Recovery Effect of a Rutin-Enriched Fraction Prepared From Forsythia koreana Flowers on Alloxan-Induced Pancreatic Islets in Zebrafish Larvae (Danio rerio)

2021 ◽  
Vol 16 (10) ◽  
pp. 1934578X2110437
Author(s):  
Yeong-Geun Lee ◽  
Youn Hee Nam ◽  
Jung Eun Gwag ◽  
Jung-Hwan Ko ◽  
Kyeong-Hwa Seo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pancreatic Islets ◽  
Danio Rerio ◽  
Spectroscopic Data ◽  
Recovery Effect ◽  
Meoh Extract ◽  
Simple Method ◽  
Zebrafish Larvae ◽  
Forsythia Koreana ◽  
First Time

A simple method to prepare a high-content rutin fraction from Forsythia koreana flowers (HRFK) is described. Rutin, isolated for the first time from the flowers, was identified from spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared. As a result of HPLC quantitative analysis, the content of rutin was found to be 80.0 ± 0.02% in HRFK. Our previous study reported that F koreana MeOH extract (FK) significantly recovered alloxan-induced pancreatic islets in zebrafish. However, HRFK as well as rutin exhibited an enhanced anti-diabetic effect compared to FK in our latest experiments. In conclusion, HRFK, as well as rutin and FK, have potential as anti-diabetic agents.

scholarly journals Recovery effect of lignans and fermented extracts from Forsythia koreana flowers on pancreatic islets damaged by alloxan in zebrafish (Danio rerio)

2019 ◽  
Vol 62 (1) ◽  
Author(s):  
Yeong-Geun Lee ◽  
Isabel Rodriguez ◽  
Youn Hee Nam ◽  
Jung Eun Gwag ◽  
Sang Ho Woo ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Danio Rerio ◽  
Recovery Effect ◽  
Forsythia Koreana

scholarly journals New Antiplasmodial Diterpenes from Gutierrezia Sarothrae

2016 ◽  
Vol 11 (6) ◽  
pp. 1934578X1601100
Author(s):  
Qingxi Su ◽  
Seema Dalal ◽  
Michael Goetz ◽  
Maria B. Cassera ◽  
David G. L Kingston
Keyword(s):  
Mass Spectrometry ◽  
Plasmodium Falciparum ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
Antiplasmodial Activity ◽  
Drug Resistant ◽  
Meoh Extract ◽  
Bioassay Guided Fractionation ◽  
Gutierrezia Sarothrae ◽  
Related Compounds

Bioassay guided fractionation of the MeOH extract of the plant Gutierrezia sarothrae (Asteraceae) using an assay for antiplasmodial activity against the drug-resistant Dd2 strain of Plasmodium falciparum led to the isolation of the two new diterpenes 3α-angeloyloxy-15-hydroxylabda-7,13-dien-16,15-olid-18-oic acid (1) and 3α-angeloyloxy-15-methoxylabda-7,13-dien-16,15-olid-18-oic acid (2). The structures of 1 and 2 were elucidated by interpretation of ID and 2D NMR spectroscopic data, mass spectrometry, and comparison with the data of related compounds reported in the literature. Compound 1 exhibited moderate antiplasmodial activity with an IC50 values of 10.4 ± 4.3 μM.

On Polymorphism of Dysprosium Trichloride

2015 ◽  
Vol 70 (3) ◽  
pp. 153-157 ◽  
Author(s):  
Irina D. Zakirýanova ◽  
Vladimir A. Khokhlov ◽  
Alexander B. Salyulev ◽  
Iraida V. Korzun
Keyword(s):  
Mass Spectrometry ◽  
Spectroscopic Data ◽  
Polymorphic Transformation ◽  
Room Temperature ◽  
Monoclinic Structure ◽  
Phonon Modes ◽  
Monoclinic Crystal ◽  
Raman Spectroscopic ◽  
Temperature Range ◽  
First Time

AbstractFor the first time, the structure of crystalline DyCl3 over a wide temperature range from room temperature to melting point was studied by Raman spectroscopy. The phonon modes (cm–1) of dysprosium trichloride (monoclinic crystal lattice of AlCl3 type, Z=4, CN = 6) at room temperature are 257 (A1g), 201 (Eg), 112 (Eg), 88 (A1g), and 63 (Eg). The monoclinic structure of the crystalline DyCl3 C2h3 symmetry was found to remain constant over the studied temperature range. No polymorphic transformation in the solid state was detected. Gravimetry, calorimetry, and mass spectrometry have been used in addition to support the conclusions made on the basis of Raman spectroscopic data.

