Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe

Abstract The enzymatic determination of serum uric acid by use of uricase, catalase, and aldehyde dehydrogenase according to Haeckel [J. Clin. Chem. Clin Biochem. 14, 101 (1976)] showed interferences from ethanol-converting enzymes, which are present in some patients' sera. We have identified these enzymes as alcohol dehydrogenase isoenzymes. Among other substances, a mixture of pyrazole and oxalate can be used to eliminate these interferences. This inhibitor system gives good results when used in the automated kinetic uric acid determination, as is shown by a comparison with the manual assay for uric acid according to Kageyama [Clin. Chim. Acta 31, 421 (1971)].

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A dual-signal colorimetric and ratiometric fluorescent nanoprobe for enzymatic determination of uric acid by using silicon nanoparticles

Microchimica Acta ◽

10.1007/s00604-019-3862-2 ◽

2019 ◽

Vol 186 (12) ◽

Cited By ~ 4

Author(s):

Cuiyan Wu ◽

Lijun Zhu ◽

Qiujun Lu ◽

Haitao Li ◽

Youyu Zhang ◽

...

Keyword(s):

Uric Acid ◽

Silicon Nanoparticles ◽

Fluorescent Nanoprobe ◽

Enzymatic Determination

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Sonication-assisted preparation of a nanocomposite consisting of reduced graphene oxide and CdSe quantum dots, and its application to simultaneous voltammetric determination of ascorbic acid, dopamine and uric acid

Microchimica Acta ◽

10.1007/s00604-018-2988-y ◽

2018 ◽

Vol 185 (10) ◽

Cited By ~ 9

Author(s):

Ebrahim Tavakolian ◽

Javad Tashkhourian

Keyword(s):

Quantum Dots ◽

Ascorbic Acid ◽

Uric Acid ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Voltammetric Determination ◽

Cdse Quantum Dots ◽

Reduced Graphene ◽

Simultaneous Voltammetric Determination

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The Automated Determination of Serum Uric Acid

Clinical Chemistry ◽

10.1093/clinchem/12.11.748 ◽

1966 ◽

Vol 12 (11) ◽

pp. 748-766 ◽

Cited By ~ 29

Author(s):

Stanley Morgenstern ◽

Richard V Flor ◽

James H Kaufman ◽

Bernard Klein

Keyword(s):

Uric Acid ◽

Serum Uric Acid ◽

Enzymatic Reaction ◽

Enzymatic Determination ◽

Before And After ◽

The Difference ◽

Automated Determination ◽

Colorimetric Reaction ◽

Good Agreement

Abstract An automated procedure is presented for the enzymatic determination of serum uric acid on both the AutoAnalyzer and the Robot Chemist. The procedure measures as the neocuproine complex, the difference in the amount of Cu+ formed by reaction of a Cu++-alkanolamine buffered solution with serum uric acid under precisely controlled conditions before and after uricase treatment of the serum. The difference is proportional to the true serum uric acid content. The elements contributing to the enzymatic reaction, the colorimetric reaction, and the elimination of interferences were investigated. Comparison of serum uric acid values obtained by this method with those obtained by ultraviolet spectrophotometry show very good agreement.

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β-Cyclodextrin functionalized carbon quantum dots as sensors for determination of water-soluble C60 fullerenes in water

The Analyst ◽

10.1039/c5an01910a ◽

2016 ◽

Vol 141 (9) ◽

pp. 2682-2687 ◽

Cited By ~ 13

Author(s):

Angelina Cayuela ◽

M. Laura Soriano ◽

Miguel Valcárcel

Keyword(s):

Quantum Dots ◽

Direct Determination ◽

Carbon Quantum Dots ◽

Water Soluble ◽

Soluble C ◽

Water Soluble C ◽

C60 Fullerenes

A selective photoluminescence method based on Carbon Quantum Dots (CQDs) functionalized with carboxymethyl-β-cyclodextrin for the direct determination of water-soluble C60 fullerene has been developed.

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