GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus

2014 ◽  
Vol 41 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Tomás Horacio Delgadin ◽  
Daniela Irina Pérez Sirkin ◽  
María Paula Di Yorio ◽  
Silvia Eda Arranz ◽  
Paula Gabriela Vissio
Keyword(s):  
Mrna Expression ◽  
Food Deprivation ◽  
Cichlid Fish ◽  
Deprivation Period ◽  
Growth Impairment ◽  
Igf I ◽  
Gh Receptors

Nutritional regulation of insulin-like growth factor-I mRNA expression in salmon tissues

1993 ◽  
Vol 139 (2) ◽  
pp. 243-252 ◽  
Author(s):  
C. Duan ◽  
E. M. Plisetskaya
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Food Deprivation ◽  
Mrna Levels ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Retarded Growth ◽  
Igf I ◽  
Growth Factor I ◽  
Plasma Gh

ABSTRACT In salmonids, nutritional insufficiency leads to retarded growth and reduced hepatic GH receptors, but increased circulating GH levels. To understand the endocrine mechanism underlying the retarded growth in starved fish better, we investigated the effect of food deprivation and refeeding on circulating levels of GH and insulin, as well as insulin-like growth factor-I (IGF-I) mRNA expression in different tissues of juvenile coho salmon (Oncorhynchus kisutch). Deprivation of food for 2–4 weeks resulted in cessation of growth and a significant decrease in condition factor (an indicator of fish body shape). No difference in circulating insulin or glucose levels were found between starved and fed fish, whereas starvation increased the plasma GH levels. After 4 weeks of starvation, the plasma GH level rose to 9 ng/ml, which was four times as high as that of the fed fish. In spite of elevated circulating GH, hepatic IGF-I mRNA levels were significantly reduced after 4 weeks of starvation. No significant difference in IGF-I mRNA levels of fed and starved fish was found in other tissues, including kidney, spleen, ovary, gill filament and gut. Two weeks of refeeding significantly increased hepatic IGF-I mRNA levels and growth and reduced plasma GH levels. These results suggest that food deprivation primarily reduces IGF-I mRNA expression in the liver which results, most probably, in a decline in systemic IGF-I levels and consequently leads to the retarded growth of salmon. Journal of Endocrinology (1993) 139, 243–252

Effects of refeeding of undernourished and insulin treatment of diabetic neonatal rats on IGF and IGFBP

1996 ◽  
Vol 271 (2) ◽  
pp. E223-E231 ◽  
Author(s):  
L. Goya ◽  
F. Rivero ◽  
M. A. Martin ◽  
R. Arahuetes ◽  
E. R. Hernandez ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Growth Factor ◽  
Mrna Expression ◽  
Insulin Treatment ◽  
Diabetic Rats ◽  
Insulin Like Growth Factor ◽  
Neonatal Rats ◽  
Igf I ◽  
Serum Gh

The effect of refeeding and insulin treatment of undernourished and diabetic neonatal rats, respectively, on the regulation of insulin-like growth factor (IGF) and insulin-like growth factor binding protein (IGFBP) was investigated. The changes in body weight, insulinemia, glycemia, serum IGF-I, and growth hormone (GH) as well as the increase of the 30-kDa IGFBP in undernourished and diabetic neonatal rats previously shown elsewhere were reversed by refeeding and insulin treatment, respectively. Also, changes in liver mRNA expression of IGF-I and-II and IGFBP-1 and -2 were restored in refed undernourished and IGF-I and IGFBP-1 levels recovered in insulin-treated diabetic rats. However, serum GH was still below normal after rehabilitation in both situations. Thus the present results support the idea of a GH-independent IGF/ IGFBP regulation mediated by a balance of insulin and nutrients as has already been suggested in previous neonatal studies.

scholarly journals Effects of β‐hydroxy‐β‐methylbutyrate (HMB) on Muscle IGF‐I and MGF mRNA Expression in Aged Female Rats during 10‐Week Resistance Training

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Young‐Min Park ◽  
Sang‐Rok Lee ◽  
Jacob M. Wilson ◽  
Paul C. Henning ◽  
Carlos Ugrinowitsch ◽  
...  
Keyword(s):  
Resistance Training ◽  
Mrna Expression ◽  
Female Rats ◽  
Igf I

