scholarly journals Interfamilial relationships in order Fabales: new insights from the nuclear regions sqd1 and 26S rDNA

2020 ◽  
Vol 306 (4) ◽  
Author(s):  
Deniz Aygoren Uluer ◽  
Julie A. Hawkins ◽  
Félix Forest
Keyword(s):  
26S Rdna ◽  
Botany ◽  
2011 ◽  
Vol 89 (10) ◽  
pp. 715-730 ◽  
Author(s):  
Mihai Costea ◽  
Ignacio García Ruiz ◽  
Saša Stefanović

The Cuscuta chapalana complex (Convolvulaceae) is a recently circumscribed clade within the subgenus Grammica that includes several little-known species from Mexico and Central and northern South America characterized by the presence of peculiar subapical multicelullar appendages on the corolla and often on the calyx lobes. Basic morphology, scanning electron microscopy, and DNA sequence data from the plastid trnL–F region and the 26S rDNA and internal transcribed spacer nuclear regions were used to investigate the phylogenetic relationships within the group and to test the species limits. Multicellular appendages are horn-like or tubular in shape and bear one or more stomata at their tips; hence the vernacular name “horned” dodders. Eight lineages were reconstructed; two of them, both from Mexico, were described as new species: Cuscuta bonafortunae Costea & I. García, sp. nov., and Cuscuta carnosa Costea & Stefanović, sp. nov. Cuscuta pringlei is redefined as a variety of Cuscuta strobilacea . A taxonomic treatment with an identification key, descriptions, and illustrations is provided.


Author(s):  
D.L. Spector ◽  
S. Huang ◽  
S. Kaurin

We have been interested in the organization of RNA polymerase II transcription and pre-mRNA splicing within the cell nucleus. Several models have been proposed for the functional organization of RNA within the eukaryotic nucleus and for the relationship of this organization to the distribution of pre-mRNA splicing factors. One model suggests that RNAs which must be spliced are capable of recruiting splicing factors to the sites of transcription from storage and/or reassembly sites. When one examines the organization of splicing factors in the nucleus in comparison to the sites of chromatin it is clear that splicing factors are not localized in coincidence with heterochromatin (Fig. 1). Instead, they are distributed in a speckled pattern which is composed of both perichromatin fibrils and interchromatin granule clusters. The perichromatin fibrils are distributed on the periphery of heterochromatin and on the periphery of interchromatin granule clusters as well as being diffusely distributed throughout the nucleoplasm. These nuclear regions have been previously shown to represent initial sites of incorporation of 3H-uridine.


Author(s):  
David L. Spector ◽  
Robert J. Derby

Studies in our laboratory are involved in evaluating the structural and functional organization of the mammalian cell nucleus. Since several major classes (U1, U2, U4/U6, U5) of small nuclear ribonucleoprotein particles (snRNPs) play a crucial role in the processing of pre-mRNA molecules, we have been interested in the localization of these particles within the cell nucleus. Using pre-embedding immunoperoxidase labeling combined with 3-dimensional reconstruction, we have recently shown that nuclear regions enriched in snRNPs form a reticular network within the nucleoplasm which extends between the nucleolar surface and the nuclear envelope. In the present study we were inte rested in extending these nuclear localizations using cell preparation techniques which avoid slow penetration of fixatives, chemical crosslinking of potential antigens and solvent extraction. CHOC 400 cells were cryofixed using a CF 100 ultra rapid cooling device (LifeCell Corp.). After cryofixation cells were molecular distillation dried, vapor osmicated, in filtra ted in 100% Spurr resin in vacuo and polymerized in molds a t 60°C. Using this procedure we were able to evaluate the distribution of snRNPs in resin embedded cells which had not been chemically fixed, incubated in cryoprotectants or extracted with solvents.


Planta Medica ◽  
2009 ◽  
Vol 76 (04) ◽  
pp. 399-401 ◽  
Author(s):  
Hongtao Wang ◽  
Hua Sun ◽  
Woo-Saeng Kwon ◽  
Haizhu Jin ◽  
Deok-Chun Yang
Keyword(s):  
26S Rdna ◽  

2006 ◽  
Vol 2 (S235) ◽  
pp. 69-69
Author(s):  
M. R. Seo ◽  
H. B. Ann

AbstractMost dwarf elliptical galaxies except for ‘dE, N’ galaxies which are characterized by young stellar populations in the nuclear regions are located in the high density environment. The colors and spectra of ‘dE, N’ galaxies are different from other types, in the sense that they have redder colors with little emission lines. The majority of dwarf elliptical galaxies are younger than 7 Gyr with metallicity larger than [z/H]=−0.5.


