Self- and nonself recognition during hyphal interactions in Rosellinia necatrix

2015 ◽  
Vol 81 (6) ◽  
pp. 420-428 ◽  
Author(s):  
Takahiro Uwamori ◽  
Kanako Inoue ◽  
Chiaki Kida ◽  
Yuichi Morita ◽  
Pyoyun Park ◽  
...  
Keyword(s):  
Rosellinia Necatrix ◽  
Nonself Recognition

scholarly journals A Novel Bipartite Double-Stranded RNA Mycovirus from the White Root Rot Fungus Rosellinia necatrix: Molecular and Biological Characterization, Taxonomic Considerations, and Potential for Biological Control

2009 ◽  
Vol 83 (24) ◽  
pp. 12801-12812 ◽  
Author(s):  
Sotaro Chiba ◽  
Lakha Salaipeth ◽  
Yu-Hsin Lin ◽  
Atsuko Sasaki ◽  
Satoko Kanematsu ◽  
...  
Keyword(s):  
Biological Control ◽  
Root Rot ◽  
Sequence Similarity ◽  
Peptide Mass Fingerprinting ◽  
Dsrna Virus ◽  
Rosellinia Necatrix ◽  
Virus Family ◽  
White Root Rot ◽  
Double Stranded Rna ◽  
Peptide Mass

ABSTRACT White root rot, caused by the ascomycete Rosellinia necatrix, is a devastating disease worldwide, particularly in fruit trees in Japan. Here we report on the biological and molecular properties of a novel bipartite double-stranded RNA (dsRNA) virus encompassing dsRNA-1 (8,931 bp) and dsRNA-2 (7,180 bp), which was isolated from a field strain of R. necatrix, W779. Besides the strictly conserved 5′ (24 nt) and 3′ (8 nt) terminal sequences, both segments show high levels of sequence similarity in the long 5′ untranslated region of approximately 1.6 kbp. dsRNA-1 and -2 each possess two open reading frames (ORFs) named ORF1 to -4. Although the protein encoded by 3′-proximal ORF2 on dsRNA-1 shows sequence identities of 22 to 32% with RNA-dependent RNA polymerases from members of the families Totiviridae and Chrysoviridae, the remaining three virus-encoded proteins lack sequence similarities with any reported mycovirus proteins. Phylogenetic analysis showed that the W779 virus belongs to a separate clade distinct from those of other known mycoviruses. Purified virions ∼50 nm in diameter consisted of dsRNA-1 and -2 and a single major capsid protein of 135 kDa, which was shown by peptide mass fingerprinting to be encoded by dsRNA-1 ORF1. We developed a transfection protocol using purified virions to show that the virus was responsible for reduction of virulence and mycelial growth in several host strains. These combined results indicate that the W779 virus is a novel bipartite dsRNA virus with potential for biological control (virocontrol), named Rosellinia necatrix megabirnavirus 1 (RnMBV1), that possibly belongs to a new virus family.

scholarly journals Draft Genome Sequence and Transcriptional Analysis of Rosellinia necatrix Infected with a Virulent Mycovirus

2018 ◽  
Vol 108 (10) ◽  
pp. 1206-1211 ◽  
Author(s):  
Takeo Shimizu ◽  
Satoko Kanematsu ◽  
Hajime Yaegashi
Keyword(s):  
Genome Sequence ◽  
Molecular Mechanisms ◽  
Plant Cell Wall ◽  
Draft Genome ◽  
Transcriptional Analysis ◽  
Draft Genome Sequence ◽  
Effective Control ◽  
Economic Losses ◽  
Rosellinia Necatrix ◽  
Cell Wall Degrading Enzymes

Understanding the molecular mechanisms of pathogenesis is useful in developing effective control methods for fungal diseases. The white root rot fungus Rosellinia necatrix is a soilborne pathogen that causes serious economic losses in various crops, including fruit trees, worldwide. Here, using next-generation sequencing techniques, we first produced a 44-Mb draft genome sequence of R. necatrix strain W97, an isolate from Japan, in which 12,444 protein-coding genes were predicted. To survey differentially expressed genes (DEGs) associated with the pathogenesis of the fungus, the hypovirulent W97 strain infected with Rosellinia necatrix megabirnavirus 1 (RnMBV1) was used for a comprehensive transcriptome analysis. In total, 545 and 615 genes are up- and down-regulated, respectively, in R. necatrix infected with RnMBV1. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses of the DEGs suggested that primary and secondary metabolism would be greatly disturbed in R. necatrix infected with RnMBV1. The genes encoding transcriptional regulators, plant cell wall-degrading enzymes, and toxin production, such as cytochalasin E, were also found in the DEGs. The genetic resources provided in this study will accelerate the discovery of genes associated with pathogenesis and other biological characteristics of R. necatrix, thus contributing to disease control.

Rosellinia necatrix. [Distribution map].

