Transforming Growth Factor beta1 Gene Variation Leu10Pro Affects Secretion and Function in Hepatic Cells

2012 ◽  
Vol 57 (11) ◽  
pp. 2901-2909 ◽  
Author(s):  
Xing Gu ◽  
Xin Ji ◽  
Le-Hua Shi ◽  
Chang-Hong Yi ◽  
Yun-Peng Zhao ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Beta1 ◽  
Gene Variation ◽  
Hepatic Cells ◽  
And Function

scholarly journals Fibronectin is a survival factor for differentiated osteoblasts

1998 ◽  
Vol 111 (10) ◽  
pp. 1385-1393 ◽  
Author(s):  
R.K. Globus ◽  
S.B. Doty ◽  
J.C. Lull ◽  
E. Holmuhamedov ◽  
M.J. Humphries ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Nuclear Condensation ◽  
Transforming Growth Factor Beta1 ◽  
Amino Terminal ◽  
Characteristic Features ◽  
Induced Apoptosis ◽  
Carboxy Terminal ◽  
And Function

The skeletal extracellular matrix produced by osteoblasts contains the glycoprotein fibronectin, which regulates the adhesion, differentiation and function of various adherent cells. Interactions with fibronectin are required for osteoblast differentiation in vitro, since fibronectin antagonists added to cultures of immature fetal calvarial osteoblasts inhibit their progressive differentiation. To determine if fibronectin plays a unique role in fully differentiated osteoblasts, cultures that had already formed mineralized nodules in vitro were treated with fibronectin antagonists. Fibronectin antibodies caused >95% of the cells in the mature cultures to display characteristic features of apoptosis (nuclear condensation, apoptotic body formation, DNA laddering) within 24 hours. Cells appeared to acquire sensitivity to fibronectin antibody-induced apoptosis as a consequence of differentiation, since antibodies failed to kill immature cells and the first cells killed were those associated with mature nodules. Intact plasma fibronectin, as well as fragments corresponding to the amino-terminal, cell-binding, and carboxy-terminal domains of fibronectin, independently induced apoptosis of mature (day-13), but not immature (day-4), osteoblasts. Finally, transforming growth factor-beta1 partially protected cells from the apoptotic effects of fibronectin antagonists. Thus, in the course of maturation cultured osteoblasts switch from depending on fibronectin for differentiation to depending on fibronectin for survival. These data suggest that fibronectin, together with transforming growth factor-beta1, may affect bone formation, in part by regulating the survival of osteoblasts.

scholarly journals Serum level of transforming growth factor-beta1 in major idiopathic interstitial pneumonia

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Mohamed W. Zakaria ◽  
Reem I. El-Korashy ◽  
Samah Selim ◽  
Ingy Badawy ◽  
Kwashi J. Amum
Keyword(s):  
Growth Factor ◽  
Serum Level ◽  
Interstitial Pneumonia ◽  
Transforming Growth Factor ◽  
Idiopathic Interstitial Pneumonia ◽  
Transforming Growth Factor Beta1
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