Highly sensitive spectrofluorimetric determination of lomefloxacin in spiked human plasma, urine and pharmaceutical preparations

2009 ◽  
Vol 44 (9) ◽  
pp. 3402-3405 ◽  
Author(s):  
Sevgi Tatar Ulu
Keyword(s):  
Human Plasma ◽  
Pharmaceutical Preparations ◽  
Spiked Human Plasma ◽  
Highly Sensitive ◽  
Spectrofluorimetric Determination

scholarly journals Second-Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Cinnarizine and Nicergoline in Pharmaceutical Preparations

2008 ◽  
Vol 91 (2) ◽  
pp. 349-359 ◽  
Author(s):  
Mohamed I Walash ◽  
Fathalla Belal ◽  
Nahed El-Enany ◽  
Amina Abdelal
Keyword(s):  
Human Plasma ◽  
Pharmaceutical Preparations ◽  
High Sensitivity ◽  
Second Derivative ◽  
Native Fluorescence ◽  
Spiked Human Plasma ◽  
Highly Sensitive ◽  
Experimental Parameters ◽  
Good Agreement

Abstract A rapid, simple, and highly sensitive second-derivative synchronous fluorometric method has been developed for the simultaneous analysis of binary mixtures of cinnarizine (CN) and nicergoline (NIC). The method is based upon measurement of the native fluorescence of these drugs at constant wavelength difference () = 80 nm in aqueous methanol (50, v/v). The different experimental parameters affecting the native fluorescence of the studied drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the range of 0.0251.5 and 0.255.5 g/mL for CN and NIC, respectively, with lower detection limits of 0.58 and 0.82 ng/mL and quantitation limits of 1.93 and 2.73 ng/mL for CN and NIC, respectively. The proposed method was successfully applied for the determination of the studied compounds in synthetic mixtures and in commercial tablets. The results obtained were in good agreement with those obtained with reference methods. The high sensitivity attained by the proposed method allowed the determination of CN in real and spiked human plasma. The mean recovery in the case of spiked human plasma [number of trials (n) = 3] was 102.82 2.17, while that in real human plasma (n = 3) was 105.25 2.05.

scholarly journals Spectrofluorometric Determination of Verapamil Hydrochloride in Pharmaceutical Preparations and Human Plasma Using Organized Media: Application to Stability Studies

2006 ◽  
Vol 89 (6) ◽  
pp. 1565-1572 ◽  
Author(s):  
Mohamed Walash ◽  
Fathalla Belal ◽  
Nahed El-Enany ◽  
Amina Abdelsalam
Keyword(s):  
Human Plasma ◽  
Biological Fluids ◽  
Sodium Dodecyl ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Official Method ◽  
Stability Studies ◽  
Verapamil Hydrochloride ◽  
Spiked Human Plasma

Abstract A highly sensitive spectrofluorometric method was developed for the determination of verapamil hydrochloride (VP HCl) in pharmaceutical formulations and biological fluids. The proposed method is based on investigation of the fluorescence spectral behavior of VP HCl in micellar systems, such as sodium dodecyl sulfate (SDS) and β-cyclodextrin (β-CD). In aqueous solutions of borate buffer of pH 9 and 8.5, VP HCl was well incorporated into SDS and β-CD, respectively, with enhancement of its native fluorescence. The fluorescence was measured at 318 nm after excitation at 231 nm. The fluorescence intensity enhancements were 183 and 107% in SDS and in β-CD, respectively. The fluorescence-concentration plots were rectilinear over the range of 0.020.2 and 0.020.25 μg/mL, with lower detection limits of 5.58 × 103 and 3.62 × 103 μg/mL in SDS and β-CD, respectively. The method was successfully applied to the analysis of commercial tablets and the results were in good agreement with those obtained with the official method. The method was further applied to the determination of VP HCl in real and spiked human plasma. The mean % recoveries in the case of spiked human plasma (n 4) was 92.59 3.11 and 88.35 2.55 using SDS and β-CD, respectively, while that in real human plasma (n 3) was 90.17 6.93 and 89.17 6.50 using SDS and β-CD, respectively. The application of the method was extended to the stability studies of VP HCl after exposureto ultraviolet radiation and upon oxidation with hydrogen peroxide.

scholarly journals Spectrofluorimetric Determination of Warfarin Sodium by Using N1-Methylnicotinamide Chloride as a Fluorigenic Agent

2005 ◽  
Vol 88 (2) ◽  
pp. 455-461 ◽  
Author(s):  
Mohamed A El Dawy ◽  
Mokhtar M Mabrouk ◽  
Riad A El Barbary
Keyword(s):  
Aqueous Solutions ◽  
Human Plasma ◽  
Plasma Samples ◽  
Spiked Human Plasma ◽  
Human Plasma Samples ◽  
Warfarin Sodium ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Methylene Groups

