scholarly journals Fabrication of dual drug loaded bilayered chitosan based composite scaffolds as osteochondral substitutes and evaluation of in vitro cell response using the MC3T3 pre-osteoblast cell line

Cellulose ◽  
2019 ◽  
Vol 27 (4) ◽  
pp. 2253-2266
Author(s):  
Muhammad Samie ◽  
Muhammad Arfat Yameen ◽  
Hafiza Fakhera Ikram ◽  
Haffsah Iqbal ◽  
Aqif Anwar Chaudhry ◽  
...  

2018 ◽  
Vol 101 (3) ◽  
pp. 1827-1833 ◽  
Author(s):  
J.L. Zhang ◽  
X. Han ◽  
Y.J. Shan ◽  
L.W. Zhang ◽  
M. Du ◽  
...  


2013 ◽  
Vol 7 (1) ◽  
pp. 41-48 ◽  
Author(s):  
A Shahrulazua ◽  
AR Samsudin ◽  
MA Iskandar ◽  
AS Amran


2009 ◽  
Vol 24 (4) ◽  
pp. 343-356 ◽  
Author(s):  
Yu-Ting Huang ◽  
Ching-Yu Lai ◽  
Shyh-Liang Lou ◽  
Jui-Ming Yeh ◽  
Wen-Hsiung Chan




2014 ◽  
Vol 87 (3-4) ◽  
pp. 111-118 ◽  
Author(s):  
Yuko Akiyama ◽  
Yoshikazu Mikami ◽  
Eri Watanabe ◽  
Nobukazu Watanabe ◽  
Taku Toriumi ◽  
...  


2020 ◽  
Vol 21 (6) ◽  
pp. 1995-2013 ◽  
Author(s):  
Rémi G. Tilkin ◽  
Nicolas Régibeau ◽  
Stéphanie D. Lambert ◽  
Christian Grandfils


2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Hiroshi Kobayashi ◽  
Takayuki Endoh ◽  
Yushi Uchida ◽  
Masakazu Tazaki ◽  
Kenji Sueishi


2008 ◽  
Vol 34 (4) ◽  
pp. 196-202 ◽  
Author(s):  
Wihaskoro Sosroseno ◽  
Erwan Sugiatno ◽  
Abdul Rani Samsudin ◽  
Mohd Fikri Ibrahim

Abstract The aim of the present study was to test the hypothesis that the proliferation of a human osteoblast cell line (HOS cells) stimulated with hydroxyapatite (HA) may be regulated by nitric oxide (NO). The cells were cultured on the surface of HA. Medium or cells alone were used as controls. L-arginine, D-arginine, 7-NI (an nNOS inhibitor), L-NIL (an iNOS inhibitor), L-NIO (an eNOS inhibitor) or carboxy PTIO, a NO scavenger, was added in the HA-exposed cell cultures. The cells were also precoated with anti-human integrin αV antibody. The levels of nitrite were determined spectrophotometrically. Cell proliferation was assessed by colorimetric assay. The results showed increased nitrite production and cell proliferation by HA-stimulated HOS cells up to day 3 of cultures. Anti-integrin αV antibody, L-NIO, or carboxy PTIO suppressed, but L-arginine enhanced, nitrite production and cell proliferation of HA-stimulated HOS cells. The results of the present study suggest, therefore, that interaction between HA and HOS cell surface integrin αV molecule may activate eNOS to catalyze NO production which, in turn, may regulate the cell proliferation in an autocrine fashion.



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