Preparation and characterization of Jagged1-bound fibrinogen-based microspheres and their cytotoxicity against human dental pulp cells

2020 ◽  
Vol 34 (8) ◽  
pp. 1105-1113
Author(s):  
Chawan Manaspon ◽  
Lawan Boonprakong ◽  
Thantrira Porntaveetus ◽  
Thanaphum Osathanon

Surface immobilization of Jagged1 promotes odonto/osteogenic differentiation in human dental pulp cells. On the contrary, soluble Jagged1 fails to activate target gene expression of Notch signaling which is important for differentiation of human dental pulp cells. Hence, Jagged1 delivery system is indeed required for transportation of immobilized Jagged1 to promote odontogenic differentiation of human dental pulp cells in vivo. The present study described the preparation and characterization of Jagged1-bound fibrinogen-based microspheres. Water-in-oil emulsion technique was employed to prepare fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres. The average size of fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres was 213.9 ± 35.9 and 199.9 ± 41.9 µm, respectively. These microspheres did not alter the human dental pulp cells’ cell viability. Human dental pulp cells were able to attach and spread on these microspheres. Jagged1 was conjugated on microspheres using 1-ethyl-3-(3-dimethylamino) propyl carbodiimide/N-hydroxysuccinimide. Binding capacity of Jagged1 on both fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres ranged from 25.8 ± 6.0 to 35.6 ± 9.1%. There was no significant difference in the size of microspheres between before and after Jagged1 conjugation process. In conclusion, fibrinogen microspheres and thrombin cross-linked fibrinogen microspheres could be utilized as the alternative biomaterials for Jagged1 delivery for future biomedical application.

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2449
Author(s):  
Beata Kaczmarek-Szczepańska ◽  
Olha Mazur ◽  
Marta Michalska-Sionkowska ◽  
Krzysztof Łukowicz ◽  
Anna Maria Osyczka

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.


2018 ◽  
Author(s):  
Sakiko Fujii ◽  
Katsumi Fujimoto ◽  
Noriko Goto ◽  
Yoshimitsu Abiko ◽  
Asayo Imaoka ◽  
...  

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 193 ◽  
Author(s):  
Chang Youp Ok ◽  
Sera Park ◽  
Hye-Ock Jang ◽  
Takashi Takata ◽  
Moon-Kyoung Bae ◽  
...  

Dental pulp plays an important role in the health of teeth. The aging of teeth is strongly related to the senescence of dental pulp cells. A novel adipokine, visfatin, is closely associated with cellular senescence. However, little is known about the effect of visfatin on the senescence of human dental pulp cells (hDPCs). Here, it was found that in vivo visfatin levels in human dental pulp tissues increase with age and are upregulated in vitro in hDPCs during premature senescence activated by H2O2, suggesting a correlation between visfatin and senescence. In addition, visfatin knockdown by small interfering RNA led to the reduction in hDPC senescence; however, treatment with exogenous visfatin protein induced the senescence of hDPCs along with increased NADPH consumption, which was reversed by FK866, a chemical inhibitor of visfatin. Furthermore, visfatin-induced senescence was associated with both the induction of telomere damage and the upregulation of senescence-associated secretory phenotype (SASP) factors as well as NF-κB activation, which were all inhibited by FK866. Taken together, these results demonstrate, for the first time, that visfatin plays a pivotal role in hDPC senescence in association with telomere dysfunction and the induction of SASP factors.


2007 ◽  
Vol 52 (8) ◽  
pp. 727-731 ◽  
Author(s):  
Masae Kitagawa ◽  
Hirota Ueda ◽  
Sinji Iizuka ◽  
Kiyako Sakamoto ◽  
Hiroko Oka ◽  
...  

Human Cell ◽  
2020 ◽  
Vol 33 (3) ◽  
pp. 490-501
Author(s):  
Tomoko Kobayashi ◽  
Daisuke Torii ◽  
Takanori Iwata ◽  
Yuichi Izumi ◽  
Masanori Nasu ◽  
...  

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