Effect of alkaline pH and nitrogen starvation on the triacylglycerol (TAG) content, growth, biochemical composition, and fatty acid profile of Auxenochlorella protothecoides KP7

Author(s):  
Enver Ersoy Andeden ◽  
Sahlan Ozturk ◽  
Belma Aslim
Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3582 ◽  
Author(s):  
Izabela Krzemińska ◽  
Marta Oleszek ◽  
Dariusz Wiącek

In recent years, there has been growing interest in the biomass of unicellular algae as a source of valuable metabolites. The main limitations in the commercial application of microbial biomass are associated with the costs of production thereof. Maize silage is one of the main substrates used in biogas plants in Europe. The effects of sterilized agricultural liquid digestate (LD) from methane fermentation of maize silage on the growth rates, macro and micronutrient removal efficiency, lipid content, and fatty acid profile in Auxenochlorella protothecoides were investigated. The results indicate that A. prothecoides can proliferate and accumulate lipids with simultaneous reduction of nutrients in the 1:20 diluted liquid digestate. The rate of nitrogen and phosphorus removal from the liquid digestate was 79.45% and 78.4%, respectively. Cells growing in diluted liquid digestate exhibited the maximum lipid content, i.e., 44.65%. The fatty acid profile of A. prothecoides shows a decrease in the content of linolenic acid by 20.87% and an increase in oleic acid by 32.16% in the LD, compared with the control. The liquid digestate changed the content of monounsaturated fatty acids and polyunsaturated fatty acids. The cells of A. protothecoides growing in the liquid digestate were characterized by lower PUFA content and higher MUFA levels.


2017 ◽  
Vol 69 (3) ◽  
pp. 491-501 ◽  
Author(s):  
Mayra González-Félix ◽  
Perla Urquidez-Bejarano ◽  
Martin Perez-Velazquez ◽  
Reina Castro-Longoria ◽  
Celia Vazquez-Boucard

Cynoscion parvipinnis is an important fisheries resource of the Gulf of California used locally for human consumption. Its aquaculture potential has recently been demonstrated, and cultured organisms are already available. In this study, the gonadal developmental stage, proximate composition, fatty acid profile and other biochemical and biological indices of wild and cultured fish were characterized. A total of 80 fish, 30 cultured (15 female and 15 male) and 50 wild (31 female and 19 male), captured in November at Santa Rosa, Sonora, Mexico, were analyzed. Histological analyses of the gonads showed that 100% of the wild and cultured fish were at an early maturation stage. Wild fish had greater body weight, total length and gonadosomatic index. Cultured female fish (19.64%) possessed a slightly higher protein content in their gonads than wild females (17.11%), whereas males had similar values (14.00 and 14.83%). Cultured females (10.85%) and males (20.48%) had a significantly greater crude fat content than wild fish (female: 2.05%, male: 11.05%). Palmitic acid was the most abundant fatty acid (FA), ranging from 128.58-164.84 mg of 16:0 g-1 of gonad wet weight. After 16:0, highly unsaturated FAs like DHA, ARA and EPA were the major FA in gonadal tissue; n-3 FAs were quantitatively higher than n-6 FAs, while the n-3/n-6 ratios ranged from 2.08-2.81. In general, the biochemical composition of the gonad of wild and cultured organisms at an early maturation stage was quite similar. These data may serve as indicators of dietary requirements for a maturation diet for broodstock culture.


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