scholarly journals Comparative analysis of Genetic Diversity among Pod Vegetables Genetic Resources Potential in Indonesia Revealed by ISSR Markers

2019 ◽  
Vol 10 (3) ◽  
pp. 161-172
Author(s):  
Ria Rif’atunidaudina ◽  
Sobir ◽  
Awang Maharijaya
Keyword(s):  
Genetic Diversity ◽  
Vigna Unguiculata ◽  
Genetic Relationships ◽  
Phenotypic Variability ◽  
Lima Bean ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Phaseolus Lunatus ◽  
Mucuna Pruriens ◽  
Velvet Bean

Cowpea (Vigna unguiculata ssp unguiculata), yardlong bean (Vigna unguiculata ssp sesquipedalis), Bambara groundnut (V.subterranea), lima bean (Phaseolus lunatus), bush bean (P. vulgaris), jack bean (Canavalia ensiformis), velvet bean (Mucuna pruriens) and winged bean (Psophocarpus tetragonolobus) are the important pod vegetable of the legume crop in Indonesia. These crops have a high economic and nutritional value. Its dry seeds are rich in proteins just like soybeans that can support human health and future food supply. The genetic diversity among different pod vegetables is not very well known. The objectives of this research were to determine the genetic relationships among different pod vegetable species based on ISSR markers. 32 accessions were analyzed by 11 ISSR primers. The result showed that the ISSR marker generated 80 DNA band with the polymorphism rate of 100% and the informative primers were PKBT 3 and PKBT 6. The result of cluster analysis and PCA analysis grouped all 32 accessions of the vegetable pod into eight clusters, indicating that the majority of the accession of a given species tend to group. Gower's similarity coefficient among all accessions varied from 0.425 to 0.988, and from 0.444 to 0.700 at the species level. The ISSR markers revealed the close relatedness between V. subterranea - C. ensiformis species, while the greatest distance was found between the P. vulgaris - M. pruriens species. Such a determination of relatedness is useful for a better understanding of the relationships among different pod vegetable species, which are generally considered to be a complex group with high phenotypic variability.   Keywords: clustering, genetic distance, polymorphism, pulses, similarity coefficient

scholarly journals Genetic Diversity and Relationship of China’s Bergenia Germplasm Revealed by Intersimple Sequence Repeat Markers

2021 ◽  
pp. 1-7
Author(s):  
Xiuli Lv ◽  
Yuan Guan ◽  
Jian Wang ◽  
Yanwei Zhou ◽  
Qunlu Liu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Phylogenetic Relationship ◽  
Theoretical Basis ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Sequence Repeat ◽  
Relationship Of ◽  
Selection Of

To reveal the genetic diversity and genetic relationships of China’s Bergenia germplasm, 28 Bergenia accessions from different regions in China were analyzed by 24 intersimple sequence repeat (ISSR) markers. The results showed that 318 sites were amplified in all germplasm, including 307 polymorphic sites, and the percentage of polymorphic sites was 96.54%. Cluster analysis showed that the 28 accessions were divided into three categories, with a similarity coefficient of 0.5475. Bergenia purpurascens was clustered into one category; B. scopulosa was clustered into one category; and B. tianquaninsis, B. emeiensis, B. stracheyi, and B. crassifolia were clustered into one category. The results of the cluster analysis indicated that the 28 accessions were not completely classified by origin. Using the ISSR marker technique to analyze the phylogenetic relationship of Bergenia germplasm is helpful for identifying valuable resources and providing a theoretical basis for the selection of breeding parents.

