Estimation of natural outcrossing rate and genetic diversity in Lima bean (Phaseolus lunatus L. var. lunatus) from Brazil using SSR markers: implications for conservation and breeding

2016 ◽  
Vol 64 (6) ◽  
pp. 1355-1364 ◽  
Author(s):  
J. S. Penha ◽  
A. C. A. Lopes ◽  
R. L. F. Gomes ◽  
J. B. Pinheiro ◽  
J. R. Assunção Filho ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Lima Bean ◽  
Outcrossing Rate ◽  
Phaseolus Lunatus

Genetic diversity in accessions of lima bean (Phaseolus lunatus L.) determined from agro-morphological descriptors and SSR markers for use in breeding programs in Brazil

2021 ◽  
Author(s):  
Carolline de Jesús Pires ◽  
Marcones Ferreira Costa ◽  
Maria Imaculada Zucchi ◽  
Regina Lucia Ferreira-Gomes ◽  
José Baldin Pinheiro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Morphological Descriptors ◽  
Breeding Programs

scholarly journals Genetic diversity analysis of Lima bean (Phaseolus lunatus L.) Landrace from Ethiopia as reveled by ISSR marker

2021 ◽  
Vol 44 (1) ◽  
pp. 81-90
Author(s):  
Leyila Nasir Leyila Nasir ◽  
Tileye Feyissa ◽  
Zemede Asfaw
Keyword(s):  
Genetic Diversity ◽  
Sustainable Use ◽  
Lima Bean ◽  
Issr Markers ◽  
Phaseolus Lunatus ◽  
Considerable Proportion ◽  
Improvement Strategy ◽  
Neglected Crops ◽  
Total Genetic Diversity ◽  
Planting Materials

Lima bean (Phaseolus lunatus L.) is one of the five most important legume crops from the genus Phaseolus though it is one of the neglected crops in Ethiopia. There is no national breeding program for its improvement. As it is true for all crops, understanding the genetic diversity of Lima bean is useful for its improvement and sustainable use. Therefore, the objective of the current study was to investigate genetic diversity of Lima bean landrace collected in Ethiopia using eight issr markers. A total of 96 landrace were collected from five administrative zones of Ethiopia from which 106 clear bands were detected. About 95 (88.7%) of the bands were polymorphic. The genetic diversity analyses result revealed that landrace collected from West Wellega showed the highest (0.1864) genetic diversity. amova demonstrated highly significant (P=0.00) genetic diversity among and within populations. A considerable proportion (66%) of the total genetic diversity was distributed among populations and 34% within populations. In addition, the calculated Fst value was high (Fst = 0.66), associated with a low gene flow value (Nm=0.27) indicating lower differentiation of the populations, which, in turn, implied no significant exchange of planting materials among farmers in the studied populations and the nature of the crops that Lima bean is 52% self pollinated crop. upgma, structure and PCoA analysis showed very strong grouping among individuals collected from the same zones and geographically distinct zones. Overall, genetic diversity achieved from this study could be used as pioneer information about the existing genetic resource for future Lima bean conservation and improvement strategy in Ethiopia.

scholarly journals Genetic diversity and promising crosses indication in lima bean (Phaseolus lunatus) accessions

2015 ◽  
Vol 36 (2) ◽  
pp. 683
Author(s):  
Verônica Brito da Silva ◽  
Regina Lucia Ferreira Gomes ◽  
Ângela Celis De Almeida Lopes ◽  
Carlos Tadeu Dos Santos Dias ◽  
Raimundo Nonato Oliveira Silva
Keyword(s):  
Genetic Diversity ◽  
Lima Bean ◽  
Phaseolus Lunatus

scholarly journals Differentiation and genetic diversity of Phaseolus lunatus wild populations from Chiapas, Mexico, and their genetic relationships with MI and MII groups

2016 ◽  
Vol 94 (4) ◽  
pp. 712
Author(s):  
Pedro Jesús Ruiz-Gil ◽  
Gabriel Chepe-Cruz ◽  
Rubén Humberto Andueza-Noh ◽  
Matilde Margarita Ortiz-García ◽  
Jaime Martínez-Castillo
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Genetic Divergence ◽  
Genetic Relationships ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Gene Pools ◽  
Wild Populations ◽  
Isthmus Of Tehuantepec ◽  
High Level

