scholarly journals Genetic diversity analysis of Lima bean (Phaseolus lunatus L.) Landrace from Ethiopia as reveled by ISSR marker

2021 ◽  
Vol 44 (1) ◽  
pp. 81-90
Author(s):  
Leyila Nasir Leyila Nasir ◽  
Tileye Feyissa ◽  
Zemede Asfaw
Keyword(s):  
Genetic Diversity ◽  
Sustainable Use ◽  
Lima Bean ◽  
Issr Markers ◽  
Phaseolus Lunatus ◽  
Considerable Proportion ◽  
Improvement Strategy ◽  
Neglected Crops ◽  
Total Genetic Diversity ◽  
Planting Materials

Lima bean (Phaseolus lunatus L.) is one of the five most important legume crops from the genus Phaseolus though it is one of the neglected crops in Ethiopia. There is no national breeding program for its improvement. As it is true for all crops, understanding the genetic diversity of Lima bean is useful for its improvement and sustainable use. Therefore, the objective of the current study was to investigate genetic diversity of Lima bean landrace collected in Ethiopia using eight issr markers. A total of 96 landrace were collected from five administrative zones of Ethiopia from which 106 clear bands were detected. About 95 (88.7%) of the bands were polymorphic. The genetic diversity analyses result revealed that landrace collected from West Wellega showed the highest (0.1864) genetic diversity. amova demonstrated highly significant (P=0.00) genetic diversity among and within populations. A considerable proportion (66%) of the total genetic diversity was distributed among populations and 34% within populations. In addition, the calculated Fst value was high (Fst = 0.66), associated with a low gene flow value (Nm=0.27) indicating lower differentiation of the populations, which, in turn, implied no significant exchange of planting materials among farmers in the studied populations and the nature of the crops that Lima bean is 52% self pollinated crop. upgma, structure and PCoA analysis showed very strong grouping among individuals collected from the same zones and geographically distinct zones. Overall, genetic diversity achieved from this study could be used as pioneer information about the existing genetic resource for future Lima bean conservation and improvement strategy in Ethiopia.

scholarly journals Comparative analysis of Genetic Diversity among Pod Vegetables Genetic Resources Potential in Indonesia Revealed by ISSR Markers

2019 ◽  
Vol 10 (3) ◽  
pp. 161-172
Author(s):  
Ria Rif’atunidaudina ◽  
Sobir ◽  
Awang Maharijaya
Keyword(s):  
Genetic Diversity ◽  
Vigna Unguiculata ◽  
Genetic Relationships ◽  
Phenotypic Variability ◽  
Lima Bean ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Phaseolus Lunatus ◽  
Mucuna Pruriens ◽  
Velvet Bean

Cowpea (Vigna unguiculata ssp unguiculata), yardlong bean (Vigna unguiculata ssp sesquipedalis), Bambara groundnut (V.subterranea), lima bean (Phaseolus lunatus), bush bean (P. vulgaris), jack bean (Canavalia ensiformis), velvet bean (Mucuna pruriens) and winged bean (Psophocarpus tetragonolobus) are the important pod vegetable of the legume crop in Indonesia. These crops have a high economic and nutritional value. Its dry seeds are rich in proteins just like soybeans that can support human health and future food supply. The genetic diversity among different pod vegetables is not very well known. The objectives of this research were to determine the genetic relationships among different pod vegetable species based on ISSR markers. 32 accessions were analyzed by 11 ISSR primers. The result showed that the ISSR marker generated 80 DNA band with the polymorphism rate of 100% and the informative primers were PKBT 3 and PKBT 6. The result of cluster analysis and PCA analysis grouped all 32 accessions of the vegetable pod into eight clusters, indicating that the majority of the accession of a given species tend to group. Gower's similarity coefficient among all accessions varied from 0.425 to 0.988, and from 0.444 to 0.700 at the species level. The ISSR markers revealed the close relatedness between V. subterranea - C. ensiformis species, while the greatest distance was found between the P. vulgaris - M. pruriens species. Such a determination of relatedness is useful for a better understanding of the relationships among different pod vegetable species, which are generally considered to be a complex group with high phenotypic variability.   Keywords: clustering, genetic distance, polymorphism, pulses, similarity coefficient

Genetic diversity in accessions of lima bean (Phaseolus lunatus L.) determined from agro-morphological descriptors and SSR markers for use in breeding programs in Brazil

