scholarly journals Erratum to: Peroxisome proliferator-activated receptor gamma activates fas ligand gene promoter inducing apoptosis in human breast cancer cells

2017 ◽  
Vol 166 (1) ◽  
pp. 325-326
Author(s):  
Daniela Bonofiglio ◽  
Sabrina Gabriele ◽  
Saveria Aquila ◽  
Hongyan Qi ◽  
Maria Belmonte ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Fas Ligand ◽  
Gene Promoter ◽  
Peroxisome Proliferator ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Peroxisome Proliferator Activated Receptor

scholarly journals WY-14643 Regulates CYP1B1 Expression through Peroxisome Proliferator-Activated Receptor α-Mediated Signaling in Human Breast Cancer Cells

2019 ◽  
Vol 20 (23) ◽  
pp. 5928 ◽  
Author(s):  
Hwang ◽  
Won ◽  
Jin ◽  
Lee ◽  
Pham ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Peroxisome Proliferator ◽  
Mrna Levels ◽  
Human Breast ◽  
Peroxisome Proliferator Activated Receptor ◽  
Cytochrome P450 1B1 ◽  
Peroxisome Proliferator Response Element

Human cytochrome P450 1B1 (CYP1B1)-mediated biotransformation of endobiotics and xenobiotics plays an important role in the progression of human breast cancer. In this study, we investigated the effects of WY-14643, a peroxisome proliferator-activated receptor α (PPARα) agonist, on CYP1B1 expression and the related mechanism in MCF7 breast cancer cells. We performed quantitative reverse transcription-polymerase chain reaction, transient transfection, and chromatin immunoprecipitation to evaluate the effects of PPARα on peroxisome proliferator response element (PPRE)-mediated transcription. WY-14643 increased the protein and mRNA levels of CYP1B1, as well as promoter activity, in MCF-7 cells. Moreover, WY-14643 plus GW6471, a PPARα antagonist, significantly inhibited the WY-14643-mediated increase in CYP1B1 expression. PPARα knockdown by a small interfering RNA markedly suppressed the induction of CYP1B1 expression by WY-14643, suggesting that WY-14643 induces CYP1B1 expression via a PPARα-dependent mechanism. Bioinformatics analysis identified putative PPREs (−833/−813) within the promoter region of the CYP1B1 gene. Inactivation of these putative PPREs by deletion mutagenesis suppressed the WY-14643-mediated induction of CYP1B1 promoter activation. Furthermore, WY-14643 induced PPARα to assume a form capable of binding specifically to the PPRE-binding site in the CYP1B1 promoter. Our findings suggest that WY-14643 induces the expression of CYP1B1 through activation of PPARα.

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