Highly Sensitive Immunofluorescence Assay of Prostate-Specific Antigen Using Silver Nanoparticles

2020 ◽  
Vol 87 (5) ◽  
pp. 870-876
Author(s):  
I. V. Koktysh ◽  
Ya. I. Melnikova ◽  
O. S. Kulakovich ◽  
A. A. Ramanenka ◽  
S. V. Vaschenko ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Immunofluorescence Assay ◽  
Highly Sensitive

Highly sensitive nanoparticle-based immunoassays with elemental detection: Application to Prostate-Specific Antigen quantification

2016 ◽  
Vol 85 ◽  
pp. 128-134 ◽  
Author(s):  
Marta Garcia-Cortes ◽  
Jorge Ruiz Encinar ◽  
Jose M. Costa-Fernandez ◽  
Alfredo Sanz-Medel
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Highly Sensitive ◽  
Elemental Detection

Ultrasensitive photoelectrochemical immunoassay for prostate-specific antigen based on silver nanoparticles-triggered ion-exchange reaction with ZnO/CdS nanorods

The Analyst ◽  
2021 ◽  
Author(s):  
Ling Zhu ◽  
Zipeng Yin ◽  
Zijian Lv ◽  
Mei-Jin Li ◽  
Dianping Tang
Keyword(s):  
Prostate Cancer ◽  
Silver Nanoparticles ◽  
Ion Exchange ◽  
Exchange Reaction ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Cds Nanorods ◽  
Ion Exchange Reaction ◽  
Photoelectrochemical Immunoassay

Prostate-specific antigen (PSA), a glycoprotein that is most likely to cause prostate cancer, has attracted widespread attention in recent years due to its increasing threat to people's life and health....

Prostate-specific antigen in milk of lactating women

1995 ◽  
Vol 41 (1) ◽  
pp. 54-58 ◽  
Author(s):  
H Yu ◽  
E P Diamandis
Keyword(s):  
Breast Milk ◽  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Normal Breast ◽  
Specific Marker ◽  
Time Resolved ◽  
Lactating Women ◽  
Highly Sensitive ◽  
Mother’S Age ◽  
Total Psa

Abstract Prostate-specific antigen (PSA) is believed to be a highly specific marker for normal and cancerous prostatic tissue. We recently found that 30-40% of breast tumors produce PSA. Other data from our group suggest that normal breast can also produce PSA under conditions of stimulation by steroid hormones. In addition, we detected PSA in amniotic fluid. Here we report the presence of PSA in breast milk of lactating women. PSA concentrations in breast milk were quite variable, ranging from < 0.01 microgram/L in 4 of 38 milks to 350 micrograms/L; the median was 0.47 microgram/L. PSA concentration in breast milk was not correlated with mother's age or the sex of the newborn. It did tend to decrease with increasing time postdelivery, but was still detectable 2 weeks postdelivery. PSA in milk was equally measurable by a highly sensitive PSA assay based on time-resolved fluorometry and by the IMx automated PSA method. As confirmed by Western blot analysis, PSA in milk was present predominantly in its 33-kDa form; the PSA-alpha 1-antichymotrypsin complex (100 kDa) was also present but its concentration was < 25% of total PSA. We conclude that the female breast can produce PSA and that PSA is secreted into the milk during lactation; however, the biological role of PSA in milk is unknown. These and other data presented by our group suggest that PSA, a serine protease, may play a role in control of growth in mammary and other tissues through regulation of growth factors, cytokines, and growth-factor-binding proteins.

scholarly journals Rational design of Au nanorods assemblies for highly sensitive and selective SERS detection of prostate specific antigen

RSC Advances ◽  
2015 ◽  
Vol 5 (48) ◽  
pp. 38354-38360 ◽  
Author(s):  
An-qi Yang ◽  
Dong Wang ◽  
Xiang Wang ◽  
Yu Han ◽  
Xue-bin Ke ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Rapid Detection ◽  
Rational Design ◽  
Specific Antigen ◽  
Early Diagnostics ◽  
Au Nanorods ◽  
Highly Sensitive ◽  
Sers Detection

A simple SERS immunosensor based on AuNRs assembly was developed for rapid detection of specific antigen in early diagnostics.

