Performance of microbial identification by MALDI-TOF MS and susceptibility testing by VITEK 2 from positive blood cultures after minimal incubation on solid media

2017 ◽  
Vol 36 (11) ◽  
pp. 2201-2206 ◽  
Author(s):  
S. L. Mitchell ◽  
K. Alby
Keyword(s):  
Susceptibility Testing ◽  
Blood Cultures ◽  
Maldi Tof Ms ◽  
Microbial Identification ◽  
Maldi Tof ◽  
Solid Media ◽  
Vitek 2 ◽  
Tof Ms

scholarly journals Short Incubation of Positive Blood Cultures on Solid Media for Species Identification by MALDI-TOF MS: Which Agar Is the Fastest?

2021 ◽  
Author(s):  
Neele J. Froböse ◽  
Evgeny A. Idelevich ◽  
Frieder Schaumburg
Keyword(s):  
Mass Spectrometry ◽  
Species Identification ◽  
Susceptibility Testing ◽  
Solid Medium ◽  
Blood Cultures ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Solid Media ◽  
Laboratory Results ◽  
Tof Ms

When blood cultures are flagged as positive, they are incubated on solid media to produce enough biomass of the bacterium for identification and susceptibility testing. Rapid turnaround times for laboratory results could save lives, and we wanted to assess which solid medium is best to shorten the time to species identification using MALDI-TOF mass spectrometry.

scholarly journals Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0205603 ◽  
Author(s):  
Marlène Sauget ◽  
Xavier Bertrand ◽  
Didier Hocquet
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Blood Cultures ◽  
Antibiotic Susceptibility Testing ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Direct Rapid Identification from Positive Blood Cultures by MALDI-TOF MS: Specific Focus on Turnaround Times

2021 ◽  
Author(s):  
Hazan Zengin Canalp ◽  
Banu Bayraktar
Keyword(s):  
Blood Culture ◽  
Rapid Identification ◽  
Blood Cultures ◽  
Pathogen Identification ◽  
Short Term ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Solid Media ◽  
Time Required ◽  
Tof Ms

Using MALDI-TOF MS directly from blood culture bottles reduces the time required for pathogen identification, and the turnaround times for final identification have been compared with overnight incubation from solid media in previous studies. However, identification from a short incubation of agar plates has been increasingly accepted and successfully implemented in routine laboratories, but there is no data comparing direct MALDI-TOF MS with the short-term, incubated agar plates.

scholarly journals Misidentification ofCandida guilliermondiiasC. famataamong Strains Isolated from Blood Cultures by the VITEK 2 System

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Si Hyun Kim ◽  
Jeong Hwan Shin ◽  
Jeong Ha Mok ◽  
Shine Young Kim ◽  
Sae Am Song ◽  
...  
Keyword(s):  
Gene Sequencing ◽  
Candida Guilliermondii ◽  
Blood Cultures ◽  
Rrna Gene ◽  
28S Rrna ◽  
Sequencing Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Vitek 2 ◽  
Tof Ms

Introduction. The aim of this study was to differentiate betweenCandida famataandCandida guilliermondiicorrectly by using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and gene sequencing.Methods. Twenty-eightCandidastrains from blood cultures that had been identified asC. famata(N=25),C. famata/C. guilliermondii(N=2), andC. guilliermondii(N=1) by the VITEK 2 system using the YST ID card were included. We identified these strains by MALDI-TOF MS and gene sequencing using the 28S rRNA andITSgenes and compared the results with those obtained by the VITEK 2 system.Results. All 28 isolates were finally identified asC. guilliermondii.Sequencing analysis of the 28S rRNA gene showed 99.80%–100% similarity withC. guilliermondiifor all 28 strains. TheITSgene sequencing of the strains showed 98.34%–100% homology withC. guilliermondii.By MALDI-TOF, we could correctly identify 21 (75%) of 28C. guilliermondiiisolates.Conclusion. We should suspect misidentification whenC. famatais reported by the VITEK 2 system, and we always should keep in mind the possibility of misidentification of any organism when an uncommon species is reported.

scholarly journals Clinical Impact of MALDI-TOF MS Identification and Rapid Susceptibility Testing on Adequate Antimicrobial Treatment in Sepsis with Positive Blood Cultures

PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0156299 ◽  
Author(s):  
Alexia Verroken ◽  
Lydwine Defourny ◽  
Olivier le Polain de Waroux ◽  
Leïla Belkhir ◽  
Pierre-François Laterre ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antimicrobial Treatment ◽  
Blood Cultures ◽  
Clinical Impact ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Correction: Clinical Impact of MALDI-TOF MS Identification and Rapid Susceptibility Testing on Adequate Antimicrobial Treatment in Sepsis with Positive Blood Cultures

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0160537 ◽  
Author(s):  
Alexia Verroken ◽  
Lydwine Defourny ◽  
Olivier le Polain de Waroux ◽  
Leïla Belkhir ◽  
Pierre-François Laterre ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antimicrobial Treatment ◽  
Blood Cultures ◽  
Clinical Impact ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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