2021 ◽  
Author(s):  
Carolline de Jesús Pires ◽  
Marcones Ferreira Costa ◽  
Maria Imaculada Zucchi ◽  
Regina Lucia Ferreira-Gomes ◽  
José Baldin Pinheiro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Morphological Descriptors ◽  
Breeding Programs

scholarly journals Structure and Genetic Diversity of Nine Important Landraces of Capsicum Species Cultivated in the Yucatan Peninsula, Mexico

Agronomy ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 376 ◽  
Author(s):  
Lucero del C. López Castilla ◽  
René Garruña Hernández ◽  
Crescencio de la Cruz Castillo Aguilar ◽  
Aida Martínez-Hernández ◽  
Matilde Margarita Ortiz-García ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Yucatan Peninsula ◽  
Conservation Status ◽  
Sustainable Use ◽  
Issr Markers ◽  
Southern Mexico ◽  
Yucatán Peninsula ◽  
Capsicum Spp ◽  
Capsicum Species

Mexico has a wealth of Capsicum species, which has led to the development of a large number of chili pepper landraces. A great wealth of Capsicum germplasm can be found in southern Mexico in the Yucatan Peninsula, an important area of diversification of Capsicum annuum. Specifically, in the western Yucatan Peninsula, three of the five domesticated species of Capsicum (C. annuum, C. chinense and C. frutescens) have been reported. However, information on their genetic diversity, conservation status and potential use is lacking. To generate useful information toward the sustainable use, management and conservation of these species, we evaluated the structure, diversity and genetic relationships of nine accessions of Capsicum spp., of major importance cultivated in the western Yucatan Peninsula using 42 ISSR loci. The results indicated that these accessions consisted of three genetic groups that were defined by the respective species of each accession. The level of genetic diversity was moderate and distributed mainly among accessions. The ISSR markers detected a high level of polymorphism and allowed the genetic differentiation of the C. annuum complex. The results indicated that the accessions collected in the western Yucatan Peninsula constitute a valuable genetic resource that can be used in genetic improvement and conservation programs.

scholarly journals Genetic diversity and promising crosses indication in lima bean (Phaseolus lunatus) accessions

2015 ◽  
Vol 36 (2) ◽  
pp. 683
Author(s):  
Verônica Brito da Silva ◽  
Regina Lucia Ferreira Gomes ◽  
Ângela Celis De Almeida Lopes ◽  
Carlos Tadeu Dos Santos Dias ◽  
Raimundo Nonato Oliveira Silva
Keyword(s):  
Genetic Diversity ◽  
Lima Bean ◽  
Phaseolus Lunatus

scholarly journals Differentiation and genetic diversity of Phaseolus lunatus wild populations from Chiapas, Mexico, and their genetic relationships with MI and MII groups

2016 ◽  
Vol 94 (4) ◽  
pp. 712
Author(s):  
Pedro Jesús Ruiz-Gil ◽  
Gabriel Chepe-Cruz ◽  
Rubén Humberto Andueza-Noh ◽  
Matilde Margarita Ortiz-García ◽  
Jaime Martínez-Castillo
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Genetic Divergence ◽  
Genetic Relationships ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Gene Pools ◽  
Wild Populations ◽  
Isthmus Of Tehuantepec ◽  
High Level

<p><strong>Background</strong><em>. </em>Understanding the genetic structure of wild relatives of domesticated species is crucial for its conservation and to elucidate the sites of crop domestication. Lima bean is one of the five domesticated <em>Phaseolus</em> species and Mexico is one of its centers of domestication. Recent studies showed the existence of two wild gene pools (MI and MII) of this species in Mexico and suggested that their genetic divergence occurred in southeast Mexico.</p><p><strong>Question</strong><em>. </em>Did MI and MII groups diverged around the Isthmus of Tehuantepec, Mexico?</p><p><strong>Studied species</strong><em>. </em>Lima bean: Neotropical plant species, herbaceous, with an annual/short life cycle and with autogamous tendency.</p><p><strong>Study site and years of study</strong><em>. </em>Seven wild populations were collected in 2012 in the State of Chiapas, México, area underrepresented in previous studies.</p><p><strong>Methods</strong><em>. </em>Genetic diversity and grouping patterns of collected populations and their relationship to MI and MII groups were analyzed at eight microsatellite loci.</p><p><strong>Results</strong>. High genetic structure (<em>F</em><sub>ST</sub>: 0.42 to 0.96) and a high level of genetic diversity (<em>H</em><sub>E </sub>= 0.48) were found. The analyses, and presence of admixed populations in MI and MII, suggested that the genetic divergence of these groups is an ongoing process centered around the Isthmus of Tehuantepec.</p><p><strong>Conclusions</strong><em>. </em>Our results support the hypothesis that MI and MII groups diverged around the Isthmus of Tehuantepec; however, sampling should be increased both at population and genomic levels, to determine the precise organization of the genetic diversity of wild <em>P. lunatus</em> from Mexico.</p>

scholarly journals Genetic diversity and phenetic relationships of five Trifolium L. species (Fabaceae) by inter simple sequence repeats markers