1976 ◽  
Author(s):  
Keyword(s):  
South Africa ◽  
New York ◽  
Central America ◽  
West Indies ◽  
Central African Republic ◽  
Rio Grande ◽  
Republic Of Georgia ◽  
Rosellinia Necatrix ◽  
Distribution Map ◽  
New Distribution

Abstract A new distribution map is provided for Rosellinia necatrix Prill. Hosts: Various. Information is given on the geographical distribution in AFRICA, Central African Republic, Ethiopia, Kenya, Morocco, Mozambique, South Africa, Zaire, ASIA, China, India (Himachal Pradesh), Iran, Iraq, Israel, Japan, Korea, Lebanon, Philippines, Turkey, USSR (Caucasus, Crimea) (Republic of Georgia), Middle, Asia, AUSTRALASIA & OCEANIA, New Zealand, EUROPE, Austria, Britain, Bulgaria, Cyprus, France, Germany, Greece, Italy, Portugal (Azores), Romania, Spain, Switzerland, USSR (S.), Yugoslavia, NORTH AMERICA, Mexico, USA (Alabama, California, Indiana, Michigan, New York, Ohio), CENTRAL AMERICA & WEST INDIES, Dominican Republic, SOUTH AMERICA, Argentina, Brazil (Rio Grande, Sao Paulo), Colombia, Uruguay.

Efficacy of Potential Control Agents Against Rosellinia necatrix and Their Physiological Impact on Avocado

Plant Disease ◽  
2021 ◽  
Author(s):  
Phinda Magagula ◽  
Nicky Taylor ◽  
Velushka Swart ◽  
Noëlani van den Berg
Keyword(s):  
Integrated Management ◽  
Integrated Approach ◽  
Dry Mass ◽  
Persea Americana ◽  
Effective Control ◽  
Rosellinia Necatrix ◽  
Potential Control ◽  
Greenhouse Trial

Rosellinia necatrix is the causal agent of white root rot (WRR), a fatal disease affecting many woody plants, including avocado (Persea americana). As with other root diseases, an integrated approach is required to control WRR. No fully effective control methods are available, and no chemical or biological agents against R. necatrix have been registered for use on avocado in South Africa. Fluazinam has shown promising results in the greenhouse and field in other countries, including Spain. The current study aimed to investigate the potential of a fumigant, chloropicrin, and biological control agents (B-Rus, Beta-Bak, Mity-Gro, and Trichoderma) against R. necatrix both in vitro and in vivo as compared with fluazinam. In a greenhouse trial, results showed that Trichoderma and B-Rus were as effective as fluazinam at inhibiting R. necatrix in vitro and suppressed WRR symptoms when applied before inoculation with R. necatrix. In contrast, Mity-Gro and Beta-Bak failed to inhibit the pathogen in vitro and in the greenhouse trial, despite application of the products to plants before R. necatrix infection. Fluazinam suppressed WRR symptoms in plants when applied at the early stages of infection, whereas chloropicrin rendered the pathogen nonviable when used as a preplant treatment. Plants treated with Trichoderma, B-Rus, and fluazinam sustained dry mass production and net CO2 assimilation by maintaining the green leaf tissues despite being infected with the pathogen. This study has important implications for the integrated management of WRR.

Rosellinia necatrix. [Distribution map].

1987 ◽  
Author(s):  
Keyword(s):  
South Africa ◽  
New York ◽  
Central America ◽  
West Indies ◽  
Central African Republic ◽  
Rio Grande ◽  
Rosellinia Necatrix ◽  
Rio Grande Do Sul ◽  
Distribution Map ◽  
New Distribution

Abstract A new distribution map is provided for Rosellinia necatrix Prill. Hosts: Various. Information is given on the geographical distribution in Africa, Central African Republic, Ethiopia, Kenya, Morocco, Mozambique, South Africa, Zaire, Asia, China, India, Himachal Pradesh, Iran, Iraq, Israel, Japan, Korea, Lebanon, Philippines, Taiwan, Turkey, USSR, Caucasus, Crimea, Georgia, Middle Asia, Australasia & Oceania, New Zealand, Europe, Austria, Britain, Bulgaria, Cyprus, France, Germany, Greece, Italy, Portugal, Azores, Romania, Spain, Switzerland, Yugoslavia, North America, Mexico, USA, Alabama, California, Indiana, Michigan, New York, Ohio, Central America & West Indies, Dominican Republic, South America, Argentina, Brazil, Rio Grande do Sul, Sao Paulo, Colombia, Uruguay.

scholarly journals Weed Roots Facilitate the Spread of Rosellinia necatrix, the Causal Agent of White Root Rot

2019 ◽  
Vol 34 (3) ◽  
pp. 340-343
Author(s):  
Hayato Shiragane ◽  
Toshiyuki Usami ◽  
Masahiro Shishido
Keyword(s):  
Root Rot ◽  
Causal Agent ◽  
Rosellinia Necatrix ◽  
White Root Rot
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