A novel, simple and rapid nondenaturing FISH (ND-FISH) technique for the detection of plant telomeres. Potential used and possible target structures detected

Telomeres i.e., termini of the eukaryotic chromosomes protect chromosomes during DNA replication. Shortening of telomeres, either due to stress or ageing is related to replicative cellular senescence. There is little information on the effect of biotechnological methods, such as tissue culture via somatic embryogenesis (SE) or cryopreservation on plant telomeres, even if these techniques are widely applied. The aim of the present study was to examine telomeres of Norway spruce (Picea abies (L.) Karst.) during SE initiation, proliferation, embryo maturation, and cryopreservation to reveal potential ageing or stress-related effects that could explain variation observed at SE process. Altogether, 33 genotypes from 25 families were studied. SE initiation containing several stress factors cause telomere shortening in Norway spruce. Following initiation, the telomere length of the embryogenic tissues (ETs) and embryos produced remains unchanged up to one year of culture, with remarkable genotypic variation. Being prolonged in vitro culture can, however, shorten the telomeres and should be avoided. This is achieved by successful cryopreservation treatment preserving telomere length. Somatic embryo production capacity of the ETs was observed to vary a lot not only among the genotypes, but also from one timepoint to another. No connection between embryo production and telomere length was found, so this variation remains unexplained.

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Combination of Direct Viable Count and Fluorescent In Situ Hybridization (DVC-FISH) as a Potential Method for Identifying Viable Vibrio parahaemolyticus in Oysters and Mussels

Foods ◽

10.3390/foods10071502 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1502

Author(s):

Jorge García-Hernández ◽

Manuel Hernández ◽

Yolanda Moreno

Keyword(s):

In Situ Hybridization ◽

Vibrio Parahaemolyticus ◽

Fluorescent In Situ Hybridization ◽

Potential Method ◽

Viable Count ◽

Hybridization Technique ◽

Fish Technique ◽

Viable Cells

Vibrio parahaemolyticus is a human food-borne pathogen with the ability to enter the food chain. It is able to acquire a viable, non-cultivable state (VBNC), which is not detected by traditional methods. The combination of the direct viable count method and a fluorescent in situ hybridization technique (DVC-FISH) makes it possible to detect microorganisms that can present VBNC forms in complex samples The optimization of the in vitro DVC-FISH technique for V. parahaemolyticus was carried out. The selected antibiotic was ciprofloxacin at a concentration of 0.75 μg/mL with an incubation time in DVC broth of 5 h. The DVC-FISH technique and the traditional plate culture were applied to detect and quantify the viable cells of the affected pathogen in artificially contaminated food matrices at different temperatures. The results obtained showed that low temperatures produced an important logarithmic decrease of V. parahaemolyticus, while at 22 °C, it proliferated rapidly. The DVC-FISH technique proved to be a useful tool for the detection and quantification of V. parahaemolyticus in the two seafood matrices of oysters and mussels. This is the first study in which this technique has been developed to detect viable cells for this microorganism.

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A combined PRINS-FISH technique for simultaneous localisation of DNA sequences on plant chromosomes

Biologia Plantarum ◽

10.1023/a:1001847505353 ◽

1998 ◽

Vol 41 (2) ◽

pp. 293-296 ◽

Cited By ~ 2

Author(s):

M. Kubalakova ◽

M. Nouzova ◽

M. Dolezelova ◽

J. Macas ◽

J. Dolezel

Keyword(s):

Dna Sequences ◽

Fish Technique ◽

Plant Chromosomes

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Chromosomal aberrations in bilharzial bladder cancer using fish technique

European Journal of Cancer ◽

10.1016/s0959-8049(99)81840-1 ◽

1999 ◽

Vol 35 ◽

pp. S352

Author(s):

M.Khaled Hussein ◽

M.S. Aly

Keyword(s):

Bladder Cancer ◽

Chromosomal Aberrations ◽

Fish Technique

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Application of the FISH Technique to Visualize Sex Chromosomes in Domestic Cat Spermatozoa

Animals ◽

10.3390/ani11072106 ◽

2021 ◽

Vol 11 (7) ◽

pp. 2106

Author(s):

Barbara Kij-Mitka ◽

Halina Cernohorska ◽

Svatava Kubickova ◽

Sylwia Prochowska ◽

Wojciech Niżański ◽

...

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Sex Chromosomes ◽

Chromosomal Abnormalities ◽

Molecular Cytogenetics ◽

Molecular Probes ◽

Domestic Cat ◽

Fish Technique ◽

Y Chromosomes

Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.

