Peroxiredoxin I expression in epithelial cells of buccal mucosa from patients exposed to panoramic X-rays: influence of the age

Abstract Thymocytes are genuine lymphocytes. The largest lymphocytes of lymph nodes do not occur in the thymus; the smallest ones with pacychromatic nuclei and scanty cytoplasm are more numerous in the thymus. Some lymphoblasts similar to myeloblasts occur in the rabbit thymus. All three types of granulocytes develop from lymphocytes and to some extent from the mesenchymatous reticulum in the rabbit. In the rabbit the heterophil and eosinophil granules are acidophilic when first formed, while in the human thymus many have a basophilic quota as in the marrow. The reticulum is largely of epithelial origin and retains its epithelial characteristics in cytoplasm and nucleus. In the rabbit some mesenchymatous tissue is blended with the reticulum and may give origin to lymphocytes and granulocytes. The epithelium may form macrophages but does not differentiate to lymphocytes and other types of cells. In imprints from the rabbit the epithelial cells usually can be distinguished from those of the mesenchymatous reticulum. During regeneration following irradiation the latter tissue can be seen to form some lymphocytes locally; other lymphocytes enter the organ through the lymph vessels. Imprints stained with May-Grünwald and Giemsa are of great value for detailed cytology of the thymus and for determining cell relationships. Sections of thymus fixed in Helly’s fluid, stained with methyl green and pyronin and dehydrated in dioxan are excellent for lymphocytes and the transitional stages resulting from their development from fixed mesenchymatous tissue during regeneration of the organ following exposure to x-rays.

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Relationship between cytological features of buccal epithelial cells and biochemical parameters in obese schoolchildren

Voprosy detskoj dietologii ◽

10.20953/1727-5784-2021-1-72-77 ◽

2021 ◽

Vol 19 (1) ◽

pp. 72-77

Author(s):

O.P. Kovtun ◽

◽

V.V. Bazarnyi ◽

E.V. Anufrieva ◽

L.G. Polushina ◽

...

Keyword(s):

Lipid Metabolism ◽

Epithelial Cells ◽

Buccal Mucosa ◽

Chromatin Condensation ◽

Healthy Children ◽

Obese Children ◽

Cross Sectional ◽

Binucleated Cells ◽

Buccal Epithelial Cells ◽

Cytological Characteristics

Objective. To determine the relationship between metabolic parameters and cytological characteristics of the buccal mucosa in obese children. Patients and methods. An open cross-sectional case-control study included 78 schoolchildren aged 9 to 15 years, 44 of them were obese. We determined body mass index, lipid metabolism parameters (total cholesterol, high- and low-density lipoprotein cholesterol, apolipoproteins), and also cytological characteristics of buccal epithelial cells. Results. The number of buccal epithelial cells with condensed chromatin was by 10 times higher in obese children. A correlation was found between cholesterol concentrations and certain cytological parameters, but this correlation was statistically significant only in the pair “cholesterol – number of binucleated cells” (correlation coefficient 0.925, p = 0.05) in obese children and in the pair “total protein – binucleated cells” in practically healthy children. Conclusion. We confirmed the known data about specific characteristics of lipid metabolism in obese children that correlate with certain cytological parameters of the buccal mucosa, namely, an increased number of cells with chromatin condensation. This phenomenon is suggestive of activation of apoptosis in obese children. Keywords: buccal epithelial cells, obesity, schoolchildren, cholesterol

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Genotoxic and cytotoxic effects of Haas appliance in exfoliated buccal mucosa cells during orthodontic treatment

The Angle Orthodontist ◽

10.2319/101117-687.1 ◽

2018 ◽

Vol 88 (5) ◽

pp. 590-595 ◽

Cited By ~ 1

Author(s):

Arthur S. Cunha ◽

Willian Orlando Castillo ◽

Catarina Satie Takahashi ◽

Erika Calvano Küchler ◽

Raquel Assed Bezerra Segato ◽

...

Keyword(s):

Epithelial Cells ◽

Buccal Mucosa ◽

Orthodontic Treatment ◽

Nonparametric Tests ◽

Cytotoxic Effects ◽

Significance Level ◽

Posterior Crossbite ◽

Cellular Abnormalities ◽

Variance Testing ◽

The Right

ABSTRACT Objectives: To evaluate the genotoxic and cytotoxic effects of Haas appliances through micronuclei test and cytogenetic damage analysis in buccal mucosa epithelial cells of patients undergoing orthodontic treatment. Materials and Methods: Twenty-eight patients, 6–12 years of age and of both genders, who required a Haas appliance for the correction of a posterior crossbite were included. Epithelial cells from the mucosa were collected by gently scraping the inside of both the right and left cheeks. The cells were collected before the insertion of the appliance (T0), 1 month after the device was installed (T1), and again 3 months after the appliance was immobilized (T2). The cells were processed to obtain slides. Feulgen/Fast Green was used as the staining method, and the number of normal, karyolytic, pyknotic, nuclear buds, bi/trinucleated, and micronucleus cells were counted under light microscopy. Cellular abnormalities were evaluated with parametric and nonparametric tests for comparison of the means by analysis of variance testing, Tukey posttest, or the Kruskal-Wallis test and then by Dunn's posttest. The significance level was 5%. Results: There were no statistically significant changes in the micronuclei in the evaluated periods (P > .05). Nuclear buds increased at T1 (P < .05), returning to baseline levels at T2. Other abnormalities (cariolytic, pyknotic, and bi/trinucleated cells) showed a significant increase at T1 and T2 (P < .0001). Conclusions: The Haas appliance did not cause an increase in micronuclei in cells of the buccal mucosa. However, statistically significant increases in cariolytic, pyknotic, and bi/trinucleated cells were observed during treatment, suggesting possible DNA damage.

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