Divergence between hepatic insulin-like growth factor (IGF)-I mRNA expression and IGF-I serum levels in Leishmania (Leishmania) infantum chagasi-infected dogs

2013 ◽  
Vol 151 (1-2) ◽  
pp. 163-167
Author(s):  
Flaviane A. Pinho ◽  
Nilton A. Magalhães ◽  
Kleverton R. Silva ◽  
Aline A. Carvalho ◽  
Fernando L.L. Oliveira ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Leishmania Infantum ◽  
Serum Levels ◽  
Insulin Like Growth Factor ◽  
Igf I ◽  
Leishmania Infantum Chagasi

scholarly journals Effect of mechanical loading on insulin-like growth factor-I gene expression in rat tibia

2007 ◽  
Vol 192 (1) ◽  
pp. 131-140 ◽  
Author(s):  
Christianne M A Reijnders ◽  
Nathalie Bravenboer ◽  
Annechien M Tromp ◽  
Marinus A Blankenstein ◽  
Paul Lips
Keyword(s):  
Bone Formation ◽  
Mrna Expression ◽  
Mechanical Loading ◽  
Mechanical Stimulation ◽  
Molecular Mechanisms ◽  
Bone Marrow Cells ◽  
Mechanical Stimuli ◽  
Igf I ◽  
Female Wistar Rats ◽  
Tibia Shaft

Mechanical loading plays an essential role in maintaining skeletal integrity. Mechanical stimulation leads to increased bone formation. However, the cellular and molecular mechanisms that are involved in the translation of mechanical stimuli into bone formation, are not completely understood. Growth factors and osteocytes, which act as mechanosensors, play a key role during the bone formation after mechanical stimulation. The aim of this study was to characterize the role of IGF-I in the translation of mechanical stimuli into bone formation locally in rat tibiae. Fifteen female Wistar rats were randomly assigned to three groups (n = 5): load, sham-loaded, and control. The four-point bending model of Forwood and Turner was used to induce a single period of mechanical loading on the tibia shaft. The effects of mechanical loading on IGF-I mRNA expression were determined with non-radioactive in situ hybridization on decalcified tibiae sections, 6 h after the loading session. Endogenous IGF-I mRNA was expressed in trabecular and cortical osteoblasts, some trabecular and sub-endocortical osteocytes, intracortical endothelial cells of blood vessels, and periosteum. Megakaryocytes, macrophages, and myeloid cells also expressed IGF-I mRNA. In the growth plate, IGF-I mRNA was located in proliferative and hypertrophic chondrocytes. Mechanical loading did not affect the IGF-I mRNA expression in osteoblasts, bone marrow cells, and chondrocytes, but the osteocytes at the endosteal side of the shaft showed a twofold increase of IGF-I mRNA expression. The proportion of IGF-I mRNA positive osteocytes in loaded tibiae was 29.3 ± 12.9% (mean ± s.d.; n = 5), whereas sham-loaded and contra-lateral control tibiae exhibited 16.7 ± 4.4% (n = 5) and 14.7 ± 4.2% (n = 10) respectively (P < 0.05). Lamellar bone formation after a single mechanical loading session was observed at the endosteal side of the shaft. In conclusion, a single loading session results in a twofold up-regulation of IGF-I mRNA synthesis in osteocytes which are present in multiple layers extending into the cortical bone of mechanically stimulated tibia shaft 6 h after loading. This supports the hypothesis that IGF-I, which is located in osteocytes, is involved in the translation of mechanical stimuli into bone formation.

scholarly journals Tissue concentration, mRNA expression and stimulation of IGF-I in luteal tissue during the oestrous cycle and pregnancy of cows

Reproduction ◽  
1990 ◽  
Vol 90 (2) ◽  
pp. 439-445 ◽  
Author(s):  
R. Einspanier ◽  
A. Miyamoto ◽  
D. Schams ◽  
M. Muller ◽  
G. Brem
Keyword(s):  
Mrna Expression ◽  
Tissue Concentration ◽  
Oestrous Cycle ◽  
Igf I ◽  
Luteal Tissue ◽  
Stimulation Of
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