The Occurrence of Potato Viruses in Syria and the Molecular Detection and Characterization of Syrian Potato virus S Isolates

The research was carried out, in the period 2002-2004 in order to determine the presence and distribution of potato viruses at 12 different locations and on 9 different potato varieties grown in Montenegro. The research included collecting of samples in seed potato crops and testing of six economically important potato viruses: Potato leaf roll virus (PLRV), Potato virus Y (PVY), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) i Potato virus M (PVM). Using the direct enzyme-linked immunosorbent assay (DAS-ELISA) and commercial antisera specific for six potato viruses, it was found that PVY was the most frequent virus during the three-year research period. The second frequent virus was PVS, followed by PVA, PLRV, PVM and PVX. Single and mixed infections were detected, and the most prevalent were the single infections of PVY. Also, in the period 2002-2004, PVY had the highest distribution and the number of present viruses was different at different localities and on different potato varieties. Further investigations were related to detailed characterization of the most prevalent virus (PVY), which is at the same time economically the most important one. Serological characterization of PVY was performed utilizing DAS-ELISA kit with commercial monoclonal antibodies specific for detection of the three strain groups of PVY, and the two strain groups - necrotic (PVYN/PVYNTN) and common (PVYO), were identified. Necrotic strains were prevalent in 2002 and 2004, while in 2003 PVYO was the most frequent strain in virus population. The presence of stipple streak strain (PVYC) was not detected in any of the tested samples.

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Molecular Characterization of Potato leafroll virus, Potato virus A, and Potato virus X Isolates from Potatoes in Alaskan Cities and Villages

Plant Health Progress ◽

10.1094/php-2011-0209-01-br ◽

2011 ◽

Vol 12 (1) ◽

pp. 39 ◽

Cited By ~ 2

Author(s):

Nancy L. Robertson ◽

Jeffrey Smeenk ◽

Jodie M. Anderson

Keyword(s):

Molecular Characterization ◽

Potato Virus ◽

Potato Virus X ◽

Potato Leafroll Virus ◽

First Report ◽

Potato Virus A ◽

Potato Viruses ◽

The World ◽

Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

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Strain Characterization of Potato virus S Isolates from Tasmania, Australia

Plant Disease ◽

10.1094/pdis-07-11-0573 ◽

2012 ◽

Vol 96 (6) ◽

pp. 813-819 ◽

Cited By ~ 11

Author(s):

Susan J. Lambert ◽

Jason B. Scott ◽

Sarah J. Pethybridge ◽

Frank S. Hay

Keyword(s):

Potato Virus ◽

Chenopodium Quinoa ◽

Protein Sequencing ◽

Potato Seed ◽

Serological Detection ◽

Potato Virus S ◽

Strain Characterization ◽

Geographical Regions ◽

Local Lesions

Potato virus S (PVS) is prevalent within potato (Solanum tuberosum) production worldwide. Traditionally, PVS has been split into two strains, Ordinary (PVSO) and Andean (PVSA), based on reaction in herbaceous indicator species such as Chenopodium quinoa. However, recent research has identified further strain designations, such as PVSO-CS (Ordinary and Chenopodium systemic). Forty-four isolates of PVS were collected from potato seed lines in different geographical regions within Tasmania, Australia. Isolates were initially characterized by reactions in C. quinoa. Nineteen isolates were characterized as PVSO, based on the development of local lesions and serological detection in inoculated leaves only. Three isolates were identified as PVSA-like, based on local lesion development in inoculated leaves, mild mottling or chlorotic spots on noninoculated leaves, and serological detection in both inoculated and noninoculated leaves. Thirteen isolates produced no symptoms, and were detected serologically in inoculated leaves only (PVSO-like). Four isolates produced no symptoms but were detected serologically in both inoculated and noninoculated leaves (PVSA-like). Five isolates produced symptoms in inoculated leaves only but were detected serologically in both inoculated and noninoculated leaves (also PVSA-like). The ability of isolates to infect tomato has also been used as a criterion to assist in PVS strain differentiation. A subsample of isolates (n = 16) was unable to infect tomato ‘Grosse Lisse’. Seventeen isolates representative of these groupings based on reactions in C. quinoa were also characterized by coat-protein sequencing. Phylogenetic comparisons suggested that all isolates were PVSO rather than PVSA. Therefore, whereas some of these PVS isolates were systemic in C. quinoa, findings from this study suggest that they were not PVSA, and that only PVSO and PVSO-CS isolates are present in Tasmania. The implications of this finding for disease management are discussed.

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Prevalence, distribution and control of six major potato viruses in Kenya

Tropical Plant Pathology ◽

10.1007/s40858-020-00409-x ◽

2020 ◽

Author(s):

John Onditi ◽

Moses Nyongesa ◽

René van der Vlugt

Keyword(s):

