Evaluation of Trichoderma harzianum strain T22 as biological control agent of Calonectria pauciramosa

BioControl ◽  
2011 ◽  
Vol 57 (5) ◽  
pp. 687-696 ◽  
Author(s):  
Alessandro Vitale ◽  
Gabriella Cirvilleri ◽  
Ivana Castello ◽  
Dalia Aiello ◽  
Giancarlo Polizzi
Keyword(s):  
Biological Control ◽  
Trichoderma Harzianum ◽  
Biological Control Agent ◽  
Control Agent

scholarly journals EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG

2017 ◽  
Vol 16 (2) ◽  
pp. 115
Author(s):  
Johanna Taribuka ◽  
Christanti Sumardiyono ◽  
Siti Muslimah Widyastuti ◽  
Arif Wibowo
Keyword(s):  
Biological Control ◽  
Phylogenetic Analysis ◽  
Endophytic Fungi ◽  
Trichoderma Harzianum ◽  
Biocontrol Agent ◽  
Biological Control Agent ◽  
Control Agent ◽  
Selective Medium ◽  
Trichoderma Spp ◽  
Its1 And Its2

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.

scholarly journals Pellet Formulation of Trichoderma Isolated from Aceh with Potential for Biocontrol of Phytophthora Sp. on Cacao Sedlings

2019 ◽  
Vol 3 (2) ◽  
pp. 202-212
Author(s):  
Rina Sriwati ◽  
Tjut Chamzhurni ◽  
Alfizar Alfizar ◽  
Bonny PW Soekarno ◽  
Vina Maulidia ◽  
...  
Keyword(s):  
Biological Control ◽  
Disease Severity ◽  
Trichoderma Harzianum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Diameter Growth ◽  
Good Ability ◽  
Trichoderma Isolate ◽  
Pellet Formulation

Molleculler study was conducted to identify several species of Trichoderma isolate from several  plant (Pine, Cacao, Gliceria, Nutmeg, Bamboo, Coffee, Potato).  The growth of eight species Trichodermaafter pelleting formulation has been observed. Pellet Trichoderma harzianum have good ability to growth on PDA medium after 4 weeks storage. Base on their mycelium diameter growth on PDA, T. harzianum have selected as potential species on pellet formulation growth.  Several dose of pellet formulation have been applied for controlling Phythopthora disease. The application of T. harzianum pellets in the form of a 2 g / 100 ml (S1) suspension effective in inhibiting the development of Phytophthora sp in cacao seedlings, when the higher concentrations of T. harzianum pellets applied to cacao seeds,the disease severity increase. Pellet Trichoderma could be use as biological control agent of cacao seedling in certain dosage.

qRT-PCR quantification of the biological control agent Trichoderma harzianum in peat and compost-based growing media

2011 ◽  
Vol 102 (3) ◽  
pp. 2793-2798 ◽  
Author(s):  
Robert Beaulieu ◽  
Rubén López-Mondéjar ◽  
Fabio Tittarelli ◽  
Margarita Ros ◽  
José Antonio Pascual
Keyword(s):  
Biological Control ◽  
Trichoderma Harzianum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Growing Media ◽  
Qrt Pcr

scholarly journals Generation of Trichoderma harzianum with pyr4 auxotrophic marker by using the CRISPR/Cas9 system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amanda A. Vieira ◽  
Giovanni R. Vianna ◽  
Jessica Carrijo ◽  
Francisco J. L. Aragão ◽  
Pabline M. Vieira
Keyword(s):  
Biological Control ◽  
Trichoderma Harzianum ◽  
Biological Control Agent ◽  
Control Agent ◽  
Phenotypic Characterization ◽  
Agricultural Pests ◽  
Control Activity ◽  
Guide Rna ◽  
Endogenous Expression ◽  
Auxotrophic Marker

AbstractTrichoderma harzianum is a filamentous fungus used as a biological control agent for agricultural pests. Genes of this microorganism have been studied, and their applications are patented for use in biofungicides and plant breeding strategies. Gene editing technologies would be of great importance for genetic characterization of this species, but have not yet been reported. This work describes mutants obtained with an auxotrophic marker in this species using the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/ Cas (CRISPR-associated) system. For this, sequences for a guide RNA and Cas9 overexpression were inserted via biolistics, and the sequencing approach confirmed deletions and insertions at the pyr4 gene. Phenotypic characterization demonstrated a reduction in the growth of mutants in the absence of uridine, as well as resistance to 5-fluorotic acid. In addition, the gene disruption did not reduce mycoparasitc activity against phytopathogens. Thus, target disruption of the pyr4 gene in T. harzianum using the CRISPR/Cas9 system was demonstrated, and it was also shown that endogenous expression of the system did not interfere with the biological control activity of pathogens. This work is the first report of CRISPR Cas9-based editing in this biocontrol species, and the mutants expressing Cas9 have potential for the generation of useful technologies in agricultural biotechnology.

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