Bioactive compounds synthesized by non-ribosomal peptide synthetases and type-I polyketide synthases discovered through genome-mining and metagenomics

2012 ◽  
Vol 34 (8) ◽  
pp. 1393-1403 ◽  
Author(s):  
Katerina Nikolouli ◽  
Dimitris Mossialos
Keyword(s):  
Bioactive Compounds ◽  
Genome Mining ◽  
Polyketide Synthases ◽  
Type I ◽  
Peptide Synthetases

scholarly journals A 3-hydroxy-3-methylglutaryl-CoA synthase-based probe for the discovery of the acyltransferase-less type I polyketide synthases

2019 ◽  
Author(s):  
Haoyu Liang ◽  
Lin Jiang ◽  
Qiyun Jiang ◽  
Jie Shi ◽  
Jingxi Xiang ◽  
...  
Keyword(s):  
Natural Products ◽  
Network Analysis ◽  
In Silico ◽  
Genome Mining ◽  
Evolutionary Relationship ◽  
Soil Samples ◽  
Polyketide Synthases ◽  
Type I ◽  
Mining Sequence ◽  
Tailoring Enzymes

ABSTRACTAcyltransferase (AT)-less type I polyketide synthases (PKSs) produce complex natural products due to the presence of many unique tailoring enzymes. The 3-hydroxy-3-methylglutaryl coenzyme A synthases (HCSs) are responsible for β-alkylation of the growing polyketide intermediates in AT-less type I PKSs. In this study, we discovered a large group of HCSs, closely associated with the characterized and orphan AT-less type I PKSs through in silico genome mining, sequence and genome neighborhood network analysis. Using HCS-based probes, the survey of 1207 inhouse strains and 18 soil samples from different geological locations revealed the vast diversity of HCS-containing AT-less type I PKSs. The presence of HCSs in many AT-less type I PKSs suggests their co-evolutionary relationship. Our study should inspire future efforts to discover new polyketide natural products from AT-less type I PKSs.

Genome mining and characterisation of multiple bioactive compounds from a Burkholderia gladioli isolate collection

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
C Jones ◽  
MJ Bull ◽  
M Jenner ◽  
L Song ◽  
Y Dashti ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Genome Mining ◽  
Burkholderia Gladioli

scholarly journals Anabaenopeptins: What We Know So Far

Toxins ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 522
Author(s):  
Patrick Romano Monteiro ◽  
Samuel Cavalcante do Amaral ◽  
Andrei Santos Siqueira ◽  
Luciana Pereira Xavier ◽  
Agenor Valadares Santos
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Inhibitory Activity ◽  
Important Class ◽  
Biotechnological Applications ◽  
Peptide Synthetases ◽  
Ecological Roles ◽  
Cyclic Hexapeptides ◽  
Key Features

Cyanobacteria are microorganisms with photosynthetic mechanisms capable of colonizing several distinct environments worldwide. They can produce a vast spectrum of bioactive compounds with different properties, resulting in an improved adaptative capacity. Their richness in secondary metabolites is related to their unique and diverse metabolic apparatus, such as Non-Ribosomal Peptide Synthetases (NRPSs). One important class of peptides produced by the non-ribosomal pathway is anabaenopeptins. These cyclic hexapeptides demonstrated inhibitory activity towards phosphatases and proteases, which could be related to their toxicity and adaptiveness against zooplankters and crustaceans. Thus, this review aims to identify key features related to anabaenopeptins, including the diversity of their structure, occurrence, the biosynthetic steps for their production, ecological roles, and biotechnological applications.

scholarly journals The architectures of iterative type I PKS and FAS

2018 ◽  
Vol 35 (10) ◽  
pp. 1046-1069 ◽  
Author(s):  
Dominik A. Herbst ◽  
Craig A. Townsend ◽  
Timm Maier
Keyword(s):  
Fatty Acid ◽  
Polyketide Synthases ◽  
Type I ◽  
Fatty Acid Synthases

The architectures of fatty acid synthases and iterative polyketide synthases are remarkably divergent despite their related biosynthetic logics.

A novel group of type I polyketide synthases (PKS) in animals and the complex phylogenomics of PKSs

Gene ◽  
2007 ◽  
Vol 392 (1-2) ◽  
pp. 47-58 ◽  
Author(s):  
Todd A. Castoe ◽  
Tricia Stephens ◽  
Brice P. Noonan ◽  
Cristina Calestani
Keyword(s):  
Polyketide Synthases ◽  
Type I

scholarly journals Both modular and single-domain Type I polyketide synthases are expressed in the brevetoxin-producing dinoflagellate, Karenia brevis (Dinophyceae)

2017 ◽  
Vol 53 (6) ◽  
pp. 1325-1339 ◽  
Author(s):  
Frances M. Van Dolah ◽  
Gurjeet S. Kohli ◽  
Jeanine S. Morey ◽  
Shauna A. Murray
Keyword(s):  
Karenia Brevis ◽  
Polyketide Synthases ◽  
Single Domain ◽  
Type I ◽  
Domain Type

scholarly journals Analysis of the complete genome sequence of a marine-derived strainStreptomycessp. S063 CGMCC 14582 reveals its biosynthetic potential to produce novel anti-complement agents and peptides

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6122 ◽  
Author(s):  
Liang-Yu Chen ◽  
Hao-Tian Cui ◽  
Chun Su ◽  
Feng-Wu Bai ◽  
Xin-Qing Zhao
Keyword(s):  
Bioactive Compounds ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Dna Hybridization ◽  
Complete Genome ◽  
System Analysis ◽  
Genome Mining ◽  
Biosynthetic Gene Cluster ◽  
Genome Comparison ◽  
Genome Sequences

Genome sequences of marine streptomycetes are valuable for the discovery of useful enzymes and bioactive compounds by genome mining. However, publicly available complete genome sequences of marine streptomycetes are still limited. Here, we present the complete genome sequence of a marine streptomyceteStreptomycessp. S063 CGMCC 14582. Species delineation based on the pairwise digital DNA-DNA hybridization and genome comparison ANI (average nucleotide identity) value showed thatStreptomycessp. S063 CGMCC 14582 possesses a unique genome that is clearly different from all of the other available genomes. Bioactivity tests showed thatStreptomycessp. S063 CGMCC 14582 produces metabolites with anti-complement activities, which are useful for treatment of numerous diseases that arise from inappropriate activation of the human complement system. Analysis of the genome reveals no biosynthetic gene cluster (BGC) which shows even low similarity to that of the known anti-complement agents was detected in the genome, indicating thatStreptomycessp. S063 CGMCC 14582 may produce novel anti-complement agents of microbial origin. Four BGCs which are potentially involved in biosynthesis of non-ribosomal peptides were disrupted, but no decrease of anti-complement activities was observed, suggesting that these four BGCs are not involved in biosynthesis of the anti-complement agents. In addition, LC-MS/MS analysis and subsequent alignment through the Global Natural Products Social Molecular Networking (GNPS) platform led to the detection of novel peptides produced by the strain.Streptomycessp. S063 CGMCC 14582 grows rapidly and is salt tolerant, which benefits efficient secondary metabolite production via seawater-based fermentation. Our results indicate thatStreptomycessp. S063 has great potential to produce novel bioactive compounds, and also is a good host for heterologous production of useful secondary metabolites for drug discovery.

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