Genetic diversity analysis in underutilized medicinal climber Mucuna pruriens (L.) DC. germplasm revealed by inter simple sequence repeats markers

2018 ◽  
Author(s):  
A. Chinapolaiah ◽  
K. Hima Bindu ◽  
G.N. Khadke ◽  
G.N. Manjesh ◽  
N. Hariprasad Rao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Issr Markers ◽  
Mucuna Pruriens ◽  
Diversity Analysis ◽  
Velvet Bean ◽  
Genetic Diversity Analysis ◽  
Inter Simple Sequence Repeats ◽  
Diversity Assessment ◽  
Simple Sequence

Velvet bean is an important medicinal legume, its seeds are prominent source of L-Dopa. The present investigation on genetic diversity assessment of 58 germplasm of velvet bean by using 11 ISSR markers. Out of 63 amplified products 59 were showed polymorphism and 4 were monomorphic with an average of 5.7 bands amplified per primer. According to band statistics and efficiency parameters showed the primers UBC 827, UBC 834 and UBC 836 were more efficient. The highest genetic similarity values (0.90) were observed between IIHR MP 102 and IIHR MP 74-3. In dendrogram germplasm grouped into two major clusters at 63 per cent similarity. Among the germplasm, IIHR Selection 4, IIHR Selection 10, IIHR MP 9, IC 33243 and IIHR MP 7 were found to be distinctly divergent, can be used in the further breeding programme.

scholarly journals Genetic diversity analysis of Lima bean (Phaseolus lunatus L.) Landrace from Ethiopia as reveled by ISSR marker

2021 ◽  
Vol 44 (1) ◽  
pp. 81-90
Author(s):  
Leyila Nasir Leyila Nasir ◽  
Tileye Feyissa ◽  
Zemede Asfaw
Keyword(s):  
Genetic Diversity ◽  
Sustainable Use ◽  
Lima Bean ◽  
Issr Markers ◽  
Phaseolus Lunatus ◽  
Considerable Proportion ◽  
Improvement Strategy ◽  
Neglected Crops ◽  
Total Genetic Diversity ◽  
Planting Materials

Lima bean (Phaseolus lunatus L.) is one of the five most important legume crops from the genus Phaseolus though it is one of the neglected crops in Ethiopia. There is no national breeding program for its improvement. As it is true for all crops, understanding the genetic diversity of Lima bean is useful for its improvement and sustainable use. Therefore, the objective of the current study was to investigate genetic diversity of Lima bean landrace collected in Ethiopia using eight issr markers. A total of 96 landrace were collected from five administrative zones of Ethiopia from which 106 clear bands were detected. About 95 (88.7%) of the bands were polymorphic. The genetic diversity analyses result revealed that landrace collected from West Wellega showed the highest (0.1864) genetic diversity. amova demonstrated highly significant (P=0.00) genetic diversity among and within populations. A considerable proportion (66%) of the total genetic diversity was distributed among populations and 34% within populations. In addition, the calculated Fst value was high (Fst = 0.66), associated with a low gene flow value (Nm=0.27) indicating lower differentiation of the populations, which, in turn, implied no significant exchange of planting materials among farmers in the studied populations and the nature of the crops that Lima bean is 52% self pollinated crop. upgma, structure and PCoA analysis showed very strong grouping among individuals collected from the same zones and geographically distinct zones. Overall, genetic diversity achieved from this study could be used as pioneer information about the existing genetic resource for future Lima bean conservation and improvement strategy in Ethiopia.

scholarly journals Differentiation and genetic diversity of Phaseolus lunatus wild populations from Chiapas, Mexico, and their genetic relationships with MI and MII groups

2016 ◽  
Vol 94 (4) ◽  
pp. 712
Author(s):  
Pedro Jesús Ruiz-Gil ◽  
Gabriel Chepe-Cruz ◽  
Rubén Humberto Andueza-Noh ◽  
Matilde Margarita Ortiz-García ◽  
Jaime Martínez-Castillo
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Genetic Divergence ◽  
Genetic Relationships ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Gene Pools ◽  
Wild Populations ◽  
Isthmus Of Tehuantepec ◽  
High Level