<p><strong>Background</strong><em>. </em>Understanding the genetic structure of wild relatives of domesticated species is crucial for its conservation and to elucidate the sites of crop domestication. Lima bean is one of the five domesticated <em>Phaseolus</em> species and Mexico is one of its centers of domestication. Recent studies showed the existence of two wild gene pools (MI and MII) of this species in Mexico and suggested that their genetic divergence occurred in southeast Mexico.</p><p><strong>Question</strong><em>. </em>Did MI and MII groups diverged around the Isthmus of Tehuantepec, Mexico?</p><p><strong>Studied species</strong><em>. </em>Lima bean: Neotropical plant species, herbaceous, with an annual/short life cycle and with autogamous tendency.</p><p><strong>Study site and years of study</strong><em>. </em>Seven wild populations were collected in 2012 in the State of Chiapas, México, area underrepresented in previous studies.</p><p><strong>Methods</strong><em>. </em>Genetic diversity and grouping patterns of collected populations and their relationship to MI and MII groups were analyzed at eight microsatellite loci.</p><p><strong>Results</strong>. High genetic structure (<em>F</em><sub>ST</sub>: 0.42 to 0.96) and a high level of genetic diversity (<em>H</em><sub>E </sub>= 0.48) were found. The analyses, and presence of admixed populations in MI and MII, suggested that the genetic divergence of these groups is an ongoing process centered around the Isthmus of Tehuantepec.</p><p><strong>Conclusions</strong><em>. </em>Our results support the hypothesis that MI and MII groups diverged around the Isthmus of Tehuantepec; however, sampling should be increased both at population and genomic levels, to determine the precise organization of the genetic diversity of wild <em>P. lunatus</em> from Mexico.</p>

Wild to crop introgression and genetic diversity in Lima bean (Phaseolus lunatus L.) in traditional Mayan milpas from Mexico

2014 ◽  
Vol 15 (6) ◽  
pp. 1315-1328 ◽  
Author(s):  
Dzul-Tejero Félix ◽  
Julián Coello-Coello ◽  
Jaime Martínez-Castillo
Keyword(s):  
Genetic Diversity ◽  
Lima Bean ◽  
Phaseolus Lunatus

Structure and Genetic Diversity of Wild Populations of Lima Bean (Phaseolus lunatus L.) from the Yucatan Peninsula, Mexico

Crop Science ◽  
2006 ◽  
Vol 46 (3) ◽  
pp. 1071-1080 ◽  
Author(s):  
Jaime Martínez-Castillo ◽  
Daniel Zizumbo-Villarreal ◽  
Paul Gepts ◽  
Patricia Delgado-Valerio ◽  
Patricia Colunga-GarcíaMarín
Keyword(s):  
Genetic Diversity ◽  
Yucatan Peninsula ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Wild Populations ◽  
Yucatán Peninsula

scholarly journals The Brazilian lima bean landraces: conservation and breeding

2021 ◽  
Author(s):  
Carolline de Jesús Pires ◽  
Marcones Ferreira Costa ◽  
Maria Imaculada Zucchi ◽  
Regina Lucia Ferreira-Gomes ◽  
José Baldin Pinheiro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Genetic Divergence ◽  
Polymorphic Information Content ◽  
Lima Bean ◽  
Optimization Method ◽  
Morphological Characterization ◽  
Phaseolus Lunatus ◽  
Lima Beans ◽  
On Farm

Abstract Brazil is one of the countries with the greatest genetic diversity in lima beans ( Phaseolus lunatus L.), which has been maintained both on farm and in germplasm banks. The knowledge of this diversity in the country is extremely important for developing a strategy for use and conservation. The objective of this study was characterizing landraces lima bean accessions from different regions in Brazil . Twenty two accessions conserved in the Phaseolus Germplasm Bank from UFPI (Piauí-Brazil) were characterized with 37 agro-morphological descriptors and 15 microsatellite markers. In the agro-morphological characterization, the maximum value of genetic divergence was obtained for the pair UFPI-262 and UFPI-252 (D = 88.74). The UPGMA grouping made it possible to form four groups. Tocher's optimization method enabled the formation of 10 groups. Regarding molecular characterization, 10 loci presented polymorphism, and the number of alleles per locus varied from two to seven. The Polymorphic Information Content (PIC) varied from 0.0767 to 0.7240. The loci GATS91 and PVat001 were highly informative and can be indicate for further studies involving the lima bean. The genetic diversity found (He = 0.316) was higher than that reported in the Yucatán Peninsula, a region indicated as a center of diversity for lima bean. Thus, the agro-morphological and molecular characterization were efficient in quantifying the genetic divergence between the studied accessions. The data found in this research provide a valuable resource for geneticists to subsidize breeding programs involving the lima bean.

