scholarly journals Photobiomodulation invigorating collagen deposition, proliferating cell nuclear antigen and Ki67 expression during dermal wound repair in mice

2020 ◽  
Author(s):  
Vijendra Prabhu ◽  
Bola Sadashiva Satish Rao ◽  
Anuradha Calicut Kini Rao ◽  
Keerthana Prasad ◽  
Krishna Kishore Mahato
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Wound Repair ◽  
Significant Rise ◽  
Nuclear Antigen ◽  
Collagen Deposition ◽  
Tissue Samples ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Proliferating Cell

AbstractThe present investigation focuses on understanding the role of photobiomodulation in enhancing tissue proliferation. Circular excision wounds of diameter 1.5 cm were created on Swiss albino mice and treated immediately with 2 J/cm2 and 10 J/cm2 single exposures of the Helium-Neon laser along with sham-irradiated controls. During different days of healing progression (day 5, day 10, and day 15), the tissue samples upon euthanization of the animals were taken for assessing collagen deposition by Picrosirius red staining and cell proliferation (day 10) by proliferating cell nuclear antigen (PCNA) and Ki67. The positive influence of red light on collagen synthesis was found to be statistically significant on day 10 (P < 0.01) and day 15 (P < 0.05) post-wounding when compared to sham irradiation, as evident from the image analysis of collagen birefringence. Furthermore, a significant rise in PCNA (P < 0.01) and Ki67 (P < 0.05) expression was also recorded in animals exposed to 2 J/cm2 when compared to sham irradiation and (P < 0.01) compared to the 10 J/cm2 treated group as evidenced by the microscopy study. The findings of the current investigation have distinctly exhibited the assenting influence of red laser light on excisional wound healing in Swiss albino mice by augmenting cell proliferation and collagen deposition.

scholarly journals Proliferating cell nuclear antigen (PCNA): a new marker to study human colonic cell proliferation.

Gut ◽  
1994 ◽  
Vol 35 (4) ◽  
pp. 530-535 ◽  
Author(s):  
F J Kubben ◽  
A Peeters-Haesevoets ◽  
L G Engels ◽  
C G Baeten ◽  
B Schutte ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Proliferating Cell

scholarly journals Proliferating Cell Nuclear Antigen as the Cell Cycle Sensor for an HLA-Derived Peptide Blocking T Cell Proliferation

2000 ◽  
Vol 164 (12) ◽  
pp. 6188-6192 ◽  
Author(s):  
Xuefeng Ling ◽  
Salar Kamangar ◽  
Michelle L. Boytim ◽  
Zvi Kelman ◽  
Philip Huie ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
T Cell ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
T Cell Proliferation ◽  
Proliferating Cell

R403 – Epidermal Proliferating Cell Nuclear Antigen in Aging Rats

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P178-P178 ◽  
Author(s):  
Tapan K Bhattacharyya ◽  
Paula Jackson ◽  
J Regan Thomas
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Proliferation Index ◽  
Fischer 344 Rats ◽  
Fischer 344 ◽  
Age Related ◽  
Two Parameters ◽  
Proliferating Cell ◽  
Effect Of Age

Problem To determine if epidermal cell proliferation in colony-raised Fischer 344 rats changes with age and diet. Methods Fischer 344 rats fed ad libitum and calorie-restricted (CR) diets were obtained from the NIA colonies, and young, young adult, and old animals from both groups were used for this study (six in each group). Tissue sections from the dorsal skin (DS) and foot plate (FP) were used for immunohistochemical staining of proliferating cell nuclear antigen (PCNA). The proliferation index (PCNA-I) was computed from counts of stained and total number of keratinocytes. Simultaneous measurements of epidermal thickness were obtained from same sections. Data were analyzed with Excel and SPSS 14.0 software for statistics. Results Two-way analysis of variance (ANOVA) was applied to the data to probe the effect of age, diet, and age-diet interaction. A significant effect of age was noticed in the two parameters i.e., DS PCNA (F 3.96, P .011), FP epidermal width (F 3.37, P .021) and FP PCNA-I (F 9.0, P .000). A significant correlation between DS width and PCNA values was also noted (r 0.5, P .01). Conclusion There is a trend of reduction of PCNA positive cells with increasing age irrespective of thickness of epidermis, and this trend is more apparent in CR rats. Significance This cell proliferation study has implications in relation to CR effect on age-related disease conditions, and biogerontology. Support The study was partially funded by the 2007 Leslie Bernstein grant from AAFPRS foundation.

Sign in / Sign up

Export Citation Format

Share Document