<p><strong>Background</strong><em>. </em>Understanding the genetic structure of wild relatives of domesticated species is crucial for its conservation and to elucidate the sites of crop domestication. Lima bean is one of the five domesticated <em>Phaseolus</em> species and Mexico is one of its centers of domestication. Recent studies showed the existence of two wild gene pools (MI and MII) of this species in Mexico and suggested that their genetic divergence occurred in southeast Mexico.</p><p><strong>Question</strong><em>. </em>Did MI and MII groups diverged around the Isthmus of Tehuantepec, Mexico?</p><p><strong>Studied species</strong><em>. </em>Lima bean: Neotropical plant species, herbaceous, with an annual/short life cycle and with autogamous tendency.</p><p><strong>Study site and years of study</strong><em>. </em>Seven wild populations were collected in 2012 in the State of Chiapas, México, area underrepresented in previous studies.</p><p><strong>Methods</strong><em>. </em>Genetic diversity and grouping patterns of collected populations and their relationship to MI and MII groups were analyzed at eight microsatellite loci.</p><p><strong>Results</strong>. High genetic structure (<em>F</em><sub>ST</sub>: 0.42 to 0.96) and a high level of genetic diversity (<em>H</em><sub>E </sub>= 0.48) were found. The analyses, and presence of admixed populations in MI and MII, suggested that the genetic divergence of these groups is an ongoing process centered around the Isthmus of Tehuantepec.</p><p><strong>Conclusions</strong><em>. </em>Our results support the hypothesis that MI and MII groups diverged around the Isthmus of Tehuantepec; however, sampling should be increased both at population and genomic levels, to determine the precise organization of the genetic diversity of wild <em>P. lunatus</em> from Mexico.</p>

Genetic relationships of Chinese prickly ash as revealed by ISSR markers

Biologia ◽  
2015 ◽  
Vol 70 (1) ◽  
Author(s):  
Shijing Feng ◽  
Tuxi Yang ◽  
Xiao Li ◽  
Lv Chen ◽  
Zhenshan Liu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationship ◽  
Genetic Divergence ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Chinese Prickly Ash ◽  
New Cultivars ◽  
Issr Primers

AbstractChinese prickly ash, belonging to the genus Zanthoxylum L., has been one of the most important commerciallyexploited plants for its alimentary, industrial and medicinal applications. However, the breeding and promotion of Chinese prickly ash have been severely restricted due to its confusing classification. Therefore, we assessed genetic diversity and phylogenetic relationship among 45 Chinese prickly ash samples collected from 6 main cultivated regions using 11 ISSR primers. These eleven selected primers generated a total of 102 scorable bands ranging from 150 to 2000 bp, corresponding to an average of approximately 9.3 bands per primer. The percentage of polymorphic loci for all samples ranged from 75% to 100%, with an average of 84.3%. The genetic similarity coefficient across all samples varied from 0.460 to 0.919. Remarkably, UPGMA analysis showed that 45 samples were divided into six clusters with a genetic similarity of 0.7. The closest genetic relationship was observed between Dahongpao collected from Qin’an and Tianshui, and the greatest genetic divergence was found between Dahongpao collected from Hengshui and Jiuyeqing collected from Jiangjin. It could serve as a basis for identifying Chinese prickly ash cultivars, breeding new cultivars and protecting the Chinese prickly ash resources in main regions.

Structural and some nutritional characteristics of Velvet bean (Mucuna pruriens) and Lima bean (Phaseolus lunatus) starches

2011 ◽  
Vol 63 (8) ◽  
pp. 475-484 ◽  
Author(s):  
David Betancur Ancona ◽  
Maira Rubi Segura Campos ◽  
Luis A. Chel Guerrero ◽  
Gloria Dávila Ortíz
Keyword(s):  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Mucuna Pruriens ◽  
Velvet Bean ◽  
Nutritional Characteristics

Genetic diversity in accessions of lima bean (Phaseolus lunatus L.) determined from agro-morphological descriptors and SSR markers for use in breeding programs in Brazil

