Effects of hydroxycinnamic acid esters on sweetpotato weevil feeding and oviposition and interactions with Bacillus thuringiensis proteins

AbstractSweetpotato weevil (SPW) pest management is challenging because the pest target is sub-terranean, so the application of pesticides is impractical and usually ineffective. Host plant resistance and the genetic transformation of sweetpotatoes to produce entomotoxic Bt proteins offer potential for environmentally benign pest control. Resistance can be conferred by naturally occurring hydroxycinnamic acids which protect against oviposition by adults, but these compounds are restricted to the root surface so do not protect against the cortex bound larvae where the greatest damage occurs. Resistance could be enhanced if combined with expression of Bt proteins in transformed plants, but interactions between hydroxycinnamic acids and Bt proteins remain unknown. Here the bioactivity of Cry7Aa1 protein and hydroxycinnamic acid esters was evaluated individually and in combination against SPW larvae and mortality determined. Low and high concentrations of hydroxycinnamic acid esters alone caused significantly higher mortality of both weevil species in all experiments compared to the control. SPW larval mortality was greater when tested as a combination of hydroxycinnamic acid esters and Bt protein, but this effect was additive not synergistic. Although we report no evidence of antagonistic interactions, the antifeedant effects of the plant compounds conferring host plant resistance could have reduced consumption of the Bt protein in our assays leading to a lower efficacy when combined. Further work is required to determine whether the toxic effects of Bt proteins function alongside host plant resistance in sweetpotato under field conditions.

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Sweetpotato Volatile Chemistry in Relation to Sweetpotato Weevil (Cylas formicarius) Behavior

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.127.4.656 ◽

2002 ◽

Vol 127 (4) ◽

pp. 656-662 ◽

Cited By ~ 17

Author(s):

Yan Wang ◽

Stanley J. Kays

Keyword(s):

Gas Chromatography ◽

Host Plant ◽

Plant Resistance ◽

Host Plant Resistance ◽

Biologically Active ◽

Volatile Fraction ◽

Storage Roots ◽

Sweetpotato Weevil ◽

Plant Parts ◽

Cylas Formicarius

The sweetpotato weevil (SPW) [Cylas formicarius elegantulus (Summers) (Coleoptera: Curculionidae)] is the single most devastating pest of the sweetpotato [Ipomoea batatas (L.) Lam.] worldwide. Attempts to develop host-plant resistance have been only moderately successful due in part to deficiencies in parent and progeny selection methods. Host-plant phytochemicals play critical roles in insect behavior, modulating a cross-section of key behavioral decisions. Thus, identification of the phytochemicals the female weevil uses in decision making could greatly facilitate development of host-plant resistance. The volatile chemistry of the sweetpotato was studied in relation to the host-finding behavior of the female weevil. Critical biologically active volatiles were determined via isolation (Tenax trapping), fractionation (gas chromatography-thermal conductivity detector), identification (gas chromatography and gas chromatography-mass spectroscopy), and bioassay (olfactometry). Differences in volatile chemistry among sweetpotato clones that may relate to differences in resistance or susceptibility to the female SPW were assessed. Volatile extracts from storage roots (site of oviposition) and aerial plant parts were attractive to female SPW, the former being substantially greater. In total, 33 compounds were identified from storage roots and aerial plant parts, including 23 terpenes. Three oxygenated monoterpenes (nerol, Z-citral, and methyl geranate), found in storage roots but not aerial plant parts, were identified as attractants. The sesquiterpene volatile fraction was repellent to female SPW with α-gurjunene, α-humulene, and ylangene active in the concentration range emanating from storage roots. The aerial plant parts emanated a higher composite concentration of sesquiterpenes than storage roots. Differences in the relative attraction among four sweetpotato cultivars to female SPW was inversely correlated with the composite concentration of headspace sesquiterpenes. Selection of clones with decreased volatile attractants and/or increased deterrents using an analytical means of quantification may significantly facilitate developing resistance to the SPW.

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Segregation of Hydroxycinnamic Acid Esters Mediating Sweetpotato Weevil Resistance in Storage Roots of Sweetpotato

Frontiers in Plant Science ◽

10.3389/fpls.2017.01011 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 3

Author(s):

Milton O. Anyanga ◽

Benard Yada ◽

G. C. Yencho ◽

Gorrettie N. Ssemakula ◽

Agnes Alajo ◽

...