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Frequency of recombinant and nonrecombinant products of pericentric inversion of chromosome 1 in sperm nuclei of carrier: By FISH technique

Molecular Reproduction and Development ◽

10.1002/mrd.10325 ◽

2003 ◽

Vol 66 (1) ◽

pp. 67-71 ◽

Cited By ~ 11

Author(s):

Tahsin Yakut ◽

Hasan Acar ◽

�nal Egeli ◽

Yal�in Kimya

Keyword(s):

Pericentric Inversion ◽

Chromosome 1 ◽

Fish Technique ◽

Sperm Nuclei

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EFEKTIVITAS TEKNIK MANGKUK IKAN ATAU AKUARIUM (FISH BOWL) UNTUK MENINGKATKAN HASIL BELAJAR IPS PADA SISWA KELAS V A SDN 16 CAKRANEGARA TAHUN PELAJARAN 2016/2017

JKKP (Jurnal Kesejahteraan Keluarga dan Pendidikan) ◽

10.21009/jkkp.051.08 ◽

2018 ◽

Vol 5 (1) ◽

pp. 82-94

Author(s):

Siti Istiningsih ◽

Ni Komang Sri Widari ◽

Nur Hasanah

Keyword(s):

Social Learning ◽

Active Learning ◽

Cooperative Learning ◽

Learning Outcomes ◽

Action Observation ◽

Fifth Grade ◽

Card Sort ◽

Fish Technique ◽

Fifth Grade Students ◽

Teacher Centered

Background of this research is the low social learning outcomes on students fifth grade at SDN 16 Cakranegara, it is caused by learning process focus on teaccher (Teacher Centered) and they used monoton method. Teacher usually teach only usuhg speech method and they didnâ€™t support by the appropriate facilities. The problem in this research is how the efectivness of bowl fish teachnique could improve social learning outcomes on fifth grade students at SDN 16 Cakranegara in 2016 to 2017 ? The purpose is to improve social learning outcomes on fifth grade students in 2016 to 2017 by using bowl fish technique or aquarum. This is a base calssrom research in two cydes each cycle consist of planning, implementation, action, observation,and evaluation, reflection as well. The method collecting data are observation, documentation and test. In the frist cycle the result is on average 75,375 with percentage 70,83% and the scores for activities of students are 53 categories active enough. In second cycle the students having an increase on average 82,791 categories active. The conelusion is the efectivness of bowl fish technique could improve social learing outcomes on fifth grade students at SDN 16 Cakranegara in 2016 to 2017. Key Word : The efectivness, techniques of â€œbowl fish technique or aquariumâ€, and social learing out comes. Abstrak Penelitian ini dilatarbelakangi rendahnya hasil belajar IPS siswa kelas V A di SDN 16 Cakranegara. Hal ini disebabkan pembelajaran masih terpusat pada guru (Teacher Centered) dan teknik serta metode yang digunakan monoton. Guru biasanya hanya mengajar dengan metode ceramah saja, dan tidak di dukung dengan sarana dan prasarana yang memadai. Masalah penelitian ini adalah bagaimanakah Efektivitas Teknik Mangkuk Ikan atau Akuarium (Fish Bowl) dapat meningkatkan hasil belajar IPS siswa kelas V A SDN 16 Cakranegara Tahun Pelajaran 2016/2017?. Tujuan penelitian ini adalah untuk meningkatkan hasil belajar IPS siswa kelas V A SDN 16 Cakra Negara Tahun Pelajaran 2016/2017 dengan menerapkan teknik Mangkuk Ikan Atau Akuarium (Fish Bowl). Jenis penelitian ini adalah penelitian tindakan kelas yang dilaksanakan dalam 2 siklus. Setiap siklus terdiri dari tahap perencanaan, pelaksanaan, tindakan, observasi, dan evaluasi serta refleksi. Metode yang digunakan dalam mengumpulkan data dalam penelitian ini adalah metode observasi, dokumentasi dan tes. Pada siklus I diperoleh nilai rata-rata 75,375 dengan ketuntasan klasikal 