Changing characteristics of wet/dry spells during 1961–2008 in Sichuan province, southwest China

2015 ◽  
Vol 127 (1-2) ◽  
pp. 129-141 ◽  
Author(s):  
Jin Huang ◽  
Xin Chen ◽  
Yan Xue ◽  
Jie Lin ◽  
Jinchi Zhang
Keyword(s):  
Southwest China ◽  
Sichuan Province ◽  
Dry Spells

Seven new species of the Stenus cirrus group (Coleoptera, Staphylinidae) from Sichuan, Southwest China

Zootaxa ◽  
2018 ◽  
Vol 4425 (3) ◽  
pp. 527
Author(s):  
LIANG TANG ◽  
SHENG-NAN LIU ◽  
XIN-YU DONG
Keyword(s):  
New Species ◽  
Southwest China ◽  
Sichuan Province ◽  
Key To Species ◽  
Diagnostic Characters

Seven new species of the Stenus cirrus group collected from Sichuan, Southwest China are described: S. cariniventris Tang, Liu & Dong, sp. n., S. jiangrixini Tang, Liu & Dong, sp. n., S. lineatus Tang, Liu & Dong, sp. n., S. emeishanus Tang, Liu & Dong, sp. n., S. jiudingshanus Tang, Liu & Dong, sp. n., S. xichangensis Tang, Liu & Dong, sp. n., S. brevilineatus Tang, Liu & Dong, sp. n. The diagnostic characters of the new species are illustrated, and a key to species of the group from Sichuan Province is provided. 

scholarly journals A new species ofScutellaria(Scutellarioideae, Lamiaceae) from Sichuan Province in southwest China

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3624 ◽  
Author(s):  
Fei Zhao ◽  
En-De Liu ◽  
Hua Peng ◽  
Chun-Lei Xiang
Keyword(s):  
New Species ◽  
Southwest China ◽  
Morphological Characters ◽  
Sichuan Province ◽  
Pollen Grain ◽  
Yellow Corolla ◽  
A New Species

Scutellaria wuana, a new species discovered from a xeric valley in Muli County of Sichuan Province in southwest China, is described and illustrated. Morphologically, the new species shares similarities withS. mairei, but can be readily distinguished by a suite of morphological characters including a white-pubescent erect stem, conspicuous leaf petioles, and a yellow corolla with a trapeziform lower-middle lip lobe. The habitat and distribution ofS. wuanaare also distinctive. The position of the new species withinScutellariais examined in a phylogenetic context using the nuclear ribosomal internal and external transcribed spacers. Additionally, we examine leaf epidermal and pollen grain micromorphology of the new species and putative relatives.

scholarly journals First Report of Chilli veinal mottle virus Infecting Tomato (Solanum lycopersicum) in China

Plant Disease ◽  
2014 ◽  
Vol 98 (11) ◽  
pp. 1589-1589 ◽  
Author(s):  
F.-F. Zhao ◽  
D.-H. Xi ◽  
J. Liu ◽  
X.-G. Deng ◽  
H.-H. Lin
Keyword(s):  
Mosaic Virus ◽  
Southwest China ◽  
Sichuan Province ◽  
Mottle Virus ◽  
Rt Pcr ◽  
Tomato Plants ◽  
First Report ◽  
Two Samples ◽  
Leaf Distortion ◽  
Chilli Veinal Mottle Virus

Chilli veinal mottle virus (ChiVMV), a potyvirus, is widespread over the world. In China, it was first reported in chili pepper (Capsicum annuum) in Hainan Province (south China) in 2006 (2). Subsequently, it was reported in tobacco (Nicotiana tabacum) in Yunnan Province (southwest China) in 2011 (1). Sichuan Province is one of the largest vegetable producing areas of China. In May 2012, tomatoes with leaves displaying virus-infected symptoms like mottling, mosaic, narrowing, or curling were observed in several fields of Chengdu, eastern Sichuan Province, southwest China. Of the 20 fields we investigated, four fields with 90% tomato plants were infected. During 2012 and 2013, six samples were collected from symptomatic tomato leaves based on different symptoms and locations. All six samples were assayed by western blotting using polyclonal antisera (Cucumber mosaic virus [CMV], Tobacco mosaic virus [TMV]) obtained from Agdia (Elkhart) and one antiserum to ChiVMV obtained from Yunnan Academy of Agricultural Science (China). Two samples from Pengzhou and one sample from Shuangliu exhibiting mosaic leaves were positive for TMV, one sample from Pixian exhibiting narrowing leaves was positive for CMV, and the other two samples from Shuangliu exhibiting mottle and leaf distortion were positive for ChiVMV. Total RNAs was extracted from all six samples and healthy tomato leaves using Trizol reagent (Invitrogen), First-strand cDNA synthesis primed with oligo(dT) by SuperScript III Reverse Transcriptase (Invitrogen). RT-PCR was performed using primer pairs ChiVMV-CP F (5′-GCAGGAGAGAGTGTTGATGCTG-3′) and ChiVMV–CP R (5′-(T)16AACGCCAACTATTG-3′), which were designed to direct the amplification of the entire capsid protein (CP) gene and 3′ untranslated region (3′-UTR) of ChiVMV (GenBank Accession No. KC711055). The expected 1,166-bp DNA fragment was amplified from the two tomato samples from Shuangliu that were positive for ChiVMV in the western blot tests, but not from the others. The obtained fragments were purified and cloned into the PMD18-T vector (TaKaRa) and sequenced. The sequencing results showed that the two ChiVMV isolates from tomato in Shuangliu were identical (KF738253). Nucleotide BLAST analysis revealed that this ChiVMV isolate shared ~84 to 99% nucleotide identities with other ChiVMV isolates available in GenBank (KC711055 to KF220408). To fulfill Koch's postulates, we isolated this virus by three cycle single lesion isolation in N. tabacum, and mechanically inoculated it onto tomato leaves. The same mottle and leaf distortion symptoms in systemic leaves were observed. Subsequent RT-PCR, fragment clone, and sequence determination tests were repeated and the results were the same. All the evidence from these tests revealed that the two tomato plants were infected by ChiVMV. To our knowledge, this is the first report of ChiVMV naturally infecting tomato in China. It shows that ChiVMV is spreading in China and is naturally infecting a new solanaceous crop in the southwest area, and the spread of the virus may affect tomato crop yields in China. Thus, it is very important to seek an effective way to control this virus. References: (1) M. Ding et al. Plant Dis. 95:357, 2011. (2) J. Wang et al. Plant Dis. 90:377, 2006.

