Genetic diversity of avenin-like b genes in Aegilops tauschii Coss

Abstract Aegilops tauschii, the diploid progenitor of the wheat D-genome, is a valuable genetic resource for wheat breeders. In this study, we compared the efficiency of inter-simple sequence repeat (ISSR) (as an arbitrary technique) and start codon targeted (SCoT) (as a gene-targeting technique) markers in determining the genetic diversity and population structure of 90 accessions of Ae. tauschii. SCoT markers indicated the highest values for polymorphism information content, marker index and effective multiplex ratio compared to ISSR markers. The total genetic diversity (Ht) and genetic diversity within populations (Hs) parameters were comparably modest for the two marker systems. The results of the analysis of molecular variance showed that the genetic variation within populations was significantly higher than among them (ISSR: 92 versus 8%; SCoT: 88 versus 12%). Furthermore, SCoT markers discovered a high level of genetic differentiation among populations than ISSRs (0.19 versus 0.05), while the amount of gene flow detected by ISSR was higher than SCoT (2.13 versus 8.62). Cluster analysis and population structure of SCoT and ISSR data divided all investigated accessions into two and four main clusters, respectively. Our results revealed that SCoT and ISSR fingerprinting could be used to further molecular analysis in Ae. tauschii and other wild species. The high-genetic variability found in this study also indicates the valuable genetic potential present in the investigated Ae. tauschii germplasm, which could be utilized for future genetic analysis and linkage mapping in breeding programmes.

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Genetic diversity and gene-pool subdivisions of diploid D-genome Aegilops tauschii Coss. (Poaceae) in Iran as revealed by AFLP

Genetic Resources and Crop Evolution ◽

10.1007/s10722-008-9323-0 ◽

2008 ◽

Vol 55 (8) ◽

pp. 1231-1238 ◽

Cited By ~ 13

Author(s):

Hojjatollah Saeidi ◽

Badradin Ebrahim Sayed Tabatabaei ◽

Mehdi Rahimmalek ◽

Majid Talebi-Badaf ◽

Mohammad Reza Rahiminejad

Keyword(s):

Genetic Diversity ◽

Gene Pool ◽

Aegilops Tauschii ◽

D Genome

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Genetic diversity of Iranian Aegilops tauschii Coss. using microsatellite molecular markers and morphological traits

Molecular Biology Reports ◽

10.1007/s11033-009-9931-6 ◽

2009 ◽

Vol 37 (7) ◽

pp. 3413-3420 ◽

Cited By ~ 16

Author(s):

Zahra Tahernezhad ◽

Mohammad Javad Zamani ◽

Mahmood Solouki ◽

Mehdi Zahravi ◽

Abbas Ali Imamjomeh ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Markers ◽

Morphological Traits ◽

Aegilops Tauschii

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Evaluation of genetic diversity of Aegilops tauschii accessions using morphological characters

Journal of Crop Science and Biotechnology ◽

10.1007/s12892-013-0017-6 ◽

2013 ◽

Vol 16 (3) ◽

pp. 197-200 ◽

Cited By ~ 3

Author(s):

Sajjad Mansouri ◽

Ali Ashraf Mehrabi ◽

Danial Kahrizi

Keyword(s):

Genetic Diversity ◽

Aegilops Tauschii ◽

Morphological Characters

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ASSESSMENT OF THE GENETIC DIVERSITY IN AEGILOPS TAUSCHII (COSS.) BY USING SSR MARKERS AND MORPHYSIOLOGICAL TRAITS

Applied Ecology and Environmental Research ◽

10.15666/aeer/1805_70117020 ◽

2020 ◽

Vol 18 (5) ◽

pp. 7011-7020

Author(s):

A. ABBAS ◽

H.Y. YU ◽

H.L. CUI ◽

H.L. YU ◽

X.J. LI

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Aegilops Tauschii

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Efficient curation of genebanks using next generation sequencing reveals substantial duplication of germplasm accessions

10.1101/410779 ◽

2018 ◽

Cited By ~ 1

Author(s):

Narinder Singh ◽

Shuangye Wu ◽

W. John Raupp ◽

Sunish Sehgal ◽

Sanu Arora ◽

...

Keyword(s):

Genetic Diversity ◽

Next Generation Sequencing ◽

Crop Improvement ◽

Aegilops Tauschii ◽

Cost Effective ◽

Ex Situ ◽

Next Generation ◽

Sequencing Platform ◽

Efficient Management ◽

Generation Sequencing

ABSTRACTGenebanks are valuable resources for crop improvement through the acquisition, ex-situ conservation and sharing of unique germplasm among plant breeders and geneticists. With over seven million existing accessions and increasing storage demands and costs, genebanks need efficient characterization and curation to make them more accessible and usable and to reduce operating costs, so that the crop improvement community can most effectively leverage this vast resource of untapped novel genetic diversity. However, the sharing and inconsistent documentation of germplasm often results in unintentionally duplicated collections with poor characterization and many identical accessions that can be hard or impossible to identify without passport information and unmatched accession identifiers. Here we demonstrate the use of genotypic information from these accessions using a cost-effective next generation sequencing platform to find and remove duplications. We identify and characterize over 50% duplicated accessions both within and across genebank collections of Aegilops tauschii, an important wild relative of wheat and source of genetic diversity for wheat improvement. We present a pipeline to identify and remove identical accessions within and among genebanks and curate globally unique accessions. We also show how this approach can also be applied to future collection efforts to avoid the accumulation of identical material. When coordinated across global genebanks, this approach will ultimately allow for cost effective and efficient management of germplasm and better stewarding of these valuable resources.

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Distribution, Genetic Diversity and Population Structure of Aegilops tauschii Coss. in Major Wheat-Growing Regions in China

Agriculture ◽

10.3390/agriculture11040311 ◽

2021 ◽

Vol 11 (4) ◽

pp. 311

Author(s):

Haiyan Yu ◽

Juan Yang ◽

Hailan Cui ◽

Adeel Abbas ◽

Shouhui Wei ◽

...

Keyword(s):

Genetic Diversity ◽

Integrated Management ◽

Aegilops Tauschii ◽

Low Genetic Diversity ◽

Limited Gene Flow ◽

Different Populations ◽

New Perspective ◽

High Level ◽

Grass Weed ◽

Simple Sequence

Aegilops tauschii Coss. is known as a noxious grass weed seriously affecting wheat quality and yield. To investigate its present occurrence in wheat fields and the potential genetic diversity of the grass weed in China, a filed survey covering major wheat production regions was conducted during 2017–2019. Seeds of different Ae. tauschii populations collected from the survey were analyzed with Simple Sequence Repeats (SSRs) technique. Results showed that Ae. tauschii was occurring in each of the provinces surveyed with varied occurrence frequency ranging from 0.91% in Sichuan Province to 92.85% in Henan Provinces. Eighty alleles with size ranging from 98 bp to 277 bp were detected from the 192 collected Ae. tauschii populations with 17 SSR markers. Ae. tauschii, in this study, exhibited a moderately high level of genetic diversity, high differentiation, deficient heterozygosity and limited gene flow. Compared with other provinces, Hubei populations possessed relatively low genetic diversity. Dendrogram analysis showed that genetic distance did not seem to be related to geographic distribution. Additionally, STRUCTURE analysis suggested that Ae. tauschii populations in wheat fields of China can be divided into three groups, which was further supported by cluster analysis. Among the three groups, solely 7% of the total variation was detected, whereas the majority variation (67%) occurred among different populations within same group. Undoubtedly, such information will help us to better understand population relationships and spread of Ae. tauschii in China and will provide a new perspective for its integrated management.

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Evolution of the bread wheat D-subgenome and enriching it with diversity from Aegilops tauschii

10.1101/2021.01.31.428788 ◽

2021 ◽

Author(s):

Kumar Gaurav ◽

Sanu Arora ◽

Paula Silva ◽

Javier Sánchez-Martín ◽

Richard Horsnell ◽

...

Keyword(s):

Genetic Diversity ◽

Association Mapping ◽

Candidate Genes ◽

Bread Wheat ◽

Aegilops Tauschii ◽

Pest Resistance ◽

Wild Progenitor ◽

Genomic Regions ◽

Synthetic Hexaploids ◽

Diploid Ancestor

AbstractAegilops tauschii, the diploid wild progenitor of the D-subgenome of bread wheat, constitutes a reservoir of genetic diversity for improving bread wheat performance and environmental resilience. To better define and understand this diversity, we sequenced 242 Ae. tauschii accessions and compared them to the wheat D-subgenome. We characterized a rare, geographically-restricted lineage of Ae. tauschii and discovered that it contributed to the wheat D-subgenome, thereby elucidating the origin of bread wheat from at least two independent hybridizations. We then used k-mer-based association mapping to identify discrete genomic regions with candidate genes for disease and pest resistance and demonstrated their functional transfer into wheat by transgenesis and wide crossing, including the generation of a library of ‘synthetic’ hexaploids incorporating diverse Ae. tauschii genomes. This pipeline permits rapid trait discovery in the diploid ancestor through to functional genetic validation in a hexaploid background amenable to breeding.

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