2021 ◽  
Author(s):  
Carolline de Jesús Pires ◽  
Marcones Ferreira Costa ◽  
Maria Imaculada Zucchi ◽  
Regina Lucia Ferreira-Gomes ◽  
José Baldin Pinheiro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Morphological Descriptors ◽  
Breeding Programs

scholarly journals Analysis of genetic relationships of genotypes of the genus Rosa L. from the collection of Nikita Botanical Gardens using ISSR and IRAP DNA markers

2020 ◽  
Vol 24 (5) ◽  
pp. 474-480
Author(s):  
I. I. Suprun ◽  
S. A. Plugatar ◽  
I. V. Stepanov ◽  
T. S. Naumenko
Keyword(s):  
Genetic Diversity ◽  
Bayesian Methods ◽  
Dna Markers ◽  
Wild Species ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Issr Markers ◽  
The Other ◽  
Clustering Methods ◽  
Botanical Gardens

In connection with the development of breeding and the creation of new plant varieties, the problem of their genotyping and identification is becoming increasingly important, therefore the use of molecular methods to identify genetic originality and assess plant genetic diversity appears to be relevant. As part of the work performed, informative ISSR and IRAP DNA markers promising for the study of genetic diversity of the Rosa L. genus were sought and applied to analysis of genetic relationships among 26 accessions of the genus Rosa L. from the gene pool collection of Nikita Botanical Gardens. They included 18 cultivated varieties and 8 accessions of wild species. The species sample included representatives of two subgenera, Rosa and Platyrhodon. The subgenus Platyrhodon was represented by one accession of the species R. roxburghii Tratt. Cultivated roses were represented by varieties of garden groups hybrid tea, floribunda, and grandiflora. The tested markers included 32 ISSRs and 13 IRAPs. Five ISSR markers (UBC 824, ASSR29, 3A21, UBC 864, and UBC 843) and three IRAPs (TDK 2R, Сass1, and Сass2) were chosen as the most promising. They were used for genotyping the studied sample of genotypes. In general, they appeared to be suitable for further use in studying the genetic diversity of the genus Rosa L. The numbers of polymorphic fragments ranged from 12 to 31, averaging 19.25 fragments per marker. For markers UBC 864 and UBC 843, unique fingerprints were identified in each accession studied. The genetic relationships of the studied species and varieties of roses analyzed by the UPGMA, PCoA, and Bayesian methods performed on the basis of IRAP and ISSR genotyping are consistent with their taxonomic positions. The genotype of the species R. roxburghii of the subgenus Platyrhodon was determined genetically as the most distant. According to clustering methods, the representative of the species R. bengalensis did not stand out from the group of cultivated varieties. When assessing the level of genetic similarity among the cultivated varieties of garden roses, the most genetically isolated varieties were ‘Flamingo’, ‘Queen Elizabeth’, and ‘Kordes Sondermeldung’; for most of the other varieties, groups of the greatest genetic similarity were identified. This assessment reflects general trends in phylogenetic relationships, both among the studied species of the genus and among cultivated varieties.

scholarly journals The effect of heating technique to phytic acid content and antioxidant activity of velvet bean (Mucuna pruriens), butter bean (Phaseolus lunatus) and jack bean (Canavalia ensiformis)

2008 ◽  
Vol 6 (2) ◽  
pp. 36-44
Author(s):  
DIAN SRI PRAMITA ◽  
SRIiii HANDAJANI ◽  
DIAN RACHMAWANTI
Keyword(s):  
Antioxidant Activity ◽  
Phytic Acid ◽  
Acid Content ◽  
Variance Analysis ◽  
Phaseolus Lunatus ◽  
Mucuna Pruriens ◽  
Velvet Bean ◽  
Phytic Acid Content ◽  
Jack Bean ◽  
Canavalia Ensiformis

