Chemical composition and acaricidal activity of Eucalyptus globulus essential oil against the vector of tropical bovine piroplasmosis, Rhipicephalus (Boophilus) annulatus

Abstract The study was aimed at determining the chemical composition, toxicity effect and field varroacidal efficacy of the essential oil distilled from the leaf of Eucalyptus globulus (Eg) grown in Algeria. Brine shrimp lethality (BSL) assay and bee hives infected by Varroa destructor were used to assess the toxicity and acaricidal effect, respectively. Steam distillation of leaves yielded 0.93 % (v/w) of essential oil (EO). GC/MS Analysis revealed 39 compounds, essentially oxygenated monoterpenes (86.01%). The main constituents of the oil were 1,8-cineole (78.45 %), o-cymene (2.18 %), isopinocarveole (1.74 %), α-pinene (1.69 %), pinocarvone (1.34%) and veridiflorol (1.31%). The BSL assay revealed a highly toxic value of LC50 (67.55 μg/mL). Furthermore, field efficacy testing on bee hives infected with Varroa destructor has confirmed the effectiveness of Eucalyptus globulus essential oil (EgEO) or thymol as varroacidal agents. Moreover, a EgEO + thymol association was more effective than EgEO or thymol alone. Finally, the use of EgEO + thymol may constitute a viable alternative to the thymol-based commercial treatment

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Chemical composition and insecticidal activity of Garcinia gardneriana (Planchon & Triana) Zappi (Clusiaceae) essential oil

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas ◽

10.37360/blacpma.21.20.5.37 ◽

2021 ◽

Vol 20 (5) ◽

pp. 503-514

Author(s):

Carla Maria Mariano Fernandez ◽

◽

Fabiana Brusco Lorenzetti ◽

Sirlene Adriana Kleinubing ◽

Joao Paulo Pinguello de Andrade ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

Chemical Analysis ◽

Essential Oil ◽

Immersion Test ◽

Acaricidal Activity ◽

Gas Chromatography Mass Spectrometry ◽

Larvicidal Effect ◽

Sesquiterpene Hydrocarbons

The present study aimed to analyze the chemical composition of the essential oil from Garcinia gardneriana (Planchon & Triana) Zappi leaves and fruits, and to determine its acaricidal activity on Rhipicephalus microplus by larval packet test and larvicidal action on Aedes aegypti by larval immersion test. The chemical analysis of the essential oil by gas chromatography-mass spectrometry identified sesquiterpene hydrocarbons and oxygenated sesquiterpenes in bacupari leaves and fruits, and α-cedrene, α-chamigrene, α-trans-bergamotene, and β-curcumene as major compounds. Essential oil from leaves of G. gardneriana presented acaricidal activity on R. microplus (LC50 = 4.8 mg/mL; LC99 = 10.8 mg/mL) and larvicidal effect on A. aegypti (LC50 = 5.4 mg/mL; LC99 = 11.6 mg/mL), whereas essential oil from the fruits of G. gardneriana showed LC50 = 4.6 mg/mL and LC99 = 8.9 mg/mL against R. microplus and LC50 = 6.4 mg/mL and LC99 = 13.9 mg/mL against A. aegypti. These results thus demonstrate the potential acaricidal and larvicidal activity of essential oil of G. gardneriana, offering new perspectives for the realization of bioassays from this essential oil.

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Chemical Composition and Acaricidal Activity of Nemuaron vieillardii Essential Oil against the Cattle Tick Rhipicephalus (Boophilus) microplus

Natural Product Communications ◽

10.1177/1934578x1601101235 ◽

2016 ◽

Vol 11 (12) ◽

pp. 1934578X1601101

Author(s):

Nicolas Lebouvier ◽

Thomas Hue ◽

Joseph Brophy ◽

Edouard Hnawia ◽

Mohammed Nour

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Tick Control ◽

Acaricidal Activity ◽

Boophilus Microplus ◽

Gas Chromatography Mass Spectrometry ◽

Cattle Tick ◽

Caryophyllene Oxide ◽

Human Carcinogen

Essential oil from leaves of Nemuaron vieillardii (Baill.) Baill., a shrub used in the kanak pharmacopeia, was analyzed by gas chromatography and combined gas chromatography-mass spectrometry. The main compounds identified were safrole (49.7%), linalool (8.0%), δ-cadinene (5.1%), caryophyllene oxide (4%) and α-copaene (2.4%). This chemical composition is consistent with leaf oils of the Atherospermataceae family and demonstrates the proximity of the two genera Nemuaron and Atherosperma as regards to their essential oil compositions. The modified Larval Packet Test (LPT) was used to assess acaricidal effect of N. vieillardii essential oil on larvae of the cattle tick Rhipicephalus (Boophilus) microplus and LC50 was then calculated to 14.67%. High content of safrole in this oil which is suspected of being a human carcinogen, poses the problem of the use of this oil in a perspective of development of alternative tick control strategy and in the traditional medicinal consumption of Nemuaron vieillardii.

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Chemical composition and phytotoxic potential of Eucalyptus globulus essential oil against Lactuca sativa and two herbicide-resistant weeds: Avena fatua and Amaranthus hybridus

TIP Revista Especializada en Ciencias Químico-Biológicas ◽

10.22201/fesz.23958723e.2021.303 ◽

2021 ◽

Vol 24 ◽

Author(s):

Antonio Flores-Macías ◽

Guadalupe Georgina Reyes-Zarate ◽

Claudio Augusto Gomes da Camara ◽

Reyes Reyes-López ◽

Juan Campos Guillén ◽

...

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Lactuca Sativa ◽

Eucalyptus Globulus ◽

Avena Fatua ◽

Radicle Growth ◽

Amaranthus Hybridus ◽

Flame Ionization ◽

Gas Chromatography Mass Spectroscopy

Weed control in sustainable agriculture requires new bioherbicidal molecules to replace synthetic herbicides that have damaged the environment and generated resistance in weeds. This study was conducted to investigate the chemical composition of Eucalyptus globulus essential oil and to explore its bioherbicidal potential on the germination and radicle growth inhibition. The phytotoxic effects of E. globulus essential oil (1, 10 and 20 μL mL-1) were tested in comparison to those of the synthetic herbicide Glyphosate (1, 10 and 20 μL mL-1) in bioassays of germination and radicle growth of Lactuca sativa and the resistant weeds Avena fatua and Amaranthus hybridus. Gas Chromatography with Flame Ionization Detector and Gas Chromatography-Mass Spectroscopy analysis showed that major monoterpenes comprised 1,8-Cineole (86.94%), α-pinene (7.71%), d-limonene (2.65%), and p-cymene (1.48%). The seed germination and radicle length exhibited different degrees of inhibition in response to the concentration of E. globulus essential oil. At some concentrations, both the Glyphosate herbicide and the E. globulus essential oil demonstrated the same phytotoxicity against the resistant weeds A. fatua and A. hybridus. Essential oil bioactivity Lethal Concentration (LC50) in the majority of cases was lowest for A. hybridus, followed by A. fatua and L. sativa. Based on the results, it can be concluded that E. globulus essential oil possesses phytotoxic potential and could be explored as a bioherbicide for resistant weeds management programs.

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