In vitro effects of binuclear (η 6-p-cymene)ruthenium(II) complex containing bridging bis(nicotinate)-polyethylene glycol ester ligand on differentiation pathways of murine Th lymphocytes activated by T cell mitogen

2015 ◽  
Vol 20 (3) ◽  
pp. 575-583 ◽  
Author(s):  
Miljana Momcilovic ◽  
Thomas Eichhorn ◽  
Jana Blazevski ◽  
Harry Schmidt ◽  
Goran N. Kaluđerović ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
T Cell ◽  
Glycol Ester ◽  
In Vitro Effects ◽  
Cell Mitogen ◽  
Differentiation Pathways ◽  
Th Lymphocytes

scholarly journals The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. III. B-cell dependence for T-cell proliferation.

1979 ◽  
Vol 149 (5) ◽  
pp. 1001-1017 ◽  
Author(s):  
M K Ho ◽  
A S Kong ◽  
S I Morse
Keyword(s):  
T Cell ◽  
T Cell Proliferation ◽  
Complement Receptors ◽  
Surface Immunoglobulin ◽  
Helper Cells ◽  
Mouse Immunoglobulin ◽  
In Vitro Effects ◽  
Cell Mitogen ◽  
Stimulation Of

The nature of the helper lymphocytes in lymphocytosis-promoting factor (LPF)-induced proliferation was explored. Removal of macrophages from adherent splenocytes by either carbonyl-iron incubation or passage through Sephadex G-10 columns did not affect their synergistic function. Nor did cytolysis with Thy-1.2 antiserum and complement. The helper cells were found to be surface immunoglobulin-positive (sIg+) because they are retained by anti-Ig columns, susceptible to lysis by rabbit anti-mouse immunoglobulin and complement, and occurred in the sIg+ fractions of splenocytes after separation on the fluorescence-activated cell sorter. Further delineation of the surface markers on helper cells showed that complement receptors are not the determining marker for synergistic function. The requirement for B-helper cells in the stimulation of T lymphocytes by LPF is unique for a mouse of T-cell mitogen.

In Vitro Effects of Cyclosporine A and Tacrolimus on Regulatory T-Cell Proliferation and Function

2012 ◽  
Vol 94 (2) ◽  
pp. 123-131 ◽  
Author(s):  
Céline Miroux ◽  
Olivier Morales ◽  
Khaldoun Ghazal ◽  
Samia Ben Othman ◽  
Yvan de Launoit ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cell ◽  
Cyclosporine A ◽  
Regulatory T Cell ◽  
T Cell Proliferation ◽  
In Vitro Effects ◽  
And Function

In Vitro Effects of Aminobisphosphonates on Vγ9Vδ2 T Cell Activation and Differentiation

2006 ◽  
Vol 19 (2) ◽  
pp. 309-317 ◽  
Author(s):  
V. Ferlazzo ◽  
C. Sferrazza ◽  
N. Caccamo ◽  
G. Di Fede ◽  
G. Di Lorenzo ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
In Vitro Effects

scholarly journals In Vivo and In Vitro Effects of Antituberculosis Treatment on Mycobacterial Interferon-γ T Cell Response

PLoS ONE ◽  
2009 ◽  
Vol 4 (4) ◽  
pp. e5187 ◽  
Author(s):  
Ilaria Sauzullo ◽  
Fabio Mengoni ◽  
Miriam Lichtner ◽  
Anna Paola Massetti ◽  
Raffaella Rossi ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Response ◽  
Interferon Γ ◽  
T Cell Response ◽  
Antituberculosis Treatment ◽  
In Vitro Effects

OC.07.3 IN VITRO EFFECTS OF MESENCHYMAL STROMAL CELLS (MSC) ON T CELL LINES FROM CROHN'S DISEASE PATIENTS

2013 ◽  
Vol 45 ◽  
pp. S71
Author(s):  
R. Ciccocioppo ◽  
G.C. Cangemi ◽  
E. Betti ◽  
A. Gallia ◽  
V. Imbesi ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
T Cell ◽  
Crohn's Disease ◽  
Cell Lines ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
In Vitro Effects ◽  
T Cell Lines

scholarly journals IMMU-48. EVALUATING THE IN-VITRO EFFECTS OF TUMOR TREATING FIELDS ON T-CELL RESPONSES

2017 ◽  
Vol 19 (suppl_6) ◽  
pp. vi123-vi123
Author(s):  
Gil Diamant ◽  
Zvi Ram ◽  
Ilan Volovitz
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Cell Responses ◽  
Tumor Treating Fields ◽  
In Vitro Effects

scholarly journals Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients

2008 ◽  
Vol 2008 ◽  
pp. 1-10 ◽  
Author(s):  
Stefanie Kuerten ◽  
Tobias R. Schlingmann ◽  
Tarvo Rajasalu ◽  
Doychin N. Angelov ◽  
Paul V. Lehmann ◽  
...  
Keyword(s):  
T Cell ◽  
False Positive ◽  
T Cell Responses ◽  
Costimulatory Molecules ◽  
Healthy Individuals ◽  
Low Frequencies ◽  
Cell Responses ◽  
Disease Specificity ◽  
In Vitro Effects

Measurements of antigen-specific T cell responses in chronic diseases are limited by low frequencies of antigen-specific cells in the peripheral blood. Therefore, attempts have been made to add costimulatory molecules such as anti-CD28 or IL-7/IL-15 to ELISPOT assays to increase sensitivity. While this approach has been successful under certain circumstances, results are often inconsistent. To date, there are no comprehensive studies directly comparing the in vitro effects of multiple costimulatory molecules in different disease settings. Therefore, in the present study we tested the effects of IL-7/IL-15, IFN-α, anti-ICOS, and anti-CD28 on antigen-specific T cell responses in patients infected with HCV or HIV versus healthy individuals. Our data show that none of the aforementioned molecules could significantly increase ELISPOT sensitivity, neither in HCV nor in HIV. Moreover, all of them caused false-positive responses to HCV and HIV antigens in healthy individuals. Our results question the broad use of in vitro costimulation.

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