A bare-eye-based lateral flow immunoassay based on the use of gold nanoparticles for simultaneous detection of three pesticides

2014 ◽  
Vol 181 (13-14) ◽  
pp. 1565-1572 ◽  
Author(s):  
Limin Wang ◽  
Jia Cai ◽  
Yulong Wang ◽  
Qingkui Fang ◽  
Suyan Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

Gold nanoparticles-based lateral flow immunoassay with silver staining for simultaneous detection of fumonisin B1 and deoxynivalenol

Food Control ◽  
2015 ◽  
Vol 54 ◽  
pp. 347-352 ◽  
Author(s):  
Qing Yu ◽  
Heng Li ◽  
Chenglong Li ◽  
Suxia Zhang ◽  
Jianzhong Shen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Silver Staining ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

scholarly journals The simultaneous detection of the squamous cell carcinoma antigen and cancer antigen 125 in the cervical cancer serum using nano-Ag polydopamine nanospheres in an SERS-based lateral flow immunoassay

RSC Advances ◽  
2020 ◽  
Vol 10 (49) ◽  
pp. 29156-29170
Author(s):  
Ji Xia ◽  
Yifan Liu ◽  
Menglin Ran ◽  
Wenbo Lu ◽  
Liyan Bi ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Squamous Cell Carcinoma Antigen ◽  
Cancer Antigen 125 ◽  
Cancer Antigen

Based on SERS-based lateral flow immunoassay, nano-Ag polydopamine nanospheres was used for detecting squamous cell carcinoma antigen and cancer antigen 125 simultaneously in cervical cancer serum.

Lateral flow immunoassay for 5-hydroxyflunixin based on near-infrared fluorescence molecule as an alternative label to gold nanoparticles

2020 ◽  
Vol 187 (6) ◽  
Author(s):  
Ruiqi Fan ◽  
Wanjun Zhang ◽  
Yongpeng Jin ◽  
Rongmao Zhao ◽  
Chunjiang Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Near Infrared ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Near Infrared Fluorescence

scholarly journals Sensitive and Simultaneous Detection of SARS-CoV-2-Specific IgM/IgG Using Lateral Flow Immunoassay Based on Dual-Mode Quantum Dot Nanobeads

2020 ◽  
Vol 92 (23) ◽  
pp. 15542-15549
Author(s):  
Chongwen Wang ◽  
Xingsheng Yang ◽  
Bing Gu ◽  
Haifeng Liu ◽  
Zihui Zhou ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Simultaneous Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Dual Mode

Lateral Flow Immunoassay Based on Polydopamine-Coated Gold Nanoparticles for the Sensitive Detection of Zearalenone in Maize

2019 ◽  
Vol 11 (34) ◽  
pp. 31283-31290 ◽  
Author(s):  
Shaolan Xu ◽  
Ganggang Zhang ◽  
Bolong Fang ◽  
Qirong Xiong ◽  
Hongwei Duan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Lateral Flow Immunoassay

Development of Silver-Coated Gold Nanoparticles and its Conjugation for Labeling on Lateral Flow Immunoassay

2014 ◽  
Vol 1024 ◽  
pp. 273-276
Author(s):  
Siti Rabizah Makhsin ◽  
Khairunisak Abdul Razak ◽  
Rahmah Noordin
Keyword(s):  
Gold Nanoparticles ◽  
Growth Stage ◽  
Silver Ions ◽  
Biological Properties ◽  
Lateral Flow ◽  
Synthesis Process ◽  
Lateral Flow Immunoassay ◽  
Minimum Concentration ◽  
Colloidal Form ◽  
Washing Process

This work describes the formation of silver-coated gold nanoparticles (Ag-AuNPs) using the seeding-growth method. 15 nm AuNPs seeds were synthesized using the citrate reduction method. In the growth stage, the adding sequence of seeds, ascorbic acid (AA), gold chloride (HAuCl4) and silver nitrate (AgNO3) was explored. The effect AgNO3volume (20 mM) was varied at 0.10, 0.25, 0.50, 1.00, 1.25 and 1.50 ml. Morphology of Ag-AuNPs was observed using a Transmission Electron Microscope (TEM) while UV-Vis Spectrophotometer was used to study the concentration and absorption spectra of colloidal Ag-AuNPs. Zeta-sizer analysis was used to study the particle size distribution of Ag-AuNPs in colloidal form. In the growth stage, the optimum adding sequence was found by adding AA as a reducing agent into AuNPs seeds followed by the addition of HAuCl4and AgNO3. This reshuffle sequence is chosen due to the presence of two absorbance peaks at 398 nm and 501 nm compared to others. From TEM images, increasing concentration of silver ions affected the optical properties, end size of Ag-AuNPs as well as raises the concentration of the colloids suspension. The biological properties of samples Ag-AuNPs (0.1 ml to 0.5 ml AgNO3) were studied by performing the conjugation process between samples and antibody; goat anti-human IgA (GaHIgA). An additional washing process was required to perform conjugation for Ag-AuNPs in order to remove by-product or unreacted chemical produced during synthesis process. The Ag-AuNPs was successfully conjugated with GaHIgA and required a minimum concentration of antibody (9µg/ml) after washing process. In addition, the washed Ag-AuNPs also improved its binding capability with GaHIgA by giving higher intense signal when tested with lined Human IgA on the lateral flow immunoassay (LFI) compared to un-washed Ag-AuNPs.

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