2016 ◽  
Vol 23 (2) ◽  
pp. 167-173
Author(s):  
Yourang Hwang ◽  
Man Kyu Huh
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Gene Flow ◽  
Simple Sequence Repeats ◽  
Issr Markers ◽  
Phenetic Relationships ◽  
Inter Simple Sequence Repeats ◽  
Total Genetic Diversity ◽  
Plant Taxon ◽  
Simple Sequence

Five species of Trifolium L. (T. repens L., T. pretense L., T. hybridum L., T. campestre Schreb., and T. dubium Sibth.) were analyzed used to evaluate the genetic diversity and their phenetic relationships using inter-simple sequence repeats (ISSR) markers. Overall, T. pratense exhibited higher variation than other species. 114 amplicons were produced by ISSR markers, of which 77 (67.5%) bands were polymorphic. T. dubium showed the low genetic variation. Total genetic diversity values (HT) varied between 0.333 and 0.487, for an average over all polymorphic loci of 0.282. On a perlocus basis, the proportion of total genetic variation due to differences among species (GST) was 0.380. This indicated that about 38.0% of the total variation was among species. The estimate of gene flow, based on GST, was very low among species of genus Trifolium (Nm = 0.816). An assessment of the proportion of diversity present within species, HPOP/HSP, indicated that about 95.8% the total genetic diversity was within species. T. pratense and T. hybridum were grouped together and this clade was sister with T. repens. Two remainder species with yellow flowers were grouped together. Information on genetic diversity for Trifolium is valued for the management of germplasm and for evolving conservation strategies.Bangladesh J. Plant Taxon. 23(2): 167-173, 2016 (December)

Wild to crop introgression and genetic diversity in Lima bean (Phaseolus lunatus L.) in traditional Mayan milpas from Mexico

2014 ◽  
Vol 15 (6) ◽  
pp. 1315-1328 ◽  
Author(s):  
Dzul-Tejero Félix ◽  
Julián Coello-Coello ◽  
Jaime Martínez-Castillo
Keyword(s):  
Genetic Diversity ◽  
Lima Bean ◽  
Phaseolus Lunatus

Structure and Genetic Diversity of Wild Populations of Lima Bean (Phaseolus lunatus L.) from the Yucatan Peninsula, Mexico

Crop Science ◽  
2006 ◽  
Vol 46 (3) ◽  
pp. 1071-1080 ◽  
Author(s):  
Jaime Martínez-Castillo ◽  
Daniel Zizumbo-Villarreal ◽  
Paul Gepts ◽  
Patricia Delgado-Valerio ◽  
Patricia Colunga-GarcíaMarín
Keyword(s):  
Genetic Diversity ◽  
Yucatan Peninsula ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Wild Populations ◽  
Yucatán Peninsula

scholarly journals Study of genetic diversity in Tunisian local cattle populations using ISSR markers

2019 ◽  
Vol 42.3 ◽  
pp. 7296-7302
Author(s):  
Haifa EL HENTATI ◽  
Najia THAMRI ◽  
Wissal DEROUICH ◽  
Mouna HADHLI ◽  
Taoufik BOUKHORSA
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Issr Markers ◽  
Genetic Distances ◽  
Sequence Repeat ◽  
North West ◽  
Upgma Dendrogram ◽  
The North ◽  
Total Genetic Diversity ◽  
Issr Primers

The inter-simple DNA sequence repeat (ISSR) method was used to study genetic diversity in three local cattle from the north, northeast and north west of Tunisia. Twenty samples were analysed using three ISSR primers. In total, 22 bands were amplified of which 15 are polymorphic (68.18%). The total genetic diversity (Ht), genetic diversity within populations (Hs), coefficient of gene differentiation (Gst) and gene flow (Nm) were 0.2706, 0.01314, 0.8841 and 0.0656. To better visualize the structure of the population, a UPGMA dendrogram constructed from the genetic distances of NEI shows that the populations of North (Bizerte) and Northeast (Nabeul) are genetically closest while that of Northwest (Jendouba and Siliana) is the furthest from the two others.

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