Molecular identification and morphological variations of Dermacentor albipictus collected from two deer species in northern Mexico

Author(s):  
Sharai Montiel-Armendáriz ◽  
Claudio Verdugo ◽  
Alan Esteban Juache-Villagrana ◽  
Florinda Jiménez-Vega ◽  
Andrés Quezada-Casasola ◽  
...  
2018 ◽  
Vol 19 (2) ◽  
pp. 364-368
Author(s):  
LULUT DWI SULISTYANINGSIH ◽  
MARLINA ARDIYANI ◽  
ABINAWANTO ABINAWANTO ◽  
ANDI SALAMAH

Sulistyaningsih LD, Abinawanto, Ardiyani M, Salamah A. 2018. Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis. Biodiversitas 19: 364-368. Smilax spp. (Smilacaceae) has long been used as medicinal herbs especially in East Asia and North America as they were known to be rich in steroidal saponin. Pharmacological study has been carried out in Indonesia. This genus is widespread in Indonesia and fairly abundant in Java and has been known either as edible fruit or medicinal plants. Characteristics of Smilax as a dioecious plant with high morphological variations make it thorny in species identification. Various molecular approaches have been devised to overcome identification problems such as DNA barcoding. This study, therefore was conducted to analyze the DNA barcoding application for phylogenetic and identification of Smilax in Java. A total of 31 samples were used in this study including 19 accession numbers from NCBI GeneBank. The genus Ripogonum was used as the out-group in phylogenetic reconstruction. Samples were successfully extracted by CTAB method with some modifications. rbcL region was used as the DNA barcode showed sufficient variation and conserved flanks. Two unidentified specimens have high similarity with S. leucophyla and lies in the same clade. The phylogenetic tree constructed by Maximum Likelihood analysis. The result showed that the monophyletic of Smilacaceae consisted of four clades. The genus Heterosmilax nested with Smilax though with low bootstraps value. It supports the monogeneric status of Smilacaceae.


2007 ◽  
Vol 43 (3) ◽  
pp. 504-507 ◽  
Author(s):  
Antonio Cantu ◽  
J. Alfonso Ortega-S ◽  
Juan Mosqueda ◽  
Zeferino Garcia-Vazquez ◽  
Scott E. Henke ◽  
...  

Author(s):  
S. E. Miller

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.


Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
CA López-Moreno ◽  
LR Quintanilla ◽  
GLB Serrano ◽  
QE Rosales ◽  
FJA Pérez ◽  
...  

2018 ◽  
Vol 592 ◽  
pp. 225-242 ◽  
Author(s):  
E Cruz-Acevedo ◽  
N Tolimieri ◽  
H Aguirre-Villaseñor

2015 ◽  
Vol 31 (2) ◽  
pp. 287-304
Author(s):  
Raphael B. Folsom

The writings of the U.S. scholar Philip Wayne Powell have had an enduring influence on the historiography of colonial Mexico and the Spanish borderlands. But his writings have never been examined as a unified corpus, and so the deeply reactionary political ideology that lay behind them has never been well understood. By analyzing Powell’s political convictions, this article shows how contemporary scholarship on the conquest of northern Mexico can emerge from Powell’s long shadow. Los escritos del estudioso estadounidense Philip Wayne Powell han ejercido una influencia perdurable sobre la historiografía del México colonial y las zonas fronterizas españolas. Sin embargo, dichos escritos nunca han sido examinados como un corpus unificado, de manera que la ideología política profundamente reaccionaria detrás de ellos nunca ha sido bien comprendida. Al analizar las convicciones políticas de Powell, el presente artículo muestra cómo puede surgir un conocimiento contemporáneo sobre la conquista del norte de México a partir de la larga sombra de Powell.


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