Pramita DS, Handajani S, Rachmawanti D. 2008. The effect of heating technique to phytic acid content and antioxidant activity of velvet bean (Mucuna pruriens), butter bean (Phaseolus lunatus) and jack bean (Canavalia ensiformis). Biofarmasi 6: 36-44. Koro is a kind of local bean which has many varieties. The nutrition of koro is not different with soy, especially carbohydrate and protein which high enough, and has a low-fat content. However, koro also contains some harmful compounds, HCN which poisoned and phytic acid which an antinutritional compound. Besides as antinutritional compound, phytic acid has a positive role, i.e. as an antioxidant. Besides phytic acid, legume also contains the compounds of phenol and vitamin E that have antioxidant activity. The aims of this research were to determine the contents of phytic acid and antioxidant activity, and to determine the effect of heating technique on phytic acid and antioxidant activity of velvet bean, butter bean, and jack bean. The materials used were velvet bean, butter bean and jack bean obtained from Batuwarno, Wonogiri, Central Java. This research used a Completely Randomized Design (CRD) with five kinds of treatment, each treatment consisted of three replications. The treatments given were soaking by 3 days (P1), steaming (P2), boiling (P3) and pressure cooker (P4), which compared to a raw bean without heating treatment (P0). The investigated factors were phytic acid and antioxidant activity (DPPH Radical Scavenging Ability method). The results of this research showed the phytic acid content of velvet bean, butter bean and jack bean from the treatment of P0, P1, P2, P3 and P4 were degraded. The phytic acid of velvet bean of P0, P1, P2, P3 and P4 treatment were 10.87, 8.94, 4.56 and 1.72 and 1.46 mg/db, respectively; on butter bean were 11.78, 8.75, 4.77, 1.73 and 1.61 mg/db, respectively; while on jack bean were 9.04, 1.99, 1.39, 1.42 and 1.21 mg/db. The result of variance analysis showed the phytic acid content was significantly different (p<0.05). Antioxidant activity showed the increase from P0 to P1, then the degradation process at P2, P3, and P4. Antioxidant activity at velvet bean were 74.10%, 86.49%, 84.73%, 83.59% and 79.51%, respectively; at butter bean were 4.5%, 7.19%, 6.07%, 6.30% and 6.28%, respectively; at jack bean were 14.64%, 8.55%, 5.84%, 5.17% and 3.58%. The result of variance analysis showed antioxidant activity at velvet bean and jack bean was significant, while at butter bean for P1, P2, P3, and P4 were not significant. The conclusion that could be taken away from this research were heating techniques had an effect on the degradation of phytic acid at all kind of bean used, and also had an effect on the antioxidant activity at velvet bean and jack bean.

scholarly journals  Genetic diversity of Czech apple cultivars inferred from microsatellite markers analysis

2012 ◽  
Vol 39 (No. 4) ◽  
pp. 149-157 ◽  
Author(s):  
J. Patzak ◽  
F. Paprštein ◽  
A. Henychová ◽  
J. Sedlák
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Simple Sequence Repeat ◽  
Cultivar Identification ◽  
Genetic Relationships ◽  
Similarity Coefficient ◽  
Collection Management ◽  
Apple Cultivars ◽  
Repeat Primer ◽  
Simple Sequence

Genetic diversity and genetic relationships of Czech apple cultivars were evaluated. Trees of 33&nbsp;Czech apple cultivars and 97 reference foreign cultivars were analysed using the set of 10 SSR (simple sequence repeat) primer pairs. The total of 89 polymorphic alleles were amplified, while the number of alleles per locus ranged from 4 to 14. The SSR dendrogram, based on the Jaccard&rsquo;s similarity coefficient, divided apple cultivars into three major groups: Cox&rsquo;s Orange Pippin, McIntosh and Golden Delicious ancestries. The clustering highly depended on pedigree and origin of apple cultivars. Spontaneous mutated cultivars were identical with their progenitors. We proved that microsatellite markers were useful for evaluation of genetic resources, collection management and cultivar identification. &nbsp;

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