Molecular characterization of domestic and exotic potato virus S isolates and a global analysis of genomic sequences

2014 ◽  
Vol 159 (8) ◽  
pp. 2115-2122 ◽  
Author(s):  
Y.-H. Lin ◽  
J. A. Abad ◽  
C. J. Maroon-Lango ◽  
K. L. Perry ◽  
H. R. Pappu
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Global Analysis ◽  
Genomic Sequences ◽  
Potato Virus S

Molecular characterization of two potato virus S isolates from late-blight-resistant genotypes of potato (Solanum tuberosum)

2009 ◽  
Vol 154 (11) ◽  
pp. 1861-1863 ◽  
Author(s):  
Y. H. Lin ◽  
K. L. Druffel ◽  
J. Whitworth ◽  
M. J. Pavek ◽  
Hanu R. Pappu
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus S ◽  
Resistant Genotypes

Distribution and molecular characterization of potato virus Y (PVY) strains infecting potato (Solanum tuberosum) crop in Kashmir (India)

VirusDisease ◽  
2021 ◽  
Author(s):  
Sumiah Wani ◽  
Sahar Saleem ◽  
Sajad U. Nabi ◽  
Gowhar Ali ◽  
Bilal A. Paddar ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

scholarly journals Detection and molecular characterization of Slovak tomato isolates belonging to two recombinant strains of potato virus Y

2016 ◽  
Vol 60 (04) ◽  
pp. 347-353 ◽  
Author(s):  
N. SIHELSKÁ ◽  
L. PREDAJŇA ◽  
A. NAGYOVÁ ◽  
K. ŠOLTYS ◽  
J. BUDIŠ ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Recombinant Strains

Molecular characterization of potato virus Y resistance in potato (Solanum tuberosumL.)

2015 ◽  
Vol 75 (3) ◽  
pp. 389 ◽  
Author(s):  
Vinay Bhardwaj ◽  
Reena Sharma ◽  
Dalamu ◽  
A. K. Srivastava ◽  
R. Baswaraj ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus Y

Molecular Characterization of Potato leafroll virus, Potato virus A, and Potato virus X Isolates from Potatoes in Alaskan Cities and Villages

2011 ◽  
Vol 12 (1) ◽  
pp. 39 ◽  
Author(s):  
Nancy L. Robertson ◽  
Jeffrey Smeenk ◽  
Jodie M. Anderson
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Potato Virus X ◽  
Potato Leafroll Virus ◽  
First Report ◽  
Potato Virus A ◽  
Potato Viruses ◽  
The World ◽  
Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

scholarly journals Presence and distribution of economically important potato viruses in Montenegro

2011 ◽  
Vol 26 (2) ◽  
pp. 117-127
Author(s):  
Jelena Zindovic
Keyword(s):  
Potato Virus ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potato Leaf ◽  
Potato Virus S ◽  
Potato Varieties ◽  
Potato Viruses ◽  
Das Elisa

The research was carried out, in the period 2002-2004 in order to determine the presence and distribution of potato viruses at 12 different locations and on 9 different potato varieties grown in Montenegro. The research included collecting of samples in seed potato crops and testing of six economically important potato viruses: Potato leaf roll virus (PLRV), Potato virus Y (PVY), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) i Potato virus M (PVM). Using the direct enzyme-linked immunosorbent assay (DAS-ELISA) and commercial antisera specific for six potato viruses, it was found that PVY was the most frequent virus during the three-year research period. The second frequent virus was PVS, followed by PVA, PLRV, PVM and PVX. Single and mixed infections were detected, and the most prevalent were the single infections of PVY. Also, in the period 2002-2004, PVY had the highest distribution and the number of present viruses was different at different localities and on different potato varieties. Further investigations were related to detailed characterization of the most prevalent virus (PVY), which is at the same time economically the most important one. Serological characterization of PVY was performed utilizing DAS-ELISA kit with commercial monoclonal antibodies specific for detection of the three strain groups of PVY, and the two strain groups - necrotic (PVYN/PVYNTN) and common (PVYO), were identified. Necrotic strains were prevalent in 2002 and 2004, while in 2003 PVYO was the most frequent strain in virus population. The presence of stipple streak strain (PVYC) was not detected in any of the tested samples.