Gas-chromatographic determination of polyol profiles in cerebrospinal fluid.

1978 ◽  
Vol 24 (4) ◽  
pp. 545-548 ◽  
Author(s):  
S L Smith ◽  
M Novotny ◽  
E L Weber
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cerebrospinal Fluid ◽  
Chromatographic Determination ◽  
Gas Chromatography Mass Spectrometry ◽  
Glass Capillary ◽  
Simple Method ◽  
Glass Capillary Columns ◽  
First Time

Abstract We describe a simple method for collectively determining polyols in the cerebrospinal fluid. The method consists of protein removal, sample derivatization, and gas chromatography of the trimethylsilylated polyols, with use of glass capillary columns. Nine major polyol constituents, the structures of which were verified by combined gas chromatography/mass spectrometry, can be simultaneously assayed. The presence of three polyols in the cerebrospinal fluid is reported here for the first time.

scholarly journals A New 1,6-Benzoxazocine-5-one Alkaloid isolated from the Aerial Parts of Peristrophe lanceolaria

2014 ◽  
Vol 9 (10) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Wasana Prapalert ◽  
Dammrong Santiarworn ◽  
Saisunee Liawruangrath ◽  
Boonsom Liawruangrath ◽  
Stephen G. Pyne
Keyword(s):  
Antioxidant Activity ◽  
Spectroscopic Data ◽  
Meoh Extract ◽  
Aerial Parts ◽  
Dpph Method ◽  
First Time

A new 1,6-benzoxazocine-5-one alkaloid has been isolated as its butyl acetal derivative (1) along with peristrophine from the n-BuOH and EtOAc fractions of the crude MeOH extract of the aerial parts of Peristrophe lanceolaria growing in Thailand. The structures of these compounds were elucidated on the basis of their spectroscopic data. These compounds were isolated for the first time from P. lanceolaria. The EtOAc and n-BuOH fractions also possessed significant antioxidant activity with IC50 values of 57 and 50 μg/mL, respectively (DPPH method), whereas 1 had an IC50 value of 23 μg/mL.

Zeolites Fit For A Crown: Probing Host-Guest Interactions with Thermogravimetric Methods

2018 ◽  
Author(s):  
Asel Sartbaeva ◽  
Paul R. Raithby ◽  
Remi Castaing ◽  
Antony Nearchou
Keyword(s):  
Mass Spectrometry ◽  
Thermal Analysis ◽  
Differential Thermal Analysis ◽  
Petrochemical Industry ◽  
Rational Use ◽  
New Methodologies ◽  
First Time ◽  
Kinetics Of

Through a combination of thermogravimetry, mass spectrometry and differential thermal analysis, we demonstrate for the first time that all four zeolites show experimental differences in their host-guest interactions with 18C6. In addition, we have estimated the kinetics of 18C6 decomposition, which is a technique that has not been applied to zeolites previously. Using these findings as a toolkit, a more rational use of OSDAs can be utilised to prepare designer zeolites. Furthermore, the new methodologies presented herein can be applied to current zeolites, such as MFI-type zeolites used in the petrochemical industry.

scholarly journals Analysis of Pembrolizumab in Human Plasma by LC-MS/HRMS. Method Validation and Comparison with Elisa

Biomedicines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 621
Author(s):  
Aurélien Millet ◽  
Nihel Khoudour ◽  
Jérôme Guitton ◽  
Dorothée Lebert ◽  
François Goldwasser ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Plasma ◽  
High Resolution Mass Spectrometry ◽  
Internal Standard ◽  
Therapeutic Drug ◽  
Limit Of Quantification ◽  
Immunoglobulin G4 ◽  
Positive Mode ◽  
Surrogate Peptide ◽  
First Time

Pembrolizumab is a humanized immunoglobulin G4-kappa anti-PD1 antibody used in the treatment of different solid tumors or haematological malignancies. A liquid chromatography coupled with a high resolution mass spectrometry (orbitrap technology) method was fully developed, optimized, and validated for quantitative analysis of pembrolizumab in human plasma. A mass spectrometry assay was used for the first time a full-length stable isotope-labelled pembrolizumab-like (Arginine 13C6-15N4 and Lysine 13C6-15N2) as an internal standard; the sample preparation was based on albumin depletion and trypsin digestion and, finally, one surrogate peptide was quantified in positive mode. The assay showed good linearity over the range of 1–100 μg/mL, a limit of quantification at 1 μg/mL, excellent accuracy from 4.4% to 5.1%, and also a between-day precision below 20% at the limit of quantification. In parallel, an in-house ELISA was developed with a linearity range from 2.5 to 50 µg/mL. Then, results were obtained from 70 plasma samples of cancer patients that were treated with pembrolizumab and quantified with both methods were compared using the Passing-Bablok regression analysis and Bland-Altman plotting. The LC-MS/HRMS method is easy to implement in the laboratory for use in the context of PK/PD studies, clinical trials, or therapeutic drug monitoring.

