fascia was not affected, and dural leakage was not observed. All control sites showed dense epidural fibrosis with marked dural adherence. Conclusions n this experimental model, it was shown that locally applied MMC in a concentration of 0.1 mg/ml effectively reduces epidural fibrosis and dural adherence without side effects in rats that underwent lumbar laminectomy.

Topical use of colchicine to prevent spinal epidural fibrosis in rats

2010 ◽  
Vol 32 (10) ◽  
pp. 1117-1120 ◽  
Author(s):  
Ozgur Ozdemir ◽  
Tarkan Calisaneller ◽  
Erkin Sonmez ◽  
Halil Kiyici ◽  
Hakan Caner ◽  
...  
Keyword(s):  
Epidural Fibrosis ◽  
Spinal Epidural

Use of pimecrolimus to prevent epidural fibrosis in a postlaminectomy rat model

2009 ◽  
Vol 11 (6) ◽  
pp. 758-763 ◽  
Author(s):  
Berker Cemil ◽  
Kagan Tun ◽  
Erkan Kaptanoglu ◽  
Figen Kaymaz ◽  
Banu Cevirgen ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Dura Mater ◽  
Inflammatory Diseases ◽  
Scar Tissue ◽  
Control Group ◽  
Epidural Fibrosis ◽  
Treatment Groups ◽  
Lumbar Level ◽  
Spinal Epidural ◽  
Preventive Effects

Object Epidural fibrosis is the scar tissue formed over the dura mater after a laminectomy. Extensive epidural fibrosis may be an important underlying cause of failed back syndrome. Pimecrolimus, an ascomycin derivative, is one of the new classes of immunomodulating macrolactams and was specifically developed for the treatment of inflammatory diseases. This study examined the preventive effects of the local application of pimecrolimus in minimizing spinal epidural fibrosis in a rat laminectomy model. Methods Thirty Wistar rats were divided into 3 equal groups: control, mitomycin C (MMC), and pimecrolimus groups. Each rat underwent a laminectomy at the L-3 lumbar level. In the experimental groups, a cotton pad soaked with MMC (0.5 mg/ml) or 5 mg pimecrolimus was placed on the exposed dura mater. No treatment was performed in the control group rats. Thirty days after surgery, the rats were killed and the dura mater thickness, epidural fibrosis, and arachnoidal involvement were quantified. Results The mean dura thickness was measured at 9.28 ± 3.39 μm in the MMC group and at 8.69 ± 2.32 μm in the pimecrolimus group, compared with 14.70 ± 4.14 μm in the control group. In addition, the epidural fibrosis and arachnoidal involvement were reduced significantly in the treatment groups compared with the control group. Conclusions In this animal model, it was shown that locally applied pimecrolimus effectively reduces epidural fibrosis and dural adherence in rats that underwent lumbar laminectomy. Mitomycin C was equally effective as pimecrolimus in reducing epidural fibrosis and dural adherence in this study.

scholarly journals Topically Applied Cross-Linked Hyaluronan Attenuates the Formation of Spinal Epidural Fibrosis in a Swine Model of Laminectomy

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Cheng-Li Lin ◽  
I-Ming Jou ◽  
Cheng-Yi Wu ◽  
Yuh-Ruey Kuo ◽  
Shih-Chieh Yang ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Healing Process ◽  
Scar Tissue ◽  
Control Group ◽  
Swine Model ◽  
Epidural Fibrosis ◽  
Mesenchymal Transition ◽  
Failed Back Surgery ◽  
Anti Inflammatory ◽  
Spinal Epidural

Abstract Epidural fibrosis is an inevitable aspect of the postoperative healing process which is one of the causes of failed back surgery syndrome following spinal surgery. The aim of the present study was to examine the inhibitory effect of 1,4-butanediol diglycidyl ether-crosslinked hyaluronan (cHA) on spinal epidural fibrosis in a swine model. Epidural fibrosis was induced through conduction of hemi-laminotomy (L2 and L3) or laminectomy (L4 and L5), while L1 was assigned as the control group in six pigs. The cHA was applied to L3 and L5 surgical sites. MRI evaluation, histologic examination, expressions of matrix metalloproteinases (MMPs), and cytokines in scar tissue were assessed four months after surgery. cHA treatment significantly decreased the scar formation in both hemi-laminotomy and laminectomy sites. cHA also significantly increased MMP-3 and MMP-9 expression in scar tissue. Further, the epithelial-mesenchymal transition -related factors (transforming growth factor-β and vimentin) were suppressed and the anti-inflammatory cytokines (CD44 and interleukin-6) were increasingly expressed in cHA-treated sites. The current study demonstrated that cHA may attenuate spinal epidural fibrosis formation after laminectomy surgery by enhancing the expression of MMPs and anti-inflammatory pathways.

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