Keyword(s):

Hydroxycinnamic Acid ◽

Storage Roots ◽

Sweetpotato Weevil ◽

Hydroxycinnamic Acid Esters ◽

Acid Esters

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Synthesis of hydroxycinnamic acid esters of betacyanins via 1-O-acylglucosides of hydroxycinnamic acids by protein preparations from cell suspension cultures of Chenopodium rubrum and petals of Lampranthus sociorum

Planta ◽

10.1007/bf00394880 ◽

1988 ◽

Vol 174 (1) ◽

pp. 101-105 ◽

Cited By ~ 31

Author(s):

Maria Bokern ◽

Dieter Strack

Keyword(s):

Cell Suspension ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Hydroxycinnamic Acid ◽

Hydroxycinnamic Acids ◽

Chenopodium Rubrum ◽

Hydroxycinnamic Acid Esters ◽

Acid Esters

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Host plant resistance and biological control: A test of the movement-risk hypothesis

10.1603/ice.2016.109191 ◽

2016 ◽

Author(s):

Cory Straub

Keyword(s):

Biological Control ◽

Host Plant ◽

Plant Resistance ◽

Host Plant Resistance

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Morphological and Chemical Factors Related to Western Flower Thrips Resistance in the Ornamental Gladiolus

Plants ◽

10.3390/plants10071384 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1384

Author(s):

Dinar S. C. Wahyuni ◽

Young Hae Choi ◽

Kirsten A. Leiss ◽

Peter G. L. Klinkhamer

Keyword(s):

Host Plant ◽

Plant Resistance ◽

Host Plant Resistance ◽

Frankliniella Occidentalis ◽

Western Flower Thrips ◽

Resistance Breeding ◽

Breeding Programs ◽

Triterpenoid Saponins ◽

Flower Thrips ◽

Highly Correlated

Understanding the mechanisms involved in host plant resistance opens the way for improved resistance breeding programs by using the traits involved as markers. Pest management is a major problem in cultivation of ornamentals. Gladiolus (Gladiolus hybridus L.) is an economically important ornamental in the Netherlands. Gladiolus is especially sensitive to attack by western flower thrips (Frankliniella occidentalis (Pergande) (Thysanoptera:Thripidae)). The objective of this study was, therefore, to investigate morphological and chemical markers for resistance breeding to western flower thrips in Gladiolus varieties. We measured thrips damage of 14 Gladiolus varieties in a whole-plant thrips bioassay and related this to morphological traits with a focus on papillae density. Moreover, we studied chemical host plant resistance to using an eco-metabolomic approach comparing the 1H NMR profiles of thrips resistant and susceptible varieties representing a broad range of papillae densities. Thrips damage varied strongly among varieties: the most susceptible variety showed 130 times more damage than the most resistant one. Varieties with low thrips damage had shorter mesophylls and epidermal cells, as well as a higher density of epicuticular papillae. All three traits related to thrips damage were highly correlated with each other. We observed a number of metabolites related to resistance against thrips: two unidentified triterpenoid saponins and the amino acids alanine and threonine. All these compounds were highly correlated amongst each other as well as to the density of papillae. These correlations suggest that papillae are involved in resistance to thrips by producing and/or storing compounds causing thrips resistance. Although it is not possible to distinguish the individual effects of morphological and chemical traits statistically, our results show that papillae density is an easy marker in Gladiolus-breeding programs targeted at increased resistance to thrips.

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Comparative TMT Proteomic Analysis Unveils Unique Insights into Helicoverpa armigera (Hübner) Resistance in Cajanus scarabaeoides (L.) Thouars

International Journal of Molecular Sciences ◽

10.3390/ijms22115941 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5941

Author(s):

Abigail Ngugi-Dawit ◽

Isaac Njaci ◽

Thomas J.V. Higgins ◽

Brett Williams ◽

Sita R. Ghimire ◽

...

Keyword(s):

Host Plant ◽

Helicoverpa Armigera ◽

Plant Resistance ◽

Host Plant Resistance ◽

Proteomic Analysis ◽

Artificial Diet ◽

Growth Stages ◽

Genetic Base ◽

Helicoverpa Armígera ◽

Helicoverpa Armigera Hubner

Pigeonpea [Cajanus cajan (L.) Millspaugh] is an economically important legume playing a crucial role in the semi-arid tropics. Pigeonpea is susceptible to Helicoverpa armigera (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to H. armigera, respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. H. armigera tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, antioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3’-diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.

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