The ontogeny of continuous quantity discrimination in zebrafish larvae (Danio rerio)

Fumonisin B1 (FB1) is a common mycotoxin produced by Fusarium species particularly F. proliferatum and F. verticillioides. The toxin produced can cause adverse effects on humans and animals. The objectives of this study were to detect the production of FB1 based on the amplification of FUM1 gene, to quantify FB1 produced by the isolates using Ultra-fast Liquid Chromatography (UFLC) analysis, to examine the embryotoxicity effect of FB1 and to determine EC50 toward the larvae of zebrafish (Danio rerio). Fifty isolates of Fusarium species were isolated from different hosts throughout Malaysia. Successful amplification of the FUM1 gene showed the presence of this gene (800 bp) in the genome of 48 out of 50 isolates. The highest level of FB1 produced by F. proliferatum isolate B2433 was 6677.32 ppm meanwhile F. verticillioides isolate J1363 was 954.01 ppm. From the assessment of embryotoxicity test of FB1 on larvae of zebrafish, five concentrations of FB1 (0.43 ppm, 0.58 ppm, 0.72 ppm, 0.87 ppm and 1.00 ppm) were tested. Morphological changes of the FB1 exposed-larvae were observed at 24 to 168 hpf. The mortality rate and abnormality of zebrafish larvae were significantly increased at 144 hpf exposure. Meanwhile, the spontaneous tail coiling showed a significant difference. There were no significant differences in the heartbeat rate. As a conclusion, the presence of FUM1 in every isolate can be detected by FUM1 gene analysis and both of the species produced different concentrations of FB1. This is the first report of FB1 produced by Fusarium species gave a significant effect on zebrafish development.

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Protection of Centella asiatica Extract Through BDNF Expression on Stunting Model Zebrafish Larvae (Danio rerio) by Rotenone Induced

Proceedings of the 1st Paris Van Java International Seminar on Health, Economics, Social Science and Humanities (PVJ-ISHESSH 2020) ◽

10.2991/assehr.k.210304.170 ◽

2021 ◽

Author(s):

Annisa Ridlayanti ◽

Aida Ratna Wijayanti ◽

Husnul Khotimah ◽

Muljohadi Ali

Keyword(s):

Danio Rerio ◽

Centella Asiatica ◽

Bdnf Expression ◽

Zebrafish Larvae

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Arginine exposure alters ectonucleotidase activities and morphology of zebrafish larvae ( Danio rerio )

International Journal of Developmental Neuroscience ◽

10.1016/j.ijdevneu.2012.09.002 ◽

2012 ◽

Vol 31 (1) ◽

pp. 75-81 ◽

Cited By ~ 15

Author(s):

Katiucia Marques Capiotti ◽

Lidiane Fazenda ◽

Luiza Reali Nazario ◽

Fabiano Peres Menezes ◽

Luiza Wilges Kist ◽

...

Keyword(s):

Danio Rerio ◽

Zebrafish Larvae

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Quantity discrimination in fish species: fish use non-numerical continuous quantity traits to select shoals

Animal Cognition ◽

10.1007/s10071-018-1214-y ◽

2018 ◽

Vol 21 (6) ◽

pp. 813-820 ◽

Cited By ~ 5

Author(s):

Wei Xiong ◽

Lian-Chun Yi ◽

Zhonghua Tang ◽

Xin Zhao ◽

Shi-Jian Fu

Keyword(s):

Fish Species ◽

Quantity Discrimination ◽

Continuous Quantity

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Recovery Effect of a Rutin-Enriched Fraction Prepared From Forsythia koreana Flowers on Alloxan-Induced Pancreatic Islets in Zebrafish Larvae (Danio rerio)

Natural Product Communications ◽

10.1177/1934578x211043731 ◽

2021 ◽

Vol 16 (10) ◽

pp. 1934578X2110437

Author(s):

Yeong-Geun Lee ◽

Youn Hee Nam ◽

Jung Eun Gwag ◽

Jung-Hwan Ko ◽

Kyeong-Hwa Seo ◽

...

Keyword(s):

Mass Spectrometry ◽

Pancreatic Islets ◽

Danio Rerio ◽

Spectroscopic Data ◽

Recovery Effect ◽

Meoh Extract ◽

Simple Method ◽

Zebrafish Larvae ◽

Forsythia Koreana ◽

First Time

A simple method to prepare a high-content rutin fraction from Forsythia koreana flowers (HRFK) is described. Rutin, isolated for the first time from the flowers, was identified from spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared. As a result of HPLC quantitative analysis, the content of rutin was found to be 80.0 ± 0.02% in HRFK. Our previous study reported that F koreana MeOH extract (FK) significantly recovered alloxan-induced pancreatic islets in zebrafish. However, HRFK as well as rutin exhibited an enhanced anti-diabetic effect compared to FK in our latest experiments. In conclusion, HRFK, as well as rutin and FK, have potential as anti-diabetic agents.

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