2009 ◽  
Vol 5 (S267) ◽  
pp. 337-337 ◽  
Author(s):  
Linda E. Strubbe ◽  
Eliot Quataert

A star that wanders too close to a massive black hole (BH) is shredded by the BH's tidal gravity. Stellar gas falls back to the BH, releasing a flare of energy. In anticipation of upcoming transient surveys, we predict the light curves and spectra of tidal flares as a function of time, highlighting the unique signatures of tidal flares in the optical and near-IR. Some of the gas initially bound to the BH is likely blown away when the fallback rate is super-Eddington at early times. This outflow produces an optical luminosity comparable to that of a supernova (Figure 1, left panel); such events have durations of ~ 10 days and may have been missed in supernova searches that exclude the nuclear regions of galaxies. When the fallback rate subsides below Eddington, the gas accretes onto the BH via a thin disk whose emission peaks in the UV to soft X-rays. Some of this emission is reprocessed by the unbound stellar debris, producing a spectrum of very broad emission lines, with no corresponding narrow forbidden lines (center panel). These lines are strongest for BHs with MBH ~ 105–106M⊙ and thus optical surveys are particularly sensitive to the lowest mass BHs in galactic nuclei. Calibrating our models to ROSAT and GALEX observations, we predict detection rates for Pan-STARRS, Palomar Transit Factory, and LSST (right panel) and highlight observational challenges in the optical. Pan-STARRS should detect at least several events per year — many more if current theoretical models of super-Eddington outflows are correct. These surveys will significantly improve our knowledge of stellar dynamics in galactic nuclei, the physics of super-Eddington accretion, the demography of intermediate mass BHs, and the role of tidal disruption in the growth of massive BHs.


2001 ◽  
Vol 560 (1) ◽  
pp. 160-167 ◽  
Author(s):  
Santiago Arribas ◽  
Luis Colina ◽  
David Clements

2021 ◽  
Vol 41 ◽  
Author(s):  
Thais G.M. Abreu ◽  
Marjury C. Maronezi ◽  
Ricardo A.R. Uscategui ◽  
Fabiana L. Rocha ◽  
Ivan R.M. Pádua ◽  
...  

ABSTRACT: Objective was to evaluate the accuracy of elastography in the differentiation between normal and cataract lenses One hundred forty-five eyes of 98 dogs were divided into groups according to cataract stage. Forty-twoeyes were submitted to phacoemulsification. Biometric parameters, echogenicity and echotexture patterns of the anterior, posterior and vitreous chambers, lens and retina-choroid-sclera complexes were evaluated by ocular ultrasound in modes A and B. Deformability, and color (blue color = indicated less rigid structures, color red = more rigid structures) of the lenses were evaluated by the elastogram. The shear wave velocity (SWV; m/s) was calculated in three regions of the lens, both in the cortex and in the nucleus. The SWV of nucleus was statistically different between the normal lenses and with cataracts, and between the stages of cataract (P<0.001). Healthy lenses and incipient cataracts had a more rigid nucleus. Mature cataracts presented lowest nuclear rigidity (P<0.001). On cortical region the SWV was significantly higher (P<0.01) in intumescent and incipient cataracts. SWV less than 2.67m/s indicates cataract with a sensitivity of 72% and specificity of 94%. Values lower than 2.23m/s suggest mature cataract, with sensitivity of 71% and specificity of 76%. SWV greater than 2.66 m/s are associated with normal lenses or incipient cataract, presenting sensitivity of 94% and specificity of 84%. Qualitative method allowed differentiation between healthy and affected lenses and the classification of evolutionary stages. There was a correlation between the degree of stiffness of lens in cortical and nuclear regions (p=00165, r=0.37) and between the balanced saline solution quantitative and surgical time (P<0.01, r=0.73). Degree of stiffness of lens did not correlate with parameters of phacoemulsification. Elastographic proved feasible for evaluating the lens of dogs, characterizing the types of cataracts, and demonstrating increased stiffness of the diseased lenses.


Genome ◽  
1996 ◽  
Vol 39 (3) ◽  
pp. 535-542 ◽  
Author(s):  
Concha Linares ◽  
Juan González ◽  
Esther Ferrer ◽  
Araceli Fominaya

A physical map of the locations of the 5S rDNA genes and their relative positions with respect to 18S–5.8S–26S rDNA genes and a C genome specific repetitive DNA sequence was produced for the chromosomes of diploid, tetraploid, and hexaploid oat species using in situ hybridization. The A genome diploid species showed two pairs of rDNA loci and two pairs of 5S loci located on both arms of one pair of satellited chromosomes. The C genome diploid species showed two major pairs and one minor pair of rDNA loci. One pair of subtelocentric chromosomes carried rDNA and 5S loci physically separated on the long arm. The tetraploid species (AACC genomes) arising from these diploid ancestors showed two pairs of rDNA loci and three pairs of 5S loci. Two pairs of rDNA loci and 2 pairs of 5S loci were arranged as in the A genome diploid species. The third pair of 5S loci was located on one pair of A–C translocated chromosomes using simultaneous in situ hybridization with 5S rDNA genes and a C genome specific repetitive DNA sequence. The hexaploid species (AACCDD genomes) showed three pairs of rDNA loci and six pairs of 5S loci. One pair of 5S loci was located on each of two pairs of C–A/D translocated chromosomes. Comparative studies of the physical arrangement of rDNA and 5S loci in polyploid oats and the putative A and C genome progenitor species suggests that A genome diploid species could be the donor of both A and D genomes of polyploid oats. Key words : oats, 5S rDNA genes, 18S–5.8S–26S rDNA genes, C genome specific repetitive DNA sequence, in situ hybridization, genome evolution.


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