In silico studies on the interaction of phage displayed biorecognition element (TFQAFDLSPFPS) with the structural protein VP28 of white spot syndrome virus

Abstract White Spot Syndrome Virus (WSSV) infects several economically important aquacultural species, causing significant losses to the industry. This virus belongs to the Nimaviridae family, and has a dsDNA genome ranging from 257 to 309 kb (more than 20 isolate genomes fully sequenced and published to date). Multiple routes of infection could be the cause of the high virulence and mortality rates detected in shrimp species. In particular, Penaeus vannamei, differences in isolate virulence have been observed, along with controversy over whether deletions or insertions are associated with gain or loss of virulence.The pathogenicity of three isolates from three locations in Mexico (two from Sinaloa: 'CIAD', and 'Angostura', and one from Sonora: 'Sonora') was evaluated in vivo in white shrimp (P. vannamei) infection assays. Differences were observed in the mortality rate of shrimp among the three isolates, with the Sonora isolate being the most virulent. Subsequently, the complete WSSV genomes were sequenced in depth from the tissues of infected shrimp, and assembled in reference to the genome of isolate CN01 (KT995472), identifying genome sizes for Angostura and Sonora of 289,350 bp and 288,995 bp, respectively. Where three deletion zones were identified compared to CN01 comprising 15 genes, including three envelope proteins VP41A, VP52A and VP41B, one non-structural protein ICP35 and 11 others encoding proteins whose function is currently unknown. In addition, five genes (wsv129, wsv178, wsv204, wsv249 and wsv497) show a modified number of repeat motifs. The main implications and possible effects on viral infection of these modifications are discussed.

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Polycistronic mRNAs and internal ribosome entry site elements (IRES) are widely used by white spot syndrome virus (WSSV) structural protein genes

Virology ◽

10.1016/j.virol.2009.02.012 ◽

2009 ◽

Vol 387 (2) ◽

pp. 353-363 ◽

Cited By ~ 14

Author(s):

Shih-Ting Kang ◽

Jiann-Horng Leu ◽

Han-Ching Wang ◽

Li-Li Chen ◽

Guang-Hsiung Kou ◽

...

Keyword(s):

Internal Ribosome Entry Site ◽

White Spot Syndrome Virus ◽

White Spot ◽

Structural Protein ◽

Entry Site ◽

Internal Ribosome Entry ◽

White Spot Syndrome

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High sensitivity immunochromatographic strip test (ICP11 strip test) for white spot syndrome virus detection using monoclonal antibodies specific to ICP11 non-structural protein

Aquaculture ◽

10.1016/j.aquaculture.2016.12.004 ◽

2017 ◽

Vol 470 ◽

pp. 25-31 ◽

Cited By ~ 5

Author(s):

Pradit Wangman ◽

Ruthairat Siriwattanarat ◽

Siwaporn Longyant ◽

Chalinan Pengsuk ◽

Paisarn Sithigorngul ◽

...

Keyword(s):

Monoclonal Antibodies ◽

White Spot Syndrome Virus ◽

Virus Detection ◽

High Sensitivity ◽

White Spot ◽

Structural Protein ◽

Immunochromatographic Strip ◽

Immunochromatographic Strip Test ◽

White Spot Syndrome ◽

Strip Test

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Genomic and Proteomic Analysis of Thirty-Nine Structural Proteins of Shrimp White Spot Syndrome Virus

Journal of Virology ◽

10.1128/jvi.78.20.11360-11370.2004 ◽

2004 ◽

Vol 78 (20) ◽

pp. 11360-11370 ◽

Cited By ~ 174

Author(s):

Jyh-Ming Tsai ◽

Han-Ching Wang ◽

Jiann-Horng Leu ◽

He-Hsuan Hsiao ◽

Andrew H.-J. Wang ◽

...

Keyword(s):

White Spot Syndrome Virus ◽

Expression Patterns ◽

Sodium Dodecyl ◽

Peptide Sequence ◽

White Spot ◽

Structural Proteins ◽

Structural Protein ◽

Structural Genes ◽

Sds Page ◽

White Spot Syndrome

ABSTRACT White spot syndrome virus (WSSV) virions were purified from the hemolymph of experimentally infected crayfish Procambarus clarkii, and their proteins were separated by 8 to 18% gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to give a protein profile. The visible bands were then excised from the gel, and following trypsin digestion of the reduced and alkylated WSSV proteins in the bands, the peptide sequence of each fragment was determined by liquid chromatography-nano-electrospray ionization tandem mass spectrometry (LC-nanoESI-MS/MS) using a quadrupole/time-of-flight mass spectrometer. Comparison of the resulting peptide sequence data against the nonredundant database at the National Center for Biotechnology Information identified 33 WSSV structural genes, 20 of which are reported here for the first time. Since there were six other known WSSV structural proteins that could not be identified from the SDS-PAGE bands, there must therefore be a total of at least 39 (33 + 6) WSSV structural protein genes. Only 61.5% of the WSSV structural genes have a polyadenylation signal, and preliminary analysis by 3′ rapid amplification of cDNA ends suggested that some structural protein genes produced mRNA without a poly(A) tail. Microarray analysis showed that gene expression started at 2, 6, 8, 12, 18, 24, and 36 hpi for 7, 1, 4, 12, 9, 5, and 1 of the genes, respectively. Based on similarities in their time course expression patterns, a clustering algorithm was used to group the WSSV structural genes into four clusters. Genes that putatively had common or similar roles in the viral infection cycle tended to appear in the same cluster.