scholarly journals S-N Curve Characterisation for Composite Materials and Prediction of Remaining Fatigue Life Using Damage Function

2021 ◽  
Vol 5 (3) ◽  
pp. 76
Author(s):  
Ho Sung Kim ◽  
Saijie Huang
Keyword(s):  
Fatigue Life ◽  
Composite Materials ◽  
Loading Rate ◽  
Theoretical Method ◽  
Damage Function ◽  
Simple Method ◽  
Arbitrary Choice ◽  
Number Of Cycles ◽  
First Time ◽  
Remaining Fatigue Life

S-N curve characterisation and prediction of remaining fatigue life are studied using polyethylene terephthalate glycol-modified (PETG). A new simple method for finding a data point at the lowest number of cycles for the Kim and Zhang S-N curve model is proposed to avoid the arbitrary choice of loading rate for tensile testing. It was demonstrated that the arbitrary choice of loading rate may likely lead to an erroneous characterisation for the prediction of the remaining fatigue life. The previously proposed theoretical method for predicting the remaining fatigue life of composite materials involving the damage function was verified at a stress ratio of 0.4 for the first time. Both high to low and low to high loadings were conducted for predicting the remaining fatigue lives and a good agreement between predictions and experimental results was found. Fatigue damage consisting of cracks and whitening is described.

scholarly journals Alterations in lipid profile upon uterine fibroids and its recurrence

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Narine M. Tonoyan ◽  
Vitaliy V. Chagovets ◽  
Natalia L. Starodubtseva ◽  
Alisa O. Tokareva ◽  
Konstantin Chingin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Blood Plasma ◽  
Lipid Profile ◽  
Plasma Levels ◽  
Uterine Fibroids ◽  
Control Group ◽  
Cholesterol Esters ◽  
Plasma Samples ◽  
Diagnostic Models ◽  
First Time

AbstractUterine fibroids (UF) is the most common (about 70% cases) type of gynecological disease, with the recurrence rate varying from 11 to 40%. Because UF has no distinct symptomatology and is often asymptomatic, the specific and sensitive diagnosis of UF as well as the assessment for the probability of UF recurrence pose considerable challenge. The aim of this study was to characterize alterations in the lipid profile of tissues associated with the first-time diagnosed UF and recurrent uterine fibroids (RUF) and to explore the potential of mass spectrometry (MS) lipidomics analysis of blood plasma samples for the sensitive and specific determination of UF and RUF with low invasiveness of analysis. MS analysis of lipid levels in the myometrium tissues, fibroids tissues and blood plasma samples was carried out on 66 patients, including 35 patients with first-time diagnosed UF and 31 patients with RUF. The control group consisted of 15 patients who underwent surgical treatment for the intrauterine septum. Fibroids and myometrium tissue samples were analyzed using direct MS approach. Blood plasma samples were analyzed using high performance liquid chromatography hyphened with mass spectrometry (HPLC/MS). MS data were processed by discriminant analysis with projection into latent structures (OPLS-DA). Significant differences were found between the first-time UF, RUF and control group in the levels of lipids involved in the metabolism of glycerophospholipids, sphingolipids, lipids with an ether bond, triglycerides and fatty acids. Significant differences between the control group and the groups with UF and RUF were found in the blood plasma levels of cholesterol esters, triacylglycerols, (lyso) phosphatidylcholines and sphingomyelins. Significant differences between the UF and RUF groups were found in the blood plasma levels of cholesterol esters, phosphotidylcholines, sphingomyelins and triacylglycerols. Diagnostic models based on the selected differential lipids using logistic regression showed sensitivity and specificity of 88% and 86% for the diagnosis of first-time UF and 95% and 79% for RUF, accordingly. This study confirms the involvement of lipids in the pathogenesis of uterine fibroids. A diagnostically significant panel of differential lipid species has been identified for the diagnosis of UF and RUF by low-invasive blood plasma analysis. The developed diagnostic models demonstrated high potential for clinical use and further research in this direction.

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