Hepatic encephalopathy induces site-specific changes in gene expression of GluN1 subunit of NMDA receptor in rat brain

2015 ◽  
Vol 30 (4) ◽  
pp. 1035-1041 ◽  
Author(s):  
Shamseddin Ahmadi ◽  
Mahsa Poureidi ◽  
Jalal Rostamzadeh
Keyword(s):  
Gene Expression ◽  
Hepatic Encephalopathy ◽  
Nmda Receptor ◽  
Rat Brain ◽  
Site Specific

scholarly journals The NMDA Receptor Subunit (GluN1 and GluN2A) Modulation Following Different Conditions of Cocaine Abstinence in Rat Brain Structures

2021 ◽  
Author(s):  
Irena Smaga ◽  
Karolina Wydra ◽  
Agata Suder ◽  
Małgorzata Frankowska ◽  
Marek Sanak ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nmda Receptor ◽  
Rat Brain ◽  
Brain Structures ◽  
Extinction Training ◽  
Receptor Subunit ◽  
Nmda Receptor Subunit ◽  
Cocaine Seeking ◽  
Instrumental Task ◽  
Seeking Behavior

AbstractDifferent neuronal alterations within glutamatergic system seem to be crucial for developing of cocaine-seeking behavior. Cocaine exposure provokes a modulation of the NMDA receptor subunit expression in rodents, which probably contributes to cocaine-induced behavioral alterations. The aim of this study was to examine the composition of the NMDA receptor subunits in the brain structures in rats with the history of cocaine self-administration after cocaine abstinence (i) in an enriched environment, (ii) in an isolated condition, (iii) with extinction training, or (iv) without instrumental task, as well as the Grin1 (encoding GluN1) and Grin2A (encoding GluN2A) gene expression were evaluated after 10-day extinction training in rat brain structures. In the present study, we observed changes only following cocaine abstinence with extinction training, when the increased GluN2A subunit levels were seen in the postsynaptic density fraction but not in the whole homogenate of the prelimbic cortex (PLC) and dorsal hippocampus (dHIP) in rats previously self-administered cocaine. At the same time, extinction training did not change the Grin1 and Grin2A gene expression in these structures. In conclusion, NMDA receptor subunit modulation observed following cocaine abstinence with extinction training may represent a potential target in cocaine-seeking behavior.

Changes in NMDA-receptor gene expression are associated with neurotoxicity induced neonatally by glutamate in the rat brain

2001 ◽  
Vol 39 (1) ◽  
pp. 1-10 ◽  
Author(s):  
C Beas-Zárate ◽  
S.V Rivera-Huizar ◽  
A Martinez-Contreras ◽  
A Feria-Velasco ◽  
J Armendariz-Borunda
Keyword(s):  
Gene Expression ◽  
Nmda Receptor ◽  
Rat Brain ◽  
Receptor Gene ◽  
Receptor Gene Expression

NMDA receptor mediated changes in IGF-II gene expression in the rat brain after injury and the possible role of nitric oxide

2000 ◽  
Vol 26 (6) ◽  
pp. 513-521 ◽  
Author(s):  
M. Giannakopoulou ◽  
M. Mansour ◽  
E. Kazanis ◽  
E. Bozas ◽  
H. Philpipidis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitric Oxide ◽  
Nmda Receptor ◽  
Rat Brain

Use of Both Fluoro-Jade B and Hematoxylin and Eosin to Detect Cell Death in the Juvenile Rat Brain Exposed to NMDA-Receptor Antagonists or GABA-Receptor Agonists in Safety Assessment

2021 ◽  
pp. 019262332110077
Author(s):  
Catherine A. Picut ◽  
Odete R. Mendes ◽  
David S. Weil ◽  
Sarah Davis ◽  
Cynthia Swanson
Keyword(s):  
Cell Death ◽  
Nmda Receptor ◽  
Rat Brain ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Nmda Receptor Antagonists ◽  
Neonatal Rats ◽  
Receptor Antagonists ◽  
Fluoro Jade B ◽  
Hematoxylin And Eosin

Administration of pediatric anesthetics with N-methyl D-aspartate (NMDA)-receptor antagonist and/or γ-aminobutyric acid (GABA) agonist activities may result in neuronal degeneration and/or neuronal cell death in neonatal rats. Evaluating pediatric drug candidates for this potential neurotoxicity is often part of overall preclinical new drug development strategy. This specialized assessment may require dosing neonatal rats at postnatal day 7 at the peak of the brain growth spurt and evaluating brain tissue 24 to 48 hours following dosing. The need to identify methods to aid in the accurate and reproducible detection of lesions associated with this type of neurotoxic profile is paramount for meeting the changing needs of neuropathology assessment and addressing emerging challenges in the neuroscience field. We document the use of Fluoro-Jade B (FJB) staining, to be used in conjunction with standard hematoxylin and eosin staining, to detect acute neurodegeneration and neuronal cell death that can be caused by some NMDA-receptor antagonists and/or GABA agonists in the neonatal rat brain. The FJB staining is simple, specific, and sensitive and can be performed on brain specimens from the same cohort of animals utilized for standard neurotoxicity assessment, thus satisfying animal welfare recommendations with no effect on achievement of scientific and regulatory goals.

scholarly journals Tissue- and cell-specific casein gene expression. II. Relationship to site-specific DNA methylation.

1983 ◽  
Vol 258 (17) ◽  
pp. 10805-10811 ◽  
Author(s):  
M L Johnson ◽  
J Levy ◽  
S C Supowit ◽  
L Y Yu-Lee ◽  
J M Rosen
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Site Specific ◽  
Casein Gene
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