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A novel envelope protein involved in White spot syndrome virus infection

Journal of General Virology ◽

10.1099/vir.0.80923-0 ◽

2005 ◽

Vol 86 (5) ◽

pp. 1357-1361 ◽

Cited By ~ 28

Author(s):

Ru Huang ◽

Yunli Xie ◽

Jianhong Zhang ◽

Zhengli Shi

Keyword(s):

Escherichia Coli ◽

Virus Infection ◽

Envelope Protein ◽

White Spot Syndrome Virus ◽

Open Reading Frame ◽

White Spot ◽

Structural Protein ◽

Reading Frame ◽

Intact Virion ◽

White Spot Syndrome

One open reading frame (designated vp76) from the White spot syndrome virus (WSSV) genome has the motif of a cytokine I receptor and has been identified as a structural protein. In this paper, vp76 was expressed in Escherichia coli and used to prepare a specific antibody to determine the location of the corresponding protein in the intact virion, the nucleocapsids and the envelope of WSSV. Western blotting with the VP76 antiserum confirmed that VP76 was an envelope protein of WSSV. To investigate the function of the VP76, WSSV was neutralized with the VP76-specific antiserum at different concentrations and injected intramuscularly into crayfish. The mortality curves showed that the VP76 antiserum could partially attenuate infection with WSSV, suggesting that VP76 is an envelope protein involved in WSSV infection.

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Envelope Protein Gene VP466-a Target for PCR Detection of White Spot Syndrome Virus in Shrimp

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v31i1.28468 ◽

2016 ◽

pp. 65-68 ◽

Cited By ~ 1

Author(s):

Anwar Hossain ◽

Santonu Kumar Sanyal ◽

Mohammad Anwar Siddique ◽

Raj Kumar Biswas ◽

Munawar Sultana ◽

...

Keyword(s):

Envelope Protein ◽

White Spot Syndrome Virus ◽

Protein Gene ◽

Polymerase Chain Reaction Method ◽

Pcr Detection ◽

White Spot ◽

Structural Protein ◽

Gene Encoding ◽

White Spot Disease ◽

White Spot Syndrome

White spot syndrome virus (WSSV) is an enveloped and double-stranded DNA virus that belongs to the family Nimaviridae and genus Whispovirus, causing white spot disease (WSD) in shrimp. The virus is highly virulent and leads to 100% mortality within 10 days. Detection of WSSV and segregation of infected brood shrimp, post-larvae and cultured shrimp are currently considered as containment strategies to reduce the spread of WSD. This investigation describes a polymerase chain reaction method to detect WSSV in WSD infected cultured shrimp targeting VP466 gene encoding the large structural protein in virus particle. In silico homology analysis of the primer pair designed in this work clearly identified WSSV VP466 gene sequence with 100% specificity. A total of 16 shrimp samples from 16 farms were selected, where 6 shrimp samples were with characteristics WSD spot and 10 shrimp samples were asymptomatic. Among the 16 shrimp samples, 12 showed PCR positive amplifications for major envelope protein gene VP466. Sequencing of the amplicons followed by homology searching using BLAST further confirmed the presence of WSSV. Phylogenetic analysis of VP466 gene sequences showed its close proximity to the WSSV strain of Indian origin. The present study demonstrates that the envelope protein VP466 gene as a specific target for PCR detection and characterization of WSSV in WSD infected and carrier shrimpsBangladesh J Microbiol, Volume 31, Number 1-2,June-Dec 2014, pp 65-68

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Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

Scientific Reports ◽

10.1038/s41598-021-92002-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Jirayu Boonyakida ◽

Jian Xu ◽

Jun Satoh ◽

Takafumi Nakanishi ◽

Tohru Mekata ◽

...

Keyword(s):

Survival Rate ◽

White Spot Syndrome Virus ◽

Expression System ◽

Penaeid Shrimp ◽

White Spot ◽

Marsupenaeus Japonicus ◽

Structural Protein ◽

E Coli ◽

White Spot Syndrome

AbstractWhite spot syndrome virus (WSSV) is one of the most devastating pathogens in penaeid shrimp and can cause massive damage in shrimp aquaculture industries. Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15(1–25), VP15(26–57), VP15(58–80), and VP15(1–25,58–80). The purified proteins from the E. coli expression system were assayed as potential protective agents in Kuruma shrimp (Marsupenaeus japonicus) using the prime-and-boost strategy. Among the four truncated constructs, VP15(26–57) provided a significant improvement in the shrimp survival rate after 20 days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15(26–57) were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host M. japonicus were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15(26–57), and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.

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Identification of Antigenic Domains and Peptides from VP15 of White Spot Syndrome Virus and their Antiviral Effects in Marsupenaeus Japonicus

10.21203/rs.3.rs-143155/v1 ◽

2021 ◽

Author(s):

Jirayu Boonyakida ◽

Jian Xu ◽

Jun Satoh ◽

Takafumi Nakanishi ◽

Toru Mekata ◽

...

Keyword(s):

Survival Rate ◽

White Spot Syndrome Virus ◽

Expression System ◽

Penaeid Shrimp ◽

White Spot ◽

Marsupenaeus Japonicus ◽

Structural Protein ◽

E Coli ◽

White Spot Syndrome

Abstract White spot syndrome virus (WSSV) is one of the most devastating pathogens in penaeid shrimp and can cause massive damage in shrimp aquaculture industries. Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15(1−25), VP15(26−57), VP15(58−80), and VP15(1−25,58−80). The purified proteins from the E. coli expression system were assayed as potential protective agents in Kuruma shrimp (Marsupenaeus japonicus) using the prime-and-boost strategy. Among the four truncated constructs, VP15(26−57) provided a significant improvement in the shrimp survival rate after 20 days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15(26−57) were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host M. japonicus were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15(26−57), and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.

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