Amperometric immunoassay for the detection of Salmonella pullorum using a screen - printed carbon electrode modified with gold nanoparticle-coated reduced graphene oxide and immunomagnetic beads

2015 ◽  
Vol 183 (2) ◽  
pp. 757-764 ◽  
Author(s):  
Jianfeng Fei ◽  
Wenchao Dou ◽  
Guangying Zhao
Keyword(s):  
Graphene Oxide ◽  
Gold Nanoparticle ◽  
Reduced Graphene Oxide ◽  
Carbon Electrode ◽  
Immunomagnetic Beads ◽  
Screen Printed Carbon Electrode ◽  
Reduced Graphene ◽  
Salmonella Pullorum ◽  
Screen Printed

scholarly journals Tyrosinase/Chitosan/Reduced Graphene Oxide Modified Screen-Printed Carbon Electrode for Sensitive and Interference-Free Detection of Dopamine

2019 ◽  
Vol 9 (4) ◽  
pp. 622 ◽  
Author(s):  
Cheng-You Liu ◽  
Yi-Chieh Chou ◽  
Jui-Hsuan Tsai ◽  
Tzu-Ming Huang ◽  
Jian-Zhang Chen ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Electrochemical Sensors ◽  
High Sensitivity ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Reduced Graphene ◽  
Sensitivity And Selectivity ◽  
Cv Measurements ◽  
Screen Printed

Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection’s specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4–8 μM and 40–500 μM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples.

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