scholarly journals Bacterial translocation and microgap formation at a novel conical indexed implant abutment system for single crowns

2021 ◽  
Author(s):  
Peter Gehrke ◽  
Simon Burg ◽  
Ulrike Peters ◽  
Thomas Beikler ◽  
Carsten Fischer ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sem Analysis ◽  
Electron Microscopic ◽  
Qrt Pcr ◽  
Implant Abutment ◽  
Bacterial Migration ◽  
Cad Cam ◽  
Bacterial Leakage ◽  
Single Crowns

Abstract Objectives A conometric concept was recently introduced in which conical implant abutments hold the matching crown copings by friction alone, eliminating the need for cement or screws. The aim of this in vitro study was to assess the presence of microgap formation and bacterial leakage at the Acuris conometric restorative interface of three different implant abutment systems. Material and methods A total of 75 Acuris samples of three implant-abutment systems (Ankylos, Astra Tech EV, Xive) were subjected to microbiological (n = 60) and scanning electron microscopic (SEM) investigation (n = 15). Bacterial migration into and out of the conical coupling system were analyzed in an anaerobic workstation for 48, 96, 144, and 192 h. Bacterial DNA quantification using qrt-PCR was performed at each time point. The precision of the conometric coupling and internal fit of cemented CAD/CAM crowns on corresponding Acuris TiN copings were determined by means of SEM. Results qrt-PCR results failed to demonstrate microbial leakage from or into the Acuris system. SEM analysis revealed minute punctate microgaps at the apical aspect of the conometric junction (2.04 to 2.64 µm), while mean cement gaps of 12 to 145 µm were observed at the crown-coping interface. Conclusions The prosthetic morse taper connection of all systems examined does not allow bacterial passage. Marginal integrity and internal luting gap between the ceramic crown and the coping remained within the clinically acceptable limits. Clinical relevance Conometrically seated single crowns provide sufficient sealing efficiency, relocating potential misfits from the crown-abutment interface to the crown-coping interface.

scholarly journals Marginal Adaptation and Microbial Leakage at Conometric Prosthetic Connections for Implant-Supported Single Crowns: An In Vitro Investigation

2021 ◽  
Vol 22 (2) ◽  
pp. 881
Author(s):  
Peter Gehrke ◽  
Philip Hartjen ◽  
Ralf Smeets ◽  
Martin Gosau ◽  
Ulrike Peters ◽  
...  
Keyword(s):  
Anaerobic Bacteria ◽  
In Vitro Study ◽  
Clinical Results ◽  
Test System ◽  
Sem Analysis ◽  
Blood Agar ◽  
Qrt Pcr ◽  
In Vitro Investigation ◽  
Single Crowns

Encouraging clinical results were reported on a novel cone-in-cone coupling for the fixation of dental implant-supported crowns (Acuris, Dentsply Sirona Implants, Mölndal, Sweden). However, the presence or absence of a microgap and a potential bacterial leakage at the conometric joint has not yet been investigated. A misfit and a resulting gap between the conometric components could potentially serve as a bacterial reservoir that promotes plaque formation, which in turn may lead to inflammation of the peri-implant tissues. Thus, a two-fold study set-up was designed in order to evaluate the bidirectional translocation of bacteria along conometrically seated single crowns. On conometric abutments filled with a culture suspension of anaerobic bacteria, the corresponding titanium nitride-coated (TiN) caps were fixed by friction. Each system was sterilized and immersed in culture medium to provide an optimal environment for microbial growth. Positive and negative controls were prepared. Specimens were stored in an anaerobic workstation, and total and viable bacterial counts were determined. Every 48 h, samples were taken from the reaction tubes to inoculate blood agar plates and to isolate bacterial DNA for quantification using qrt-PCR. In addition, one Acuris test system was subjected to scanning electron microscopy (SEM) to evaluate the precision of fit of the conometric coupling and marginal crown opening. Throughout the observational period of one week, blood agar plates of the specimens showed no viable bacterial growth. qrt-PCR, likewise, yielded a result approaching zero with an amount of about 0.53 × 10−4 µg/mL DNA. While the luting gap/marginal opening between the TiN-cap and the ceramic crown was within the clinically acceptable range, the SEM analysis failed to identify a measurable microgap at the cone-in-cone junction. Within the limits of the in-vitro study it can be concluded that the Acuris conometric interface does not allow for bacterial translocation under non-dynamic loading conditions.

