scholarly journals The role of Caenorhabditis elegans sex-determination homologs, Mi-sdc-1 and Mi-tra-1 in Meloidogyne incognita

2021 ◽  
Author(s):  
Anil Baniya ◽  
Soumi Joseph ◽  
Larry Duncan ◽  
William Crow ◽  
Tesfamariam Mengistu
Keyword(s):  
Caenorhabditis Elegans ◽  
Sex Determination ◽  
Meloidogyne Incognita ◽  
Egg Production ◽  
Target Genes ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Root Knot Nematode ◽  
Model Free ◽  
Plant Parasitic

AbstractSex determination is a key developmental event in all organisms. The pathway that regulates sexual fate has been well characterized at the molecular level in the model free-living nematode Caenorhabditis elegans. This study aims to gain a preliminary understanding of sex-determining pathways in a plant-parasitic nematode Meloidogyne incognita, and the extent to which the roles of the sex determination genes are conserved in a hermaphrodite species, C. elegans, and plant-parasitic nematode species, M. incognita. In this study, we targeted two sex-determining orthologues, sdc-1 and tra-1 from M. incognita using RNA interference (RNAi). RNAi was performed by soaking second-stage juveniles of M. incognita in a solution containing dsRNA of either Mi-tra-1or Mi-sdc-1 or both. To determine the effect of RNAi of the target genes, the juveniles treated with the dsRNA were inoculated onto a susceptible cultivar of cowpea grown in a nutrient pouch at 28 °C for 5 weeks. The development of the nematodes was analyzed at different time points during the growth period and compared to untreated controls. Our results showed that neither Mi-sdc-1 nor Mi-tra-1 have a significant role in regulating sexual fate in M. incognita. However, the silencing of Mi-sdc-1 significantly delayed maturity to adult females but did not affect egg production in mature females. In contrast, the downregulation of Mi-tra-1 transcript resulted in a significant reduction in egg production in both single and combinatorial RNAi-treated nematodes. Our results indicate that M. incognita may have adopted a divergent function for Mi-sdc-1 and Mi-tra-1distinct from Caenorhabditis spp. However, Mi-tra-1 might have an essential role in female fecundity in M. incognita and is a promising dsRNA target for root-knot nematode (RKN) management using host-delivered RNAi.

Isolation of microsatellites from an enriched genomic library of the plant-parasitic nematode Meloidogyne incognita and their detection in other root-knot nematode species

2010 ◽  
Vol 129 (4) ◽  
pp. 501-505 ◽  
Author(s):  
Karine Mulet ◽  
Mireille Fargette ◽  
Myriam Richaud ◽  
Gwenaelle Genson ◽  
Philippe Castagnone-Sereno
Keyword(s):  
Meloidogyne Incognita ◽  
Parasitic Nematode ◽  
Genomic Library ◽  
Nematode Species ◽  
Plant Parasitic Nematode ◽  
Enriched Genomic Library ◽  
Root Knot Nematode ◽  
Plant Parasitic

Meloidogyne incognita (root-knot nematode).

2021 ◽  
Author(s):  
Jonathan D. Eisenback
Keyword(s):  
Meloidogyne Incognita ◽  
Yield Loss ◽  
Parasitic Nematode ◽  
Nematode Species ◽  
Plant Parasitic Nematode ◽  
Root Knot Nematode ◽  
Growth Effects ◽  
Wide Range ◽  
The World ◽  
Plant Parasitic

Abstract Introduction: The root knot nematode species, M. incognita, is the most widespread and probably the most serious plant parasitic nematode pest of tropical and subtropical regions throughout the world (Sasser, 1979). It occurs as a pest on a very wide range of crops. Most estimates of yield loss come from the use of nematicides and it should be noted that these can possibly cause other beneficial growth effects.

scholarly journals Meloidogyne incognita (root-knot nematode).

2021 ◽  
Author(s):  
Jonathan D. Eisenback
Keyword(s):  
Meloidogyne Incognita ◽  
Yield Loss ◽  
Parasitic Nematode ◽  
Nematode Species ◽  
Plant Parasitic Nematode ◽  
Root Knot Nematode ◽  
Growth Effects ◽  
Wide Range ◽  
The World ◽  
Plant Parasitic

Abstract The root knot nematode species, M. incognita, is the most widespread and probably the most serious plant parasitic nematode pest of tropical and subtropical regions throughout the world (Sasser, 1979). It occurs as a pest on a very wide range of crops. Most estimates of yield loss come from the use of nematicides and it should be noted that these can possibly cause other beneficial growth effects.

scholarly journals Construction of artificial microRNA expression vectors for inhibition of Minc16281 gene in root-knot nematode Meloidogyne incognita

2019 ◽  
Vol 18 (4) ◽  
pp. 62-69
Author(s):  
Phong V. Nguyen
Keyword(s):  
Meloidogyne Incognita ◽  
Parasitic Nematode ◽  
Expression Vectors ◽  
Parasitic Nematodes ◽  
Plant Parasitic Nematodes ◽  
Root Knot Nematode ◽  
Gene Encoding ◽  
Soybean Field ◽  
Genes Encoding ◽  
Plant Parasitic

Effectors have been identified to play a very important role in the parasitism of plant-parasitic nematode. To cope with this type of pathogen, many approaches of silencing genes encoding for effectors have been studied and promise to be an effective tool to create plant varieties resistant to plant-parasitic nematodes. In this study, the Minc16281 gene encoding a pioneer effector with unknown function was determined and cloned from a Meloidogyne incognita population isolated from soybean field (ID: MH315945.1). The nucleotide sequence of this gene showed 97% identity to its homolog in GenBank (ID: JK287445.1) used as the control strain in our research. To generate host-induced gene silencing constructs which can potentially silence the expression of Minc16281 gene, two artificial microRNAs were synthesized based on the miR319a structure of Arabidopsis thaliana and inserted into an expression vector in soybean. These microRNAs can be introduced into soybean to investigate the function of Minc16281 on parasitism of root-knot nematode.

