Voltammetric sandwich immunoassay for Cronobacter sakazakii using a screen-printed carbon electrode modified with horseradish peroxidase, reduced graphene oxide, thionine and gold nanoparticles

2017 ◽  
Vol 185 (1) ◽  
Author(s):  
Fanjun Zhu ◽  
Guangying Zhao ◽  
Wenchao Dou
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Horseradish Peroxidase ◽  
Reduced Graphene Oxide ◽  
Sandwich Immunoassay ◽  
Carbon Electrode ◽  
Cronobacter Sakazakii ◽  
Screen Printed Carbon Electrode ◽  
Reduced Graphene ◽  
Screen Printed

scholarly journals Tyrosinase/Chitosan/Reduced Graphene Oxide Modified Screen-Printed Carbon Electrode for Sensitive and Interference-Free Detection of Dopamine

2019 ◽  
Vol 9 (4) ◽  
pp. 622 ◽  
Author(s):  
Cheng-You Liu ◽  
Yi-Chieh Chou ◽  
Jui-Hsuan Tsai ◽  
Tzu-Ming Huang ◽  
Jian-Zhang Chen ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Electrochemical Sensors ◽  
High Sensitivity ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Reduced Graphene ◽  
Sensitivity And Selectivity ◽  
Cv Measurements ◽  
Screen Printed

Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection’s specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4–8 μM and 40–500 μM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples.

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