Signals induced by exogenous nitric oxide and their role in controlling brown rot disease caused by Monilinia fructicola in postharvest peach fruit

Single-spore isolates of Monilinia fructicola were collected from commercial orchards in South Carolina and Georgia with prolonged past exposure to demethylation inhibitor (DMI) fungicides and from an orchard with no DMI history (baseline population). Sensitivity to propiconazole was determined using the concentration in agar media required to suppress radial growth of mycelium by 50% (EC50. Mean EC50 values from six South Carolina populations were not different from the baseline population (P < 0.05). Two of five populations from Georgia revealed (significantly higher mean EC50 values compared with the baseline population (P < 0.05). Isolates with high (AP5 and AP6) and low (DL71 and DL72) EC50 values were selected to determine disease incidence on peach fruit after protective or curative applications of propiconazole at 0.15 or 0.3 liter/ha (half and full label rate, respectively). Disease incidence was significantly greater on peaches inoculated with AP5 and AP6 after curative treatment with propiconazole at 0.15 liter/ha (P < 0.05). Following protective or curative treatments at 0.3 liter/ha, disease incidence was significantly greater for AP6 but not for AP5. These results suggest that a shift toward reduced sensitivity has developed in some M. fructicola populations from Georgia, and that isolates with reduced sensitivity to propiconazole are more difficult to control in the field. Field testing of DMI fungicides, captan, QoI fungicides, and fenhexamid in experimental orchards) indicated that the DMI fungicides are still among the most efficacious products for brown rot (control, and that new products containing QoI fungicides may be viable disease control alternatives or rotation partners.

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Difference between chitosan and oligochitosan in growth of Monilinia fructicola and control of brown rot in peach fruit

LWT ◽

10.1016/j.lwt.2011.09.023 ◽

2012 ◽

Vol 46 (1) ◽

pp. 254-259 ◽

Cited By ~ 30

Author(s):

Ling-Yu Yang ◽

Jian-Lei Zhang ◽

Carole L. Bassett ◽

Xiang-Hong Meng

Keyword(s):

Brown Rot ◽

Monilinia Fructicola ◽

Peach Fruit ◽

And Control

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Exogenous nitric oxide induces disease resistance againstMonilinia fructicolathrough activating the phenylpropanoid pathway in peach fruit

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.8146 ◽

2016 ◽

Vol 97 (9) ◽

pp. 3030-3038 ◽

Cited By ~ 34

Author(s):

Guangjin Li ◽

Shuhua Zhu ◽

Wenxue Wu ◽

Chang Zhang ◽

Yong Peng ◽

...

Keyword(s):

Nitric Oxide ◽

Disease Resistance ◽

Phenylpropanoid Pathway ◽

Peach Fruit ◽

Exogenous Nitric Oxide

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Cloning and differential expression of a plum single repeat-MYB, PdMYB3, in compatible and incompatible interactions during fungal infection

Canadian Journal of Plant Science ◽

10.4141/cjps2013-009 ◽

2013 ◽

Vol 93 (4) ◽

pp. 599-605 ◽

Cited By ~ 2

Author(s):

Ashraf El-kereamy ◽

Subramanian Jayasankar

Keyword(s):

Fungal Infection ◽

Differential Expression ◽

Potential Role ◽

Brown Rot ◽

Myb Transcription Factor ◽

Economic Damage ◽

Monilinia Fructicola ◽

Resistant Varieties ◽

Rot Disease ◽

Incompatible Interactions

El-kereamy, A. and Jayasankar, S. 2013. Cloning and differential expression of a plum single repeat-MYB, PdMYB3, in compatible and incompatible interactions during fungal infection. Can. J. Plant Sci. 93: 599–605. Enhancing resistance to pathogen attack through conventional breeding is a major challenge, especially in perennial species. Monilinia fructicola fungal infection causes brown rot disease, resulting in economic damage of stone fruits at flowering, pre- and post-harvest stages. The molecular mechanism of resistance to this disease is still not known. In the present study, we cloned and analyzed the expression of a novel MYB transcription factor from European plums (PdMYB3) induced in response to M. fructicola fungal infection. The identified PdMYB3 is a single repeat-MYB protein that contains a conserved SHAQKYF motif. Monilinia fructicola infection induces the expression of PdMYB3 in fruits of four cultivars within 24 h; however, it is differentially expressed in the susceptible and resistant varieties. By comparing four different cultivars we found that PdMYB3 is induced in much higher levels in the susceptible cultivars than the resistant ones. In addition the PdMYB3 expression is higher in the early stages of fruit development prior to pit hardening, suggesting a potential role for PdMYB3 during this stage. Promoter analysis revealed the presence of some hormone cis-elements suggesting a possible role for PdMYB3 gene in transmitting a signal from the hormonal pathways to downstream components during host-pathogen interactions.

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Comparative analysis of Monilinia fructicola and M. laxa isolates from Brazil: monocyclic components of peach brown rot

Ciência Rural ◽

10.1590/0103-8478cr20160300 ◽

2017 ◽

Vol 47 (6) ◽

Cited By ~ 5

Author(s):

Sthela Siqueira Angeli ◽

Louise Larissa May De Mio ◽

Lilian Amorim

Keyword(s):

Disease Incidence ◽

Beta Function ◽

Brown Rot ◽

Maximum Temperature ◽

Monilinia Fructicola ◽

Peach Fruit ◽

Conidia Germination ◽

Production Area ◽

Important Disease

ABSTRACT: Brown rot is the most important disease of peaches in Brazil. The objective of this study was to compare the brown rot monocyclic components from Monilinia fructicola and M. laxa isolates from Brazil on peaches, due to the detection of M. laxa in the São Paulo production area. Conidia germination and pathogen sporulation were assessed in vitro under a temperature range of 5-35oC and wetness duration of 6-48h. Incubation and latent periods, disease incidence, disease severity and pathogen reproduction on peach fruit were evaluated under 10, 15, 20, 25 and 30oC and wetness duration of 6, 12 and 24h. Six of seven parameters of a generalised beta function fitted to conidia germination of M. fructicola and M. laxa were similar. Only the shape parameter was higher for M. fructicola indicating that the range of temperatures and wetness periods favourable for germination is wider for M. laxa than for M. fructicola. The optimum temperature for brown rot development caused by M. fructicola was 24.5oC and for. Monilinia laxa was 19.8oC. At 10oC M. laxa lesions produced more conidia than M. fructicola, and the opposite occurred at 30oC. The estimated maximum temperature for lesion development was also higher for M. fructicola than for M. laxa. M. fructicola is favored by warmer weather than M. laxa and the presence and impact of this specie in Brazil must be investigated especially in the South states.

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