scholarly journals Molecular Characterization Based on Whole-Genome Sequencing of Streptococcus pneumoniae in Children Living in Southwest China During 2017-2019

2021 ◽  
Vol 11 ◽  
Author(s):  
Ziyi Yan ◽  
Yali Cui ◽  
Xiaocui Huang ◽  
Shikun Lei ◽  
Wei Zhou ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Southwest China ◽  
Pneumococcal Infection ◽  
Sichuan Province ◽  
Coverage Rate ◽  
University Hospital ◽  
Guizhou Province ◽  
Effective Vaccine ◽  
Molecular Characteristics ◽  
High Morbidity

BackgroundStreptococcus pneumoniae is an important pathogen causing high morbidity and high mortality in children and undergoes frequent recombination for capsule switching to neutralize the 13-valent pneumococcal conjugate vaccine (PCV13). This study aimed to investigate the prevalence, and molecular characteristics including serotypes and antibiotic susceptibility of S. pneumoniae isolated from children living in Southwest China from 2017 to 2019 to facilitate the selection of effective vaccine formulations and appropriate antibiotic treatment regimens.MethodsThis study was conducted at West China Second University Hospital (Chengdu, Sichuan Province, China), Zunyi Medical University Third Affiliated Hospital/First People’s Hospital of Zunyi (Zunyi, Guizhou Province, China) and Chengdu Jinjiang District Maternal and Child Healthcare Hospital (Chengdu, Sichuan Province, China). Demographic and clinical characteristics of children infected with S. pneumoniae were collected and analysed. Next-generation sequencing and sequence analysis were used to determine the serotypes, sequence types, antibiotic resistance and potential protein vaccine target genes of the pneumococcal isolates. The coverage rate provided by PCV13 was estimated by calculating the percentage of the specific serotypes that were specifically the PCV13-included serotypes. Antimicrobial susceptibility was determined by the microdilution broth method.ResultsThe most prevalent pneumococcal serotypes were 19F (25.8%), 19A (14.1%), 6B (12.5%), 6A (9.4%) and 14 (7.8%). The predominant STs were ST271 (23.3%), ST320 (15.5%) and ST90 (8.6%), dominated by the clonal complex Taiwan19F-14 (39.1%). The coverage rate of PCV13 was 77.3% in all the isolates, with relatively higher values in invasive isolates (86.4%). Over the decade, the rates of resistance to penicillin, amoxicillin and cefotaxime were 5.6%, 5.3% and 5.1%, respectively, with significantly higher values in invasive isolates (22.4%, 14.9% and 11.9%). Almost all the isolates were resistant to erythromycin (99.1%) and clindamycin (95.9%). All isolates carried virulence-related genes, including ply, psaA, piaA, piuA, phtE, nanA, pepO, danJ, pvaA, clpP, pcsB, stkP, potD, and strH. The carriage of virulence and resistance genes varied among serotypes and clades, with serotype 19F/ST271 showing higher resistance to antibiotics and being more likely to carry pilus genes and other virulence genes.ConclusionThese data provide valuable information for the understanding of pneumococcal pathogenesis, antimicrobial resistance and the development of protein-based vaccines against pneumococcal infection.

Five new Achnanthidiaceae species (Bacillariophyta) from Jiuzhai Valley, Sichuan Province, Southwestern China

Phytotaxa ◽  
2019 ◽  
Vol 405 (3) ◽  
pp. 147
Author(s):  
P. YU ◽  
Q-M. YOU ◽  
W-T. PANG ◽  
Y. CAO ◽  
Q-X. WANG
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Light Microscopy ◽  
Southwest China ◽  
Sichuan Province ◽  
Similar Species ◽  
Separate Species ◽  
Outline Shape ◽  
Scanning Electron

Five new species are described in this study: Achnanthidium jiuzhaienis sp. nov., Achnanthidium epilithica sp. nov., Achnanthidium limosua sp. nov., Achnanthidium subtilissimum sp. nov., and Kolbesia sichuanenis sp. nov. Classification was based on light microscopy (LM) and scanning electron microscopy (SEM). A. jiuzhaienis sp. nov. belongs to the “A. pyrenaicum complex” of the genus Achnanthidium, this classification was based on certain characteristics, such as the possession of transpically-elongated areolae and deflected external distal raphe fissures. A. epilithica sp. nov., A. limosua sp. nov., and A. subtilissimum sp. nov. belong to the “A. minutissimum complex” of the genus Achnanthidium, this classification was based on certain characteristics, such as the possession of straight external distal raphe fissures and round or elliptical areolae. Kolbesia sichuanenis sp. nov. was found to belongs to the Kolbesia genus, based on its elongated areolae and striae comprised of one macroareola. All five species were sufficiently different from other similar species to be considered unique separate species based on their valve outline, shape of the axial and center areas, and density of striae of each taxon. These new species derived from the epilithic and epiphytic collections of four alkaline lakes in Jiuzhai Valley, Sichuan Province, Southwest China.

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