70,83% dan skor rata-rata aktivitas siswa 53 dengan kategori cukup aktif. Pada siklus II data hasil belajar dan aktivitas siswa mengalami peningkatan yakni rata-rata 82,791 dengan ketuntasan klasikal 87,5% dan skor rata-rata aktivitas belajar siswa 58,5 dengan kriteria aktif. Dengan demikian dapat disimpulkan bahwa Efektivitas Teknik Mangkuk Ikan atau Akuarium (Fish Bowl) dapat meningkatkan hasil belajar IPS siswa kelas V A SDN 16 Cakranegara Tahun Pelajaran 2016/2017. Kata Kunci: Efektivitas, Teknik â€œMangkuk Ikan atau Akuarium (Fish Bowl)â€, hasil belajar IPS. References Asmani, M. Jamal. 2011. 7 Tips Aplikasi Pakem. Jogjakarta: DIVA Press. Aries, E. 2011. Assesmen Dan Evaluasi. Yogyakarta: Adytia Media Publising. Arikunto, S. 2010. Prosedur Penelitian. Jakarta: Rineka Cipta. Azizah R. Anindita. 2014. Penggunaan Metode Active Learning Tipe Card Sort Untuk Meningkatkan Keaktifan dan Prestasi Belajar IPS Siswa Kelas IV SD Negeri Sendangsari. Skripsi. Pendidikan Guru Sekolah Dasar Universitas Negeri Yogyakarta. https://www.google.com, di akses pada tanggal 25 januari 2017. Alma, Buchari, dkk. 2014. Guru Professional. Bandung: Alfabeta. Huda, Miftahul. 2015.Cooperative Learning. Yogyakarta: Pustaka Pelajar. Khotimah, Husnil. 2011. Penerapan Metode Pembelajaran Fishbowl Untuk Meningkatkan Aktivitas Belajar Sains Pada Siswa Kelas IV SDN 002 Pantai Cermin Kecamatan Tapung Kabupaten Kampar. Skripsi. Pendidikan Guru Madrasah Ibtidaiyah Universitras Islam Negeri Sultan Syarif Kasim Riau. https://www.google.com, diakses tanggal 17 Desember 2016. Nurkancana, Wayan dan Sunartana. 1990. Evaluasi Hasil Belajar. Surabaya: Usaha Nasional. Qurâ€™anni,E Afriliya. 2013. Pengaruh Metode Team Quiz Terhadap Minat Belajar dan Pencapaian Kompetensi Menghadapi Situasi Darurat Pada Mata Pelajaran K3lh Di Smk Negeri 2 Godean. Skripsi. Program Studi Pendidikan Teknik Busana Universitas Negeri Yogyakarta. https://www.google.com, diakses tanggal 25 januari 2017. Sanjaya, Wina. 2006. Strategi Pembelajaran. Jakarta: Kencana. Sari, Melda, Supriyadi, Dan Sudirman A. 2014. Penerapan Strategi Active Learning Tipe Everyone Is Ateacher Here Untuk Meningkatkan Hasil Belajar. Jurnal. Fakultas Keguruan Dan Ilmu Pendidikan Universitas Lampung. https://www.google.com, diakses tanggal 25 januari 2017. Silberman, Mel. 2010. 101 Cara Pelatihan & Pembelajaran Aktif. Jakarta Barat: Indeks. Sudjana, Nana. 2014. Dasar-Dasar Proses Belajar Mengajar. Bandung: Sinar Baru Algensindo. Suprijono, Agus. 2014. Cooperative Learning. Yogyakarta: Pustaka Pelajar. Susanto. 2013. Teori Belajar & Pembelajaran Di Sekolah Dasar. Jakarta: Kencana. Trianto. 2011. Model Pembelajaran Inovatif Berorientasi Kontroktivis. Jakarta: Prestasi Pustaka Uno, B Hamzah dan Mohamad, Nurdin. 2012. Belajar dengan Pendekatan PAILKEM.Jakarta: Bumi Aksara. Utami, B. Rahmawati. 2012. Keefektifan Metode Fishbowl Terhadap Pembelajaran Berdiskusi Pada Siswa Kelas Viii Smp Negeri 5 Sleman. Skripsi. Pendidikan Bahasa Dan Sastra Indonesia Universitas Negeri Yogyakarta https://www.google.com, diakses tanggal 26 november 2016. Wahidah, S, Susan. 2014. Penerpan Metode â€œSTEAVES BERHTOâ€ (STUDENT TEAM-ACHIEVEMENT DIVISIONS DAN NUMBERED HEADS TOGETHER) untuk meningkatkan hasil belajar IPS siswa keas IVC SDN 1 kediri Tahun Pelajaran 2013/2014. Skripsi. Keguruan dan Ilmu Pendidikan, Pendidikan Guru Sekolah Dasar. Universitas Mataram. Warsono dan Hariyanto. 2013. Pembelajaran Aktif. Bandung: Remaja Rosdakarya.

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