scholarly journals Effect of Fabrication Technique on the Microgap of CAD/CAM Cobalt–Chrome and Zirconia Abutments on a Conical Connection Implant: An In Vitro Study

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2348
Author(s):  
Pedro Molinero-Mourelle ◽  
Rocio Cascos-Sanchez ◽  
Burak Yilmaz ◽  
Walter Yu Hang Lam ◽  
Edmond Ho Nang Pow ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sem Analysis ◽  
Vitro Study ◽  
Fabrication Technique ◽  
Chewing Simulation ◽  
Implant Abutment ◽  
Conical Connection ◽  
Cad Cam ◽  
Pair Wise Comparison

The aim of this in vitro study was to investigate the microgaps at the implant–abutment interface when zirconia (Zr) and CAD/CAM or cast Co–Cr abutments were used. Methods: Sixty-four conical connection implants and their abutments were divided into four groups (Co–Cr (milled, laser-sintered and castable) and Zirconia (milled)). After chewing simulation (300,000 cycles, under 200 N loads at 2 Hz at a 30° angle) and thermocycling (10,000 cycles, 5 to 50 °C, dwelling time 55 s), the implant–abutment microgap was measured 14 times at each of the four anatomical aspects on each specimen by using a scanning electron microscope (SEM). Kruskal–Wallis and pair-wise comparison were used to analyze the data (α = 0.05). Results: The SEM analysis revealed smaller microgaps with Co–Cr milled abutments (0.69–8.39 μm) followed by Zr abutments (0.12–6.57 μm), Co–Cr sintered (7.31–25.7 μm) and cast Co–Cr (1.68–85.97 μm). Statistically significant differences were found between milled and cast Co–Cr, milled and laser-sintered Co–Cr, and between Zr and cast and laser-sintered Co–Cr (p < 0.05). Conclusions: The material and the abutment fabrication technique affected the implant–abutment microgap magnitude. The Zr and the milled Co–Cr presented smaller microgaps. Although the CAD/CAM abutments presented the most favorable values, all tested groups had microgaps within a range of 10 to 150 μm.

scholarly journals Association between implant abutment microgap and implant circularity to bacterial leakage - an in vitro study using tapered connection implants

2018 ◽  
Vol 29 ◽  
pp. 294-294
Author(s):  
Eduardo Claudio Lopes de Chaves E. Mello Dias ◽  
Marcelo Sperandio ◽  
Marcelo Henrique Napimoga
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Implant Abutment ◽  
Bacterial Leakage ◽  
Tapered Connection

scholarly journals Microbiological Sealing Analysis of a Tapered Connection and External Hexagon System

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Gardel Nepomuceno Costa ◽  
Elizabeth Ferreira Martinez ◽  
Aluísio Martins de Oliveira Ruellas ◽  
Daiane Cristina Peruzzo ◽  
Júlio Cesar Joly ◽  
...  
Keyword(s):  
Descriptive Analysis ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Microbiological Analysis ◽  
Significance Level ◽  
Implant Abutment ◽  
Bacterial Leakage ◽  
Tapered Connection

Considering the variety of implant connection systems available in the market and the contrasting literature regarding tapered connection systems in terms of bacterial leakage, the aim of this in vitro study was to compare the effectiveness of the bacterial seal at the implant/abutment interface between an external hexagon and a tapered connection system. Twelve sets of indexed tapered connection components and twelve sets of external hexagon connection components were used for microbiological analysis. In addition, for each model, an implant with its respective prosthetic abutment was used as a negative control and another as a positive control of microbial contamination. Failure of the abutment/implant interface seal was observed via turbidity or presence of deposits in the culture. Descriptive analysis of the data and relative frequency (percentage) as well as Fisher’s exact test were used at a significance level of 5%. Two of ten (20%) external hexagon specimens showed contamination against 0/10 (0%) tapered connection implants. In conclusion, both implant/abutment connections were able to prevent bacterial leakage in vitro.

Bacterial Leakage in Implants With Different Implant–Abutment Connections: An In Vitro Study

2012 ◽  
Vol 83 (4) ◽  
pp. 491-497 ◽  
Author(s):  
Bartolomeo Assenza ◽  
Domenico Tripodi ◽  
Antonio Scarano ◽  
Vittoria Perrotti ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Implant Abutment ◽  
Bacterial Leakage
Sign in / Sign up

Export Citation Format

Share Document