Potato Virus ◽

Leaf Roll ◽

Potato Virus X ◽

Plant Viruses ◽

Multiple Infections ◽

Potato Seed ◽

Potato Leaf ◽

Potato Virus S ◽

Potato Viruses ◽

Virus Prevalence

AbstractIn most developing countries, farmers lack sufficient supply of certified or healthy potato seed tubers. Hence, they often plant their own saved ware potato tubers, a practice that is known to contribute to spread and increase the prevalence of plant viruses. In this study, we proposed options for managing the virus based on the knowledge obtained from surveys of virus prevalence and distribution in potato cultivars grown under such conditions. Potato leaf samples randomly collected from 354 farms in five major potato-growing counties in Kenya were tested for six potato viruses; potato virus Y (PVY), potato leaf roll virus (PLRV), potato virus X (PVX), potato virus M (PVM), potato virus A (PVA) and potato virus S (PVS) through DAS-ELISA. Virus prevalence in the fields was high; 72.9% of the samples were positive for at least one of the six viruses; and 55.9% showed multiple infections. A follow-up survey conducted during three consecutive seasons, in two of the five counties, revealed that virus prevalence fluctuated across seasons. This suggested that updated information on virus prevalence might be of value for designing a virus control strategy. Distribution maps showed the presence of the viruses restricted to specific geographic regions, an indication of where control efforts should be directed. Four cultivars, Sherekea, Shangi, Kenya Karibu and Asante, grown at a high virus-prevalent area, showed low values of average ELISA absorbance (OD), suggest a field resistance to the viruses. This study demonstrated that knowledge of prevalence and distribution may be of value to identify and recommend virus resistant cultivars to replace susceptible ones, especially in the virus hotspot areas.

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Effectiveness of Positive Selection in Managing Seed-Borne Potato Viruses

Journal of Agricultural Science ◽

10.5539/jas.v10n3p71 ◽

2018 ◽

Vol 10 (3) ◽

pp. 71

Author(s):

Gidraf Onduru Okeyo ◽

Kalpana Sharma ◽

Elly Atieno ◽

Rama Devi Narla ◽

Douglas Watuku Miano ◽

...

Keyword(s):

Positive Selection ◽

Potato Virus ◽

Leaf Roll ◽

Multiple Infections ◽

Potato Leaf ◽

Potato Virus S ◽

Infected Seed ◽

Potato Viruses ◽

Field Generation ◽

Seed Tubers

Potato productivity in Kenya has remained below the potential due to the use of infected seed tubers especially by seed borne viruses. A study was carried out to asses the effectiveness of Positive Selection (PS) in managing seed borne potato viruses. Sprouted seed potato tubers of nine genotypes; three commercial varieties and six advanced International Potato Center (CIP) clones were screened in the field for two seasons, Field Generation Three (FG3) and Field Generation Four (FG4), respectively. In each season, experimental plots were divided into two sub-plots in which one sub-plot was planted with seed tubers sourced through Random Seed Selection (RSS) and the other with seed tuber sourced through Positive Selection (PS). Positive selection plots had low visual virus incidences, high number of tubers per hill and yield (t/ha) in both FG3 and FG4 growing seasons irrespective of the genotypes. ELISA test also revealed that Potato Virus S (PVS) (78%) was the most predominant virus followed by Potato Virus Y (PVY) (20%), Potato Leaf Roll Virus (PLRV) (16%) and Potato Virus M (PVM) (9%) in RSS plots either with single or multiple infections. Positive Selection plots recorded low virus incidences of PVS (47%), and PVY, PLRV and PVM were absent in the tubers tested with ELISA. This study concludes that Positive Selection is a good management strategy to manage the seed borne potato viruses, and if adopted, small holder farmers can reduce the yield losses arising from use of virus infected seed tubers.

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Status of potato viruses in Tunisia and molecular characterization of Tunisian Potato Virus X (PVX) isolates

European Journal of Plant Pathology ◽

10.1007/s10658-017-1407-2 ◽

2017 ◽

Vol 151 (3) ◽

pp. 735-744 ◽

Cited By ~ 1

Author(s):

Ahmed Ben Hafsa ◽

Nesrine Nabi ◽

Besma M’rabet Saamali ◽

Mohammed Salem Zellama ◽

Sébastien Theil ◽

...

Keyword(s):

Molecular Characterization ◽

Potato Virus ◽

Potato Virus X ◽

Potato Viruses

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Molecular Detection of Potato Viruses in Bangladesh and Their Phylogenetic Analysis

Plants ◽

10.3390/plants9111413 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1413

Author(s):

Mamun-Or Rashid ◽

Ying Wang ◽

Cheng-Gui Han

Keyword(s):

Phylogenetic Analysis ◽

Potato Virus ◽

Control Strategies ◽

Potato Virus X ◽

Potato Production ◽

Chinese Isolate ◽

Potato Virus S ◽

Iranian Isolate ◽

Potato Viruses ◽

Canadian Isolate

Potato (Solanum tuberosum) is a major food source in the whole world including Bangladesh. Viral diseases are the key constraint for sustainable potato production by reducing both quality and quantity. To determine the present status of eight important potato viruses in Bangladesh, tuber samples were collected from three major potato growing regions (Munshiganj, Jessore and Bogra districts) in January–February 2017 and February 2018. Reverse transcription polymerase chain reaction (RT-PCR) with coat protein (CP)-specific primers were used to amplify CP sequences of the respective viruses, and confirmed by sequencing, which were deposited in the GenBank. Results indicated that the tuber samples were subjected to Potato leafroll virus (PLRV), Potato virus X (PVX), Potato virus Y (PVY), Potato virus S (PVS), Potato virus H (PVH), Potato aucuba mosaic virus (PAMV) and Potato virus M (PVM) infection, whereas mixed infections were very common. Phylogenetic analysis revealed that the PLRV from this study was closely related to a Canadian and a Chinese isolate, respectively; PVX was closely related to a Canadian and a Chinese isolate, respectively; PVY was closely related to a Chinese isolate; PVS was closely related to a Chinese and an Iranian isolate, respectively; PAMV was closely related to a Canadian isolate; PVH was closely related to a Huhhot isolate of China; and PVM was closely related to an Indian and an Iranian isolate, respectively. As far as we know, PAMV in this study is the first report in Bangladesh. These findings will provide a great scope for appropriate virus control strategies to virus free potato production in Bangladesh.

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