scholarly journals Identification and Molecular Characterization of Recombinant Potato Virus Y (PVY) in Potato from South Korea, PVYNTN Strain

Plant Disease ◽  
2019 ◽  
Vol 103 (1) ◽  
pp. 137-142 ◽  
Author(s):  
Mohamad Chikh-Ali ◽  
Mariana Rodriguez-Rodriguez ◽  
Kelsie J. Green ◽  
Dong-Jun Kim ◽  
Sang-Min Chung ◽  
...  
Keyword(s):  
South Korea ◽  
Whole Genome Sequencing ◽  
Molecular Characterization ◽  
Genome Sequencing ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Production ◽  
Whole Genome ◽  
Rt Pcr

Potato is an important source of food in South Korea, and viruses represent a significant threat to sustainable and profitable potato production. However, information about viruses affecting the potato crop in South Korea is limited. In 2017, potato plants of five cultivars exhibiting foliar mosaic, crinkling, and mottle were collected in two seed potato production areas, in Gangwon-do and Jeollabuk-do Provinces, and subjected to virus testing and characterization. Potato virus Y (PVY) was found associated with mosaic symptoms, and samples were characterized using reverse transcription polymerase chain reaction (RT-PCR) and whole genome sequencing. All analyzed PVY-positive samples were found to represent the same recombinant PVY strain: PVYNTN. Three PVY isolates were subjected to whole genome sequencing using overlapping RT-PCR fragments and Sanger methodology, and all three were confirmed to represent strain PVYNTNa after a recombination analysis of the complete genomes. In phylogenetic analysis, the three South Korean isolates were placed most closely to several PVYNTNa isolates reported from Japan and Vietnam, suggesting a common source of infection. This is the first report and complete molecular characterization of a PVYNTN strain present in the country, and because this strain induces tuber necrotic ringspot disease in susceptible cultivars of potato, appropriate management tools need to be implemented to mitigate potential tuber quality losses.

scholarly journals Molecular Characterization of Potato virus V Genomes from Europe Indicates Limited Spatiotemporal Strain Differentiation

2000 ◽  
Vol 90 (4) ◽  
pp. 437-444 ◽  
Author(s):  
Igor Oruetxebarria ◽  
Tuija Kekarainen ◽  
Carl Spetz ◽  
Jari P. T. Valkonen
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Genomic Sequence ◽  
Host Responses ◽  
Single Strain ◽  
Reading Frame ◽  
Solanaceae Species ◽  
Nontranslated Region

Because there were no previous reports on the molecular characterization of Potato virus V (PVV, genus Potyvirus, family Potyviridae), the complete genomic sequence of PVV isolate Dv42 was determined. The length of the single-stranded messenger-polarity RNA genome was 9,851 nt (nucleotides), followed by a poly(A) tail. The genome contained a 5′-terminal nontranslated region (5′-NTR; 204 nt), a single open reading frame (nucleotides 205-9406; 3,067 amino acids), and a 3′-NTR that was unusually long (446 nt) compared with that of Potato virus Y (PVY; 331-nt 3′-NTR), Potato virus A (PVA; 207-nt 3′-NTR), and other potyviruses that naturally infect Solanaceae species. Phylogenetic analysis with the cylindrical inclusion protein-encoding and coat protein (CP)-encoding regions indicated that PVV Dv42 was most closely related to Pepper mottle virus and PVY, respectively. Seven PVV isolates (including Dv42) collected from cultivated potatoes in the Netherlands, the United Kingdom, and Norway from 1964 to 1997 were uniform in serological properties and symptomatology in indicator hosts that could distinguish strains of PVY and PVA. The nucleotide sequences of the 5′-NTR, P1, CP, and 3′-NTR regions of the PVV isolates were determined and were 94.6 to 99.5, 96.3 to 98.8, 96.4 to 98.7, and 96.3 to 99.6% identical, respectively. The amino acid similarities for the P1 and CP were 95.8 to 98.6 and 96.0 to 97.8%, respectively. Phylogenetic analysis of the CP sequences of PVV revealed no significant grouping, in contrast to PVY and PVA, which were grouped largely according to the previously recognized strains based on host responses. However, the relatively few differences in the P1 sequences of PVV were correlated with the different countries of origin. Hence, the PVV isolates infecting potatoes in Europe seem to vary little genetically and may belong to a single strain.

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