Gene copy number variations as signatures of adaptive evolution in the parthenogenetic, plant‐parasitic nematode Meloidogyne incognita

2019 ◽  
Vol 28 (10) ◽  
pp. 2559-2572 ◽  
Author(s):  
Philippe Castagnone‐Sereno ◽  
Karine Mulet ◽  
Etienne G. J. Danchin ◽  
Georgios D. Koutsovoulos ◽  
Mégane Karaulic ◽  
...  
Keyword(s):  
Adaptive Evolution ◽  
Meloidogyne Incognita ◽  
Copy Number ◽  
Parasitic Nematode ◽  
Gene Copy Number ◽  
Copy Number Variations ◽  
Plant Parasitic Nematode ◽  
Gene Copy ◽  
Plant Parasitic

cDNA cloning and expression analysis of a calponin gene from the plant-parasitic nematode Meloidogyne incognita

2001 ◽  
Vol 112 (1) ◽  
pp. 149-152 ◽  
Author(s):  
Philippe Castagnone-Sereno ◽  
Frédéric Leroy ◽  
Pierre Abad
Keyword(s):  
Cdna Cloning ◽  
Expression Analysis ◽  
Meloidogyne Incognita ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Cloning And Expression ◽  
Plant Parasitic

Identification and analysis of a cuticular collagen-encoding gene from the plant-parasitic nematode meloidogyne incognita

Gene ◽  
1995 ◽  
Vol 155 (1) ◽  
pp. 145-146
Author(s):  
Walter Van der Eycken ◽  
Janice de Almeida Engler ◽  
Marc Van Montagu ◽  
Godelieve Gheysen
Keyword(s):  
Meloidogyne Incognita ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Encoding Gene ◽  
Plant Parasitic

scholarly journals IDENTIFIKASI FUSARIUM DAN NEMATODA PARASITIK YANG BERASOSIASI DENGAN PENYAKIT KUNING LADA DI KALIMANTAN BARAT (FUSARIUM IDENTIFICATION AND PLANT PARASITIC NEMATODE ASSOCIATED WITH PEPPER YELLOWING DISEASE IN WEST KALIMANTAN)

2016 ◽  
Vol 19 (1) ◽  
pp. 19
Author(s):  
Suryanti Suryanti ◽  
Bambang Hadisutrisno ◽  
Mulyadi Mulyadi ◽  
Jaka Widada
Keyword(s):  
Meloidogyne Incognita ◽  
Fusarium Solani ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Piper Nigrum ◽  
Diseased Plant ◽  
West Kalimantan ◽  
Leaf Yellowing ◽  
Yellowing Disease ◽  
Plant Parasitic

Pepper (Piper nigrum), known as the “King of Spices” is one of the most important spices. In the international market, Indonesian pepper has high selling value, due to its flavor characteristics. Pepper yellowing disease is one of the most important disease that caused the decrease of pepper production and become the main problem in the cultivation of pepper in West Kalimantan. This research was conducted to determine the major causal agent of leaf yellowing disease of pepper. The Fusarium associated with diseased plant were isolated from the symptomatic plant and nematodes were isolated from the root with leaf yellowing symptom. The Fusarium isolates were cultured on agar medium, and the nematode was cultured on tomato plant. From diseased pepper in West Kalimantan, it was isolated 4 Fusarium isolates and plant parasitic nematode Meloidogyne. The result showed that H isolate of Fusarium was the most virulent isolate and identified asFusarium solani. The Meloidogyne was identified by the female perenial patern.The nematode was identified as Meloidogyne incognita.Keywords: Fusarium solani, Meloidogyne incognita, pepper yellowing diseaseLada (Piper nigrum L.) merupakan salah satu jenis rempah penting yang telah dikenal sebagai “King of Spices”. Di pasar internasional, lada Indonesia mempunyai daya jual tinggi karena cita rasanya yang khas. Salah satu kendaladalam budidaya lada adalah adanya penyakit kuning lada dan sampai saat ini menjadi masalah utama pada pertanaman lada di Kalimantan Barat. Informasi tentang patogen utama yang berinteraksi dengan penyakit kuning lada masih sangat terbatas, sehingga penelitian ini bertujuan untuk mengidentifikasi patogen utama yang berasosiasi dengan penyakit kuning lada. Isolasi Fusarium dilakukan dari batang lada dan isolasi nematoda dilakukan dari akar lada yang bergejalapenyakit kuning di Kalimantan Barat. Fusarium hasil isolasi dikulturkan dalam medium agar, sedangkan nematoda hasil isolasi dikulturkan dalam akar tomat. Dari hasil isolasi berhasil didapatkan empat isolat Fusarium dan nematodaMeloidogyne. Identifikasi Fusarium dilakukan secara morfologis dan molekuler, dan identifikasi Meloidogyne dilakukan dengan menggunakan irisan bagian posterior nematoda betina. Dari hasil identifikasi diketahui bahwa patogenyang berasosiasi dengan penyakit kuning lada adalah Fusarium solani dan Meloidogyne incognita.Kata kunci: Fusarium solani, Meloidogyne incognita, penyakit kuning lada

Sign in / Sign up

Export Citation Format

Share Document