Gatekeepers’ perceptions of the quality and availability of services for breast and cervical cancer patients in the English-speaking Windward Islands: an exploratory investigation

2017 ◽  
Vol 28 (11) ◽  
pp. 1195-1206 ◽  
Author(s):  
Kamilah B. Thomas-Purcell ◽  
Will L. Tarver ◽  
Christine Richards ◽  
Marva Primus-Joseph
2021 ◽  
Vol 104 (6) ◽  
pp. 902-910

Background: Detection of human papillomavirus (HPV) in breast cancer patients has suggested a possible contributing role of the virus in cancer progression in this population. Objective: To investigate the presence of HPVs in Thai breast cancer patients and examine the potential activities of HPVs identified in both breast and cervical cancer cells. Materials and Methods: Fifty-five breast cancer tissues from Thai patients were subjected to HPV detection using PCR-EIA and DNA sequencing. Detection of HPV E6 proteins in sample tissues was examined by fluorescence immunohistochemistry. Cervical and two types of breast cancer cell lines expressing HPV oncogenes were established. The separate and combination of HPV oncoproteins activity for p53 degradation and specific gene regulation were investigated using western blot analysis and qPCR. Cell proliferation was assessed by MTT assay. Results: Twenty-two percent (10/45) of invasive breast cancers were found infected with various high-risk HPV types, with HPV58 E6D4G/E7T20IG63S being the most common variant. The percentage of HPV58 alone was approximately 50% (5/10) of all HPV positive samples. Similar potential oncogenic activity for this variant was observed in breast and cervical cancer cells. A separate analysis of single or combination of 58E6 (prototype or E6D4G) with 58E7 (prototype or E7T20IG63S) demonstrated that co-expression of 58E7T20IG63S with 58E6 (either prototype or E6D4G) significantly promoted cell proliferation compared to prototype 58E6/E7. Enhanced proliferation was mediated through elevated p53 degradation and reduced p21 expression. While p53 degradation activity was greatly diminished from E6 with D4G mutation, co-expression with E7T20IG63S cooperated to enhance degradation of p53 and promoted cell growth. Conclusion: HPV58 E6D4G/E7T20IG63S was the most HPV oncogene variant detected in Thai breast cancer patients. This variant exhibited in promoting cell proliferation and p53 degradation. A cooperative effect was observed in combination of HPV oncoproteins. Keywords: Human papillomavirus type 58; oncogene variant; breast cancer; Thai patients; altered cell growth


2005 ◽  
Vol 3 (3) ◽  
pp. 180-192 ◽  
Author(s):  
Marjorie Kagawa-Singer ◽  
Liane Wong ◽  
Sara Shostak ◽  
Chantal Raymer Walsh ◽  
Rod Lew

Introduction: Early detection and screening are the most effective means to reduce cancer mortality in all populations. Asian American (AA) women have among the lowest rates in aggregate for use of early detection, and screening practices of all ethnic populations. The only nationally disaggregated populationbased data on these ethnic groups at the time of the study was the 1993-1994 National Health Interview Survey (NHIS) of English speaking AA, but 70% of the AA population is non-English speaking. Our study presents heretofore unavailable data for cancer screening for monolingual AA women for a comparable time period in California between 1992 and 1994, prior to initiation of the state and Federal programs targeting this group of women. Methods: Retrospective chart reviews of randomly selected medical records were conducted for the breast and cervical cancer screening practices of low-income, non-English speaking Chinese, Korean, and Thai women attending ethnic specific community-based health clinics. All women seen in the clinics between 1992-1994 who were within the appropriate screening age categories were eligible. Results: Asian American women utilizing ethnic specific clinics had equal to or better screening rates for mammography and Pap tests than mainstream services for English-speaking AA women in a national survey. These screening rates, however, were still well below nationally recommended screening rates for breast and cervical cancer according to Healthy People 2000 or 2010 goals. Conclusion: These data support the effectiveness of Community Based Clinics (CBOs) to reach these hard to reach women and address the call for the elimination of health disparities. In addition, we compare our findings with national data to highlight within group variations.


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