A dual-signal colorimetric and ratiometric fluorescent nanoprobe for enzymatic determination of uric acid by using silicon nanoparticles

2019 ◽  
Vol 186 (12) ◽  
Author(s):  
Cuiyan Wu ◽  
Lijun Zhu ◽  
Qiujun Lu ◽  
Haitao Li ◽  
Youyu Zhang ◽  
...  
Keyword(s):  
Uric Acid ◽  
Silicon Nanoparticles ◽  
Fluorescent Nanoprobe ◽  
Enzymatic Determination

Improved automated kinetic determination of uric acid in serum by use of uricase/catalase/aldehyde dehydrogenase.

1979 ◽  
Vol 25 (4) ◽  
pp. 619-621 ◽  
Author(s):  
K Bartl ◽  
M Brandhuber ◽  
J Ziegenhorn
Keyword(s):  
Uric Acid ◽  
Aldehyde Dehydrogenase ◽  
Alcohol Dehydrogenase Isoenzymes ◽  
Kinetic Determination ◽  
Enzymatic Determination ◽  
Other Substances ◽  
Inhibitor System ◽  
Uric Acid Determination ◽  
Acid Determination

Abstract The enzymatic determination of serum uric acid by use of uricase, catalase, and aldehyde dehydrogenase according to Haeckel [J. Clin. Chem. Clin Biochem. 14, 101 (1976)] showed interferences from ethanol-converting enzymes, which are present in some patients' sera. We have identified these enzymes as alcohol dehydrogenase isoenzymes. Among other substances, a mixture of pyrazole and oxalate can be used to eliminate these interferences. This inhibitor system gives good results when used in the automated kinetic uric acid determination, as is shown by a comparison with the manual assay for uric acid according to Kageyama [Clin. Chim. Acta 31, 421 (1971)].

The Automated Determination of Serum Uric Acid

1966 ◽  
Vol 12 (11) ◽  
pp. 748-766 ◽  
Author(s):  
Stanley Morgenstern ◽  
Richard V Flor ◽  
James H Kaufman ◽  
Bernard Klein
Keyword(s):  
Uric Acid ◽  
Serum Uric Acid ◽  
Enzymatic Reaction ◽  
Enzymatic Determination ◽  
Before And After ◽  
The Difference ◽  
Automated Determination ◽  
Colorimetric Reaction ◽  
Good Agreement

Abstract An automated procedure is presented for the enzymatic determination of serum uric acid on both the AutoAnalyzer and the Robot Chemist. The procedure measures as the neocuproine complex, the difference in the amount of Cu+ formed by reaction of a Cu++-alkanolamine buffered solution with serum uric acid under precisely controlled conditions before and after uricase treatment of the serum. The difference is proportional to the true serum uric acid content. The elements contributing to the enzymatic reaction, the colorimetric reaction, and the elimination of interferences were investigated. Comparison of serum uric acid values obtained by this method with those obtained by ultraviolet spectrophotometry show very good agreement.

Direct ultraviolet method for enzymatic determination of uric acid, with equilibrium and kinetic data-processing options.

1986 ◽  
Vol 32 (3) ◽  
pp. 486-491 ◽  
Author(s):  
B A Dilena ◽  
M J Peake ◽  
H L Pardue ◽  
J W Skoug
Keyword(s):  
Uric Acid ◽  
Data Processing ◽  
Kinetic Data ◽  
Reference Method ◽  
Plasma Equilibrium ◽  
Enzymatic Determination ◽  
Nonlinear Curve Fitting ◽  
High Concentrations ◽  
Nonlinear Curve

Abstract We developed and evaluated a direct ultraviolet method for the enzymatic determination of uric acid in serum, plasma, or urine, without deproteinization of sera and plasma. Equilibrium and nonlinear curve-fitting kinetic options are evaluated and compared, and results of the proposed method are compared with those of a candidate Reference Method. All data-processing options yield a linear relation for absorbance and concentration of uric acid between 0.1 and 2 mmol/L; with the equilibrium option, results are linear to 5 mmol/L. For 100 plasma samples, results correlate well between the proposed method (y) and a reference method (x): y = 0.99x - 0.002 mmol/L. Between-run imprecision is about 2.3%, and interference by hemolyzed, icteric, or lipemic specimens or specimens containing high concentrations of xanthine or paraproteins is minimal. The kinetic option with a data-processing range of 100 s or longer yields results that correlate well with the equilibrium method: y = 1.006x + 0.002 mmol/L (n = 118). For 20 urine samples processed by the proposed (y) and a reference (x) methods, y = 1.04x + 0.038 mmol/L.

Enzymatic determination of uric acid using water-soluble CuInS/ZnS quantum dots as a fluorescent probe

2018 ◽  
Vol 185 (11) ◽  
Author(s):  
Fangmei Zhang ◽  
Pinyi Ma ◽  
Xinyu Deng ◽  
Ying Sun ◽  
Xinghua Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Uric Acid ◽  
Fluorescent Probe ◽  
Water Soluble ◽  
Enzymatic Determination

Enzymatic Determination of Uric Acid with Detailed Directions

1953 ◽  
Vol 5 (3) ◽  
pp. 273-280 ◽  
Author(s):  
E. Prætorius ◽  
H. Poulsen
Keyword(s):  
Uric Acid ◽  
Enzymatic Determination

Non-enzymatic Determination of Uric Acid by Ion Exchange Voltammetry at a Permselective Electrochemical Sensing Platform

2015 ◽  
Vol 5 (3) ◽  
pp. 127-134 ◽  
Author(s):  
Pankaj Kumar Rastogi ◽  
Rupali Gupta ◽  
Shiv Prakash ◽  
Vellaichamy Ganesan
Keyword(s):  
Uric Acid ◽  
Ion Exchange ◽  
Electrochemical Sensing ◽  
Sensing Platform ◽  
Enzymatic Determination

Measurement of Uric Acid in Biologic Fluids Using an Ion-Exchange Separation

1957 ◽  
Vol 3 (3) ◽  
pp. 169-177 ◽  
Author(s):  
Bernard Shapiro ◽  
David Seligson ◽  
Ralph Jessar
Keyword(s):  
Uric Acid ◽  
Ion Exchange ◽  
Quantitative Determination ◽  
Clinical Laboratory ◽  
Direct Methods ◽  
Urine Samples ◽  
Enzymatic Determination ◽  
Ion Exchange Separation ◽  
Routine Clinical Laboratory

Abstract A new method for the measurement of uric acid in biologic fluids is presented. It involves the isolation of uric acid by ion-exchange separation and quantitative determination of uric acid in the eluate by ultraviolet absorption, the Folin-Brown method or an enzymatic technic. The enzymatic determination on the eluate is the most specific of the technics. A series of determinations of uric acid in serum compares well with determinations on the same samples using the direct Folin-Brown procedure. The direct Folin-Brown method appears to be in error in determinations on certain urine samples. Measurements of uric acid in bile are reported. The technic can be used in the routine clinical laboratory, and is particularly applicable to fluids which cannot be studied by direct methods.

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