Prostate-Specific Antigen Levels from a Mass Screening Program Using Highly Sensitive RIA Kits

1997 ◽  
Vol 4 (3) ◽  
pp. 269-273
Author(s):  
Susumu Akimoto ◽  
Tomohiko Ichikawa ◽  
Koichiro Akakura ◽  
Jun Shimazaki
Keyword(s):  
Prostate Specific Antigen ◽  
Mass Screening ◽  
Screening Program ◽  
Specific Antigen ◽  
Highly Sensitive

Activated phosphonated trifunctional chelates for highly sensitive lanthanide-based FRET immunoassays applied to total prostate specific antigen detection

2013 ◽  
Vol 11 (38) ◽  
pp. 6493 ◽  
Author(s):  
Katia Nchimi-Nono ◽  
K. David Wegner ◽  
Stina Lindén ◽  
Alexandre Lecointre ◽  
Laurence Ehret-Sabatier ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Antigen Detection ◽  
Total Prostate Specific Antigen ◽  
Highly Sensitive

Serum prostate-specific antigen measured in children from birth to age 18 years

1996 ◽  
Vol 42 (3) ◽  
pp. 420-423 ◽  
Author(s):  
E W Randell ◽  
E P Diamandis ◽  
G Ellis
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Ambiguous Genitalia ◽  
Male Gender ◽  
Blood Samples ◽  
Male And Female ◽  
Highly Sensitive ◽  
Males And Females ◽  
Cross Matching ◽  
Stimulation Of

Abstract We measured prostate-specific antigen (PSA) in serum from 94 cord- blood samples, from 44 newborns, and from 330 children up to age 18 years, using a highly sensitive "third generation" PSA assay on the IMMULITE (Diagnostic Products Corp.) analyzer. The serum was that remaining after cross-matching for blood transfusion. Most children were hospitalized for special care or surgery. We found detectable concentrations of PSA (> or = 0.003 micrograms/L) in many cord sera and in sera from both male and female neonates. PSA was more frequently detectable in cord and newborn sera from males than from females, but there was considerable overlap in values between the sexes, negating any possible usefulness of PSA for assigning male gender to newborns with ambiguous genitalia. PSA decreased to undetectable concentrations in most prepubertal males and females but became detectable around the age of puberty in males. We speculate that the presence of detectable PSA in cord and newborn sera results from androgenic stimulation of prostatic tissue in males or from stimulation of breast or other tissue by prolactin or progesterone in females.

scholarly journals Detection of prostate-specific antigen mRNA and protein in breast tumors

1996 ◽  
Vol 42 (3) ◽  
pp. 361-366 ◽  
Author(s):  
N Zarghami ◽  
E P Diamandis
Keyword(s):  
Polymerase Chain Reaction ◽  
Prostate Specific Antigen ◽  
Reverse Transcription ◽  
Specific Antigen ◽  
Breast Tumors ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Polymerase Chain ◽  
Good Agreement

Abstract We have developed reverse transcription-polymerase chain reaction (RT- PCR) methods for detecting prostate-specific antigen (PSA) mRNA. Using these methods, and a highly sensitive immunofluorometric assay for measuring PSA protein, we have assessed the concentrations of PSA mRNA and PSA protein in 30 primary breast tumors and a few other control tissues. We found good agreement between presence of PSA protein and PSA mRNA in breast tumors. We thus propose that, in women, detection of PSA protein or PSA mRNA in tissues and tumors offers equivalent information. Because PSA protein is present in male blood and thus could contaminate extracts from tumors and tissues from men, we propose that the RT-PCR methods we describe be used to assess nonprostatic expression of the PSA gene in men.

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