Abstract A spectrofluorimetric method is described for the determination of drugs containing active methylene groups adjacent to carbonyl groups. The method was applied successfully to the determination of warfarin sodium in laboratory-prepared mixtures, in commercial tablets, and in spiked human plasma samples. Finally, the method was applied to the determination of the steady-state concentration of warfarin sodium in the blood of a hospitalized patient. The method involves the reaction of warfarin sodium with 0.2 ml (0.4 × 10−3M) N1-methylnicotinamide chloride reagent in the presence of 3 mL 1.0N NaOH and cooling in ice for 8 min, followed by adjustment of the pH to 2.0, using formic acid and heating for 4 min, whereby a highly fluorescent reaction product is produced. The optimal wavelengths of excitation and emission were determined by using a synchronous wavelength search and found to be 284 and 354 nm, respectively. The standard curves were linear over a concentration range of 50–1500 ng/mL in both aqueous solutions and spiked human plasma samples. The mean recoveries (± standard deviation) were 101.157 (±1.33) and 95.73 (±1.88%) for aqueous solutions and spiked human plasma samples, respectively. The method showed good specificity and precision. The proposed method is simple and economical because of its minimal instrumentation and chemicals requirements. Nevertheless, it is highly sensitive, specific, and reproducible. Accordingly, it is suitable for quality-control applications, drug monitoring, and bioavailability and bioequivalency studies.

Enhanced spectrofluorimetric determination of esomeprazole and pantoprazole in dosage forms and spiked human plasma using organized media

Luminescence ◽  
2014 ◽  
Vol 30 (3) ◽  
pp. 343-351 ◽  
Author(s):  
Fathalla Belal ◽  
Mohie Sharaf EL-Din ◽  
Manar M. Tolba ◽  
Heba Alaa
Keyword(s):  
Human Plasma ◽  
Dosage Forms ◽  
Spiked Human Plasma ◽  
Spectrofluorimetric Determination ◽  
Organized Media

scholarly journals The Oxidative Derivatization Method For The Spectrofluorimetric Determination Of Periciazine In Pharmaceutical Preparations

2020 ◽  
Vol 15 (1) ◽  
pp. 21-26
Author(s):  
M.Ye. Blazheyevskiy ◽  
Yu.V. Scrypynets ◽  
A.V. Yegorova ◽  
V.P. Antonovich
Keyword(s):  
Hydrochloric Acid ◽  
Acid Solution ◽  
Quantitative Determination ◽  
Pharmaceutical Preparations ◽  
Oral Solution ◽  
Calibration Plot ◽  
Highly Sensitive ◽  
Spectrofluorimetric Determination ◽  
Derivatizing Agent

The oxidative derivatization method for the indirect spectrofluorimetric determination of Periciazine has been presented. Potassium hydrogenperoxymonosulfate (Oxone ®) is proposed as a derivatizing agent for Periciazine, yielding the strongly fluorescent Periciazine sulfoxide. A highly sensitive, simple and rapid method for determination of the Periciazine by fluorescence of its oxidation product with Oxone ® solution in 0.02 M hydrochloric acid solution (λex = 364 nm; λem = 444 nm) has been developed. The calibration plot is linear in concentration range of 0.05 – 4.00 µg mL -1 . LOQ (10S) is 0.05 µg mL -1 . The possibility of quantitative determination of Periciazine in pharmaceutical preparations (Neuleptil ®, 10 mg capsules and Neuleptil ®, a 30 mL 4 % oral (solution) drops) has been shown RSD < 2.2 % (δ < RSD).

scholarly journals Facile complexation reactions for the selective spectrofluorimetric determination of albendazole in oral dosage forms and spiked human plasma

RSC Advances ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 5373-5381 ◽  
Author(s):  
Ahmed A. Hamad ◽  
Ramadan Ali ◽  
Hassan Refat H. Ali ◽  
Dalia M. Nagy ◽  
Sayed M. Derayea
Keyword(s):  
Human Plasma ◽  
Dosage Forms ◽  
Oral Dosage ◽  
Native Fluorescence ◽  
Spiked Human Plasma ◽  
Erythrosine B ◽  
Spectrofluorimetric Determination ◽  
Complexation Reactions ◽  
Oral Dosage Forms

Complexation of albendazole with erythrosine B quench the native fluorescence of the dye while complexation of the drug with lanthanum (iii) ions enhance the fluorescence of the drug.

Sign in / Sign up

Export Citation Format

Share Document