scholarly journals Comparative analysis of Genetic Diversity among Pod Vegetables Genetic Resources Potential in Indonesia Revealed by ISSR Markers

2019 ◽  
Vol 10 (3) ◽  
pp. 161-172
Author(s):  
Ria Rif’atunidaudina ◽  
Sobir ◽  
Awang Maharijaya
Keyword(s):  
Genetic Diversity ◽  
Vigna Unguiculata ◽  
Genetic Relationships ◽  
Phenotypic Variability ◽  
Lima Bean ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Phaseolus Lunatus ◽  
Mucuna Pruriens ◽  
Velvet Bean

Cowpea (Vigna unguiculata ssp unguiculata), yardlong bean (Vigna unguiculata ssp sesquipedalis), Bambara groundnut (V.subterranea), lima bean (Phaseolus lunatus), bush bean (P. vulgaris), jack bean (Canavalia ensiformis), velvet bean (Mucuna pruriens) and winged bean (Psophocarpus tetragonolobus) are the important pod vegetable of the legume crop in Indonesia. These crops have a high economic and nutritional value. Its dry seeds are rich in proteins just like soybeans that can support human health and future food supply. The genetic diversity among different pod vegetables is not very well known. The objectives of this research were to determine the genetic relationships among different pod vegetable species based on ISSR markers. 32 accessions were analyzed by 11 ISSR primers. The result showed that the ISSR marker generated 80 DNA band with the polymorphism rate of 100% and the informative primers were PKBT 3 and PKBT 6. The result of cluster analysis and PCA analysis grouped all 32 accessions of the vegetable pod into eight clusters, indicating that the majority of the accession of a given species tend to group. Gower's similarity coefficient among all accessions varied from 0.425 to 0.988, and from 0.444 to 0.700 at the species level. The ISSR markers revealed the close relatedness between V. subterranea - C. ensiformis species, while the greatest distance was found between the P. vulgaris - M. pruriens species. Such a determination of relatedness is useful for a better understanding of the relationships among different pod vegetable species, which are generally considered to be a complex group with high phenotypic variability.   Keywords: clustering, genetic distance, polymorphism, pulses, similarity coefficient

scholarly journals GENETIC DIVERSITY OF LIMA BEAN (PHASEOLUS LUNATUS L.) FROM TIMOR ISLAND BASED ON MOLECULAR MARKER INTER-SIMPLE SEQUENCE REPEATS (ISSR)

Floribunda ◽  
2021 ◽  
Vol 6 (7) ◽  
Author(s):  
Emilia Juliyanti Bria ◽  
Polikarpia Wilhelmina Bani
Keyword(s):  
Genetic Diversity ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Breeding Programs ◽  
Pair Group ◽  
Inter Simple Sequence Repeats ◽  
Group Methods ◽  
Lima Beans ◽  
Issr Molecular Markers ◽  
Simple Sequence

Lima bean (Phaseolus lunatus L.) is one of the important legume vegetables in Indonesia. However, genetic information for these plants is still minimalized, especially on Timor Island. This study aims to analyze the genetic diversity of lima beans from Timor Island based on ISSR molecular markers. A total of 4 accessions of lima beans were analyzed using 3 ISSR primers to produce 15 polymorphic bands with an average of 68.18% polymorphism. The cluster analysis results use the Unweighted Pair Group Methods using Arithmetic averages (UPGMA) method to create a dendrogram that produces two main clusters. There were plain seed and pattern seed group with a similarity coefficient of 0.52. These results indicated that the genetic variation of the lima beans from Timor Island was high. Moreover, the result provides a sutable method for evaluating the genetic diversity of lima beans using the ISSR marker and important information of future lima bean breeding programs.

Genetic diversity and population structure of Populus yunnanensis revealed by SSR markers

2020 ◽  
Vol 52 (6) ◽  
Author(s):  
Anpei Zhou ◽  
Dan Zong ◽  
Peihua Gan ◽  
Yao Zhang ◽  
Dan Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers
Sign